{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:15:48Z","timestamp":1789676148820,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012303","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000}}],"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:00:00Z","timestamp":1718582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI153410"],"award-info":[{"award-number":["R01AI153410"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p><jats:italic>Chlamydia trachomatis<\/jats:italic> is a clinically important bacterium that infects epithelial cells of the genitourinary and respiratory tracts and the eye. These differentiated cells are in a quiescent growth state and have a surface organelle called a primary cilium, but the standard <jats:italic>Chlamydia<\/jats:italic> cell culture infection model uses cycling cells that lack primary cilia. To investigate if these differences are relevant, we performed infections with host cells that have a primary cilium. We found that <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> caused progressive loss of the primary cilium that was prevented by disrupting Aurora A (AurA), HDAC6 or calmodulin, which are components of the cellular cilia disassembly pathway. Stabilization of the primary cilium by targeting this pathway caused a large reduction in infectious progeny although there were no changes in chlamydial inclusion growth, chlamydial replication or the ultrastructural appearance of dividing and infectious forms (RBs and EBs, respectively). Thus, the presence of a primary cilium interfered with the production of infectious EBs at a late step in the developmental cycle. <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> infection also induced quiescent cells to re-enter the cell cycle, as detected by EdU incorporation in S-phase, and <jats:italic>Chlamydia<\/jats:italic>-induced cilia disassembly was necessary for cell cycle re-entry. This study therefore describes a novel host-pathogen interaction in which the primary cilium limits a productive <jats:italic>Chlamydia<\/jats:italic> infection, and the bacterium counteracts this host cell defense by activating the cellular cilia disassembly pathway.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012303","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T18:12:53Z","timestamp":1718647973000},"page":"e1012303","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Chlamydia trachomatis induces disassembly of the primary cilium to promote the intracellular infection"],"prefix":"10.1371","volume":"20","author":[{"given":"Roseleen","family":"Ekka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Gutierrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten A.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5361-583X","authenticated-orcid":true,"given":"Ming","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1354-507X","authenticated-orcid":true,"given":"Christine","family":"S\u00fctterlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"key":"ppat.1012303.ref001","first-page":"2301","volume-title":"Mandell, Douglas, and Bennett\u2019s: Principles and Practice of Infectious Diseases. 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