{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:45:57Z","timestamp":1787510757453,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012151","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000}}],"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18H02650 and JP21H02723, JPJSCCB20190010"],"award-info":[{"award-number":["JP18H02650 and JP21H02723, JPJSCCB20190010"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K06514"],"award-info":[{"award-number":["23K06514"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["Bilateral Joint Research Grant"],"award-info":[{"award-number":["Bilateral Joint Research Grant"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0108138"],"award-info":[{"award-number":["JP20fk0108138"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Japan Agency for Medical Research and Development (AMED):","award":["JP223fa627001"],"award-info":[{"award-number":["JP223fa627001"]}]},{"name":"Tokyo Medical Society"},{"DOI":"10.13039\/501100004721","name":"University of Tokyo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004721","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Tetraspanins (TSPANs) are a family of highly conserved proteins present in a wide variety of eukaryotes. Although protein-protein interactions of TSPANs have been well established in eukaryotes including parasitic protists, the role they play in parasitism and pathogenesis remains largely unknown. In this study, we characterized three representative members of TSPANs, TSPAN4, TSPAN12, and TSPAN13 from the human intestinal protozoan\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    . Co-immunoprecipitation assays demonstrated that TSPAN4, TSPAN12 and TSPAN13 are reciprocally pulled down together with several other TSPAN-interacting proteins including TSPAN binding protein of 55kDa (TBP55) and interaptin. Blue native-PAGE analysis showed that these TSPANs form several complexes of 120\u2013250 kDa. Repression of\n                    <jats:italic>tspan12<\/jats:italic>\n                    and\n                    <jats:italic>tspan13<\/jats:italic>\n                    gene expression led to decreased secretion of cysteine proteases, while repression of\n                    <jats:italic>tspan4<\/jats:italic>\n                    led to a four-fold increase in the activity of cysteine proteases in crude extracellular vesicles (EVs) fraction. Meanwhile, strains overexpressing HA-tagged TSPAN12 and TSPAN13 demonstrated reduced adhesion to collagen. Altogether, this study reveals that the TSPANs, especially TSPAN12 and TSPAN13, are engaged with complex protein-protein interactions and are involved in the pathogenicity-related biological functions such as protease secretion and adhesion, offering insights into the potential regulatory mechanisms of tetraspanins in protozoan parasites.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1012151","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T13:45:32Z","timestamp":1727963132000},"page":"e1012151","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5259-0435","authenticated-orcid":true,"given":"Han","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4322-0145","authenticated-orcid":true,"given":"Herbert J.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1354-5133","authenticated-orcid":true,"given":"Tomoyoshi","family":"Nozaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,10,3]]},"reference":[{"issue":"1","key":"ppat.1012151.ref001","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1158\/1541-7786.MCR-12-0468","article-title":"Genetic ablation of the tetraspanin CD151 reduces spontaneous metastatic spread of prostate cancer in 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