{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T22:27:04Z","timestamp":1782599224932,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012052","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T00:00:00Z","timestamp":1723680000000}}],"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB-1717498"],"award-info":[{"award-number":["DEB-1717498"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB-1911853"],"award-info":[{"award-number":["DEB-1911853"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"\n Avian malaria is expanding upslope with warmer temperatures and driving multiple species of Hawaiian birds towards extinction. Methods to reduce malaria transmission are urgently needed to prevent further declines. Releasing\n Wolbachia<\/jats:italic>\n -infected incompatible male mosquitoes could suppress mosquito populations and releasing\n Wolbachia<\/jats:italic>\n -infected female mosquitoes (or both sexes) could reduce pathogen transmission if the\n Wolbachia<\/jats:italic>\n strain reduced vector competence. We cleared\n Culex quinquefasciatus<\/jats:italic>\n of their natural\n Wolbachia pipientis w<\/jats:italic>\n Pip infection and transinfected them with\n Wolbachia w<\/jats:italic>\n AlbB isolated from\n Aedes albopictus<\/jats:italic>\n . We show that\n w<\/jats:italic>\n AlbB infection was transmitted transovarially, and demonstrate cytoplasmic incompatibility with wild-type mosquitoes infected with\n w<\/jats:italic>\n Pip from Oahu and Maui, Hawaii. We measured vector competence for avian malaria,\n Plasmodium relictum<\/jats:italic>\n , lineage GRW4, of seven mosquito lines (two with\n w<\/jats:italic>\n AlbB; three with natural\n w<\/jats:italic>\n Pip infection, and two cleared of\n Wolbachia<\/jats:italic>\n infection) by allowing them to feed on canaries infected with recently collected field isolates of Hawaiian\n P<\/jats:italic>\n .\n relictum<\/jats:italic>\n . We tested 73 groups (N\n total<\/jats:sub>\n = 1176) of mosquitoes for\n P<\/jats:italic>\n .\n relictum<\/jats:italic>\n infection in abdomens and thoraxes 6\u201314 days after feeding on a range of parasitemias from 0.028% to 2.49%, as well as a smaller subset of salivary glands. We found no measurable effect of\n Wolbachia<\/jats:italic>\n on any endpoint, but strong effects of parasitemia, days post feeding, and mosquito strain on both abdomen and thorax infection prevalence. These results suggest that releasing male\n w<\/jats:italic>\n AlbB-infected\n C<\/jats:italic>\n .\n quinquefasciatus<\/jats:italic>\n mosquitoes could suppress\n w<\/jats:italic>\n Pip-infected mosquito populations, but would have little positive or negative impact on mosquito vector competence for\n P<\/jats:italic>\n .\n relictum<\/jats:italic>\n if\n w<\/jats:italic>\n AlbB became established in local mosquito populations. More broadly, the lack of\n Wolbachia<\/jats:italic>\n effects on vector competence we observed highlights the variable impacts of both native and transinfected\n Wolbachia<\/jats:italic>\n infections in mosquitoes.\n <\/jats:p>","DOI":"10.1371\/journal.ppat.1012052","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T13:45:20Z","timestamp":1722865520000},"page":"e1012052","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Transinfection of Wolbachia wAlbB into Culex quinquefasciatus mosquitoes does not alter vector competence for Hawaiian avian malaria (Plasmodium relictum GRW4)"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3612-5775","authenticated-orcid":true,"given":"A. Marm","family":"Kilpatrick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christa M.","family":"Seidl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaiah J.","family":"Ipsaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris E.","family":"Garrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Fabbri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul I.","family":"Howell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin G.","family":"McGowan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley J.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara N.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"ppat.1012052.ref001","doi-asserted-by":"crossref","first-page":"e1254","DOI":"10.1002\/wsb.1254","article-title":"Minimizing extinction risk in the face of uncertainty: Developing conservation strategies for 2 rapidly declining forest bird species on Kaua\u2018i Island","volume":"46","author":"EH Paxton","year":"2022","journal-title":"Wildl Soc Bull"},{"key":"ppat.1012052.ref002","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1098\/rspb.2006.3671","article-title":"Global phylogeographic limits of Hawaii\u2019s avian malaria","volume":"273","author":"JS Beadell","year":"2006","journal-title":"Proc R Soc B Biol 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