{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:59:01Z","timestamp":1791410341586,"version":"4.3.3"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012175","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000}}],"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["ZIAAI001086"],"award-info":[{"award-number":["ZIAAI001086"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P30 AG072976"],"award-info":[{"award-number":["P30 AG072976"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022077","name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Animal Health"},{"DOI":"10.13039\/100007917","name":"USDA-ARS","doi-asserted-by":"crossref","award":["2090-32000-042-000-D"],"award-info":[{"award-number":["2090-32000-042-000-D"]}],"id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01 NS 083687 and P01 A1106705"],"award-info":[{"award-number":["R01 NS 083687 and P01 A1106705"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Prions or prion-like aggregates such as those composed of PrP, \u03b1-synuclein, and tau are key features of proteinopathies such as prion, Parkinson\u2019s and Alzheimer\u2019s diseases, respectively. Their presence on solid surfaces may be biohazardous under some circumstances. PrP prions bound to solids are detectable by ultrasensitive real-time quaking-induced conversion (RT-QuIC) assays if the solids can be immersed in assay wells or the prions transferred to pads. Here we show that prion-like seeds can remain detectable on steel wires for at least a year, or even after enzymatic cleaning and sterilization. We also show that contamination of larger objects with pathological seeds of \u03b1-synuclein, tau, and PrP can be detected by simply assaying a sampling medium that has been transiently applied to the surface. Human \u03b1-synuclein seeds in dementia with Lewy bodies brain tissue were detected by \u03b1-synuclein RT-QuIC after drying of tissue dilutions with concentrations as low as 10<jats:sup>\u22126<\/jats:sup> onto stainless steel. Tau RT-QuIC detected tau seeding activity on steel exposed to Alzheimer\u2019s disease brain tissue diluted as much as a billion fold. Prion RT-QuIC assays detected seeding activity on plates exposed to brain dilutions as extreme as 10<jats:sup>\u22125<\/jats:sup>\u201310<jats:sup>\u22128<\/jats:sup> from prion-affected humans, sheep, cattle and cervids. Sampling medium collected from surgical instruments used in necropsies of sporadic Creutzfeldt-Jakob disease-infected transgenic mice was positive down to 10<jats:sup>\u22126<\/jats:sup> dilution. Sensitivity for prion detection was not sacrificed by omitting the recombinant PrP substrate from the sampling medium during its application to a surface and subsequent storage as long as the substrate was added prior to performing the assay reaction. Our findings demonstrate practical prototypic surface RT-QuIC protocols for the highly sensitive detection of pathologic seeds of \u03b1-synuclein, tau, and PrP on solid objects.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012175","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T17:28:11Z","timestamp":1713547691000},"page":"e1012175","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Sensitive detection of pathological seeds of \u03b1-synuclein, tau and prion protein on solid surfaces"],"prefix":"10.1371","volume":"20","author":[{"given":"Christina D.","family":"Orr\u00fa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley R.","family":"Groveman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew G.","family":"Hughson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s","family":"Barrio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kachi","family":"Isiofia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent","family":"Race","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia C.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierluigi","family":"Gambetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Masujin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohtaro","family":"Miyazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardino","family":"Ghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluigi","family":"Zanusso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3928-6101","authenticated-orcid":true,"given":"Byron","family":"Caughey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"issue":"7980","key":"ppat.1012175.ref001","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/s41586-023-06437-2","article-title":"Molecular pathology of neurodegenerative diseases by cryo-EM of amyloids","volume":"621","author":"SHW Scheres","year":"2023","journal-title":"Nature"},{"issue":"7628","key":"ppat.1012175.ref002","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/nature20415","article-title":"Mammalian prions and their wider relevance in neurodegenerative diseases","volume":"539","author":"J. 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