{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:19:56Z","timestamp":1790515196602,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012189","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T00:00:00Z","timestamp":1715904000000}}],"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI173244, R21AI166642"],"award-info":[{"award-number":["R01AI173244, R21AI166642"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32AI007401"],"award-info":[{"award-number":["T32AI007401"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM127261"],"award-info":[{"award-number":["T32GM127261"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wellcome Fund"},{"DOI":"10.13039\/100008478","name":"Wake Forest School of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Successful microbial colonization of the gastrointestinal (GI) tract hinges on an organism\u2019s ability to overcome the intense competition for nutrients in the gut between the host and the resident gut microbiome. Enteric pathogens can exploit ethanolamine (EA) in the gut to bypass nutrient competition. However, <jats:italic>Klebsiella pneumoniae<\/jats:italic> (<jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic>) is an asymptomatic gut colonizer and, unlike well-studied enteric pathogens, harbors two genetically distinct ethanolamine utilization (<jats:italic>eut<\/jats:italic>) loci. Our investigation uncovered unique roles for each <jats:italic>eut<\/jats:italic> locus depending on EA utilization as a carbon or nitrogen source. Murine gut colonization studies demonstrated the necessity of both <jats:italic>eut<\/jats:italic> loci in the presence of intact gut microbiota for robust GI colonization by <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic>. Additionally, while some <jats:italic>Escherichia coli<\/jats:italic> gut isolates could metabolize EA, other commensals were incapable, suggesting that EA metabolism likely provides <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> a selective advantage in gut colonization. Molecular and bioinformatic analyses unveiled the conservation of two <jats:italic>eut<\/jats:italic> loci among <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> and a subset of the related taxa in the <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> species complex, with the NtrC-RpoN regulatory cascade playing a pivotal role in regulation. These findings identify EA metabolism as a critical driver of <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> niche establishment in the gut and propose microbial metabolism as a potential therapeutic avenue to combat <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> infections.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012189","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T17:37:34Z","timestamp":1715103454000},"page":"e1012189","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Ethanolamine metabolism through two genetically distinct loci enables Klebsiella pneumoniae to bypass nutritional competition in the gut"],"prefix":"10.1371","volume":"20","author":[{"given":"Andrew J.","family":"Barnes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma F.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Vezina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew W.","family":"Hudson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna E.","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah A.","family":"Nutter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Bray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravinder","family":"Nagpal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly L.","family":"Wyres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3737-0422","authenticated-orcid":true,"given":"M. Ammar","family":"Zafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,5,7]]},"reference":[{"key":"ppat.1012189.ref001","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1042\/BCJ20160510","article-title":"Introduction to the human gut microbiota","volume":"474","author":"E Thursby","year":"2017","journal-title":"Biochem J"},{"key":"ppat.1012189.ref002","doi-asserted-by":"crossref","first-page":"R538","DOI":"10.1016\/j.cub.2019.04.017","article-title":"Understanding Competition and Cooperation within the Mammalian Gut Microbiome","volume":"29","author":"KZ Coyte","year":"2019","journal-title":"Curr Biol"},{"key":"ppat.1012189.ref003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41385-018-0053-0","article-title":"Interbacterial mechanisms of colonization resistance and the strategies pathogens use to overcome them","volume":"12","author":"MT Sorbara","year":"2019","journal-title":"Mucosal Immunol"},{"key":"ppat.1012189.ref004","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1111\/1462-2920.13659","article-title":"Microbial nutrient niches in the gut","volume":"19","author":"FC Pereira","year":"2017","journal-title":"Environ Microbiol"},{"key":"ppat.1012189.ref005","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.716299","article-title":"Mechanism of the Gut Microbiota Colonization Resistance and Enteric Pathogen Infection.","volume":"11","author":"I Khan","year":"2021","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"ppat.1012189.ref006","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/nature18849","article-title":"Interactions between the microbiota and pathogenic bacteria in the gut","volume":"535","author":"AJ Baumler","year":"2016","journal-title":"Nature"},{"key":"ppat.1012189.ref007","author":"C Centers for Disease","journal-title":"Prevention. 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