{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:03:18Z","timestamp":1791460998658,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012127","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000}}],"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI164619"],"award-info":[{"award-number":["R21AI164619"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UL1TR002529"],"award-info":[{"award-number":["UL1TR002529"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI149766"],"award-info":[{"award-number":["R01AI149766"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK124067"],"award-info":[{"award-number":["R01DK124067"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>The single mitochondrion of the obligate intracellular parasite <jats:italic>Toxoplasma gondii<\/jats:italic> is highly dynamic. Toxoplasma\u2019s mitochondrion changes morphology as the parasite moves from the intracellular to the extracellular environment and during division. Toxoplasma\u2019s mitochondrial dynamic is dependent on an outer mitochondrion membrane-associated protein LMF1 and its interaction with IMC10, a protein localized at the inner membrane complex (IMC). In the absence of either LMF1 or IMC10, parasites have defective mitochondrial morphology and inheritance defects. As little is known about mitochondrial inheritance in <jats:italic>Toxoplasma<\/jats:italic>, we have used the LMF1\/IMC10 tethering complex as an entry point to dissect the machinery behind this process. Using a yeast two-hybrid screen, we previously identified Myosin A (MyoA) as a putative interactor of LMF1. Although MyoA is known to be located at the parasite\u2019s pellicle, we now show through ultrastructure expansion microscopy (U-ExM) that this protein accumulates around the mitochondrion in the late stages of parasite division. Parasites lacking MyoA show defective mitochondrial morphology and a delay in mitochondrion delivery to the daughter parasite buds during division, indicating that this protein is involved in organellar inheritance. Disruption of the parasite\u2019s actin network also affects mitochondrion morphology. We also show that parasite-extracted mitochondrion vesicles interact with actin filaments. Interestingly, mitochondrion vesicles extracted out of parasites lacking LMF1 pulled down less actin, showing that LMF1 might be important for mitochondrion and actin interaction. Accordingly, we are showing for the first time that actin and Myosin A are important for <jats:italic>Toxoplasma<\/jats:italic> mitochondrial morphology and inheritance.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012127","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T17:38:12Z","timestamp":1728322692000},"page":"e1012127","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii"],"prefix":"10.1371","volume":"20","author":[{"given":"Rodolpho Ornitz","family":"Oliveira Souza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4445-0375","authenticated-orcid":true,"given":"Gustavo","family":"Arrizabalaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"issue":"2","key":"ppat.1012127.ref001","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1091\/mbc.01-06-0309","article-title":"Daughter cell assembly in the protozoan parasite Toxoplasma gondii","volume":"13","author":"K Hu","year":"2002","journal-title":"Mol Biol Cell"},{"issue":"Pt 9","key":"ppat.1012127.ref002","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1242\/jcs.021089","article-title":"Organellar dynamics during the cell cycle of Toxoplasma 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Jacobs","year":"2020","journal-title":"mBio"},{"issue":"22","key":"ppat.1012127.ref006","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.260083","article-title":"IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii","volume":"135","author":"RO Oliveira Souza","year":"2022","journal-title":"J Cell Sci"},{"issue":"7851","key":"ppat.1012127.ref007","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/s41586-021-03309-5","article-title":"Actin cables and comet tails organize mitochondrial networks in mitosis","volume":"591","author":"AS Moore","year":"2021","journal-title":"Nature"},{"issue":"1","key":"ppat.1012127.ref008","doi-asserted-by":"crossref","first-page":"5924","DOI":"10.1038\/s41598-019-42441-1","article-title":"A plasma membrane localized protein phosphatase in Toxoplasma gondii, PPM5C, regulates attachment to host cells.","volume":"9","author":"C Yang","year":"2019","journal-title":"Sci 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