{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:59:33Z","timestamp":1792587573641,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012388","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T00:00:00Z","timestamp":1723680000000}}],"reference-count":85,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004412","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["CDA00047\/2017 C"],"award-info":[{"award-number":["CDA00047\/2017 C"]}],"id":[{"id":"10.13039\/100004412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007067","name":"Kempe Foundations","doi-asserted-by":"crossref","award":["Postdoctoral fellowship"],"award-info":[{"award-number":["Postdoctoral fellowship"]}],"id":[{"id":"10.13039\/501100007067","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["101033469"],"award-info":[{"award-number":["101033469"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004063","name":"Knut and Alice Wallenberg Foundation","doi-asserted-by":"crossref","award":["2018\u201305851"],"award-info":[{"award-number":["2018\u201305851"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Swedish research council","award":["2021\u201301145"],"award-info":[{"award-number":["2021\u201301145"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Enteroviruses are a vast genus of positive-sense RNA viruses that cause diseases ranging from common cold to poliomyelitis and viral myocarditis. They encode a membrane-bound AAA+ ATPase, 2C, that has been suggested to serve several roles in virus replication, e.g. as an RNA helicase and capsid assembly factor. Here, we report the reconstitution of full-length, poliovirus 2C\u2019s association with membranes. We show that the N-terminal membrane-binding domain of 2C contains a conserved glycine, which is suggested by structure predictions to divide the domain into two amphipathic helix regions, which we name AH1 and AH2. AH2 is the main mediator of 2C oligomerization, and is necessary and sufficient for its membrane binding. AH1 is the main mediator of a novel function of 2C: clustering of membranes. Cryo-electron tomography reveal that several 2C copies mediate this function by localizing to vesicle-vesicle interfaces. 2C-mediated clustering is partially outcompeted by RNA, suggesting a way by which 2C can switch from an early role in coalescing replication organelles and lipid droplets, to a later role where 2C assists RNA replication and particle assembly. 2C is sufficient to recruit RNA to membranes, with a preference for double-stranded RNA (the replicating form of the viral genome). Finally, the <jats:italic>in vitro<\/jats:italic> reconstitution revealed that full-length, membrane-bound 2C has ATPase activity and ATP-independent, single-strand ribonuclease activity, but no detectable helicase activity. Together, this study suggests novel roles for 2C in membrane clustering, RNA membrane recruitment and cleavage, and calls into question a role of 2C as an RNA helicase. The reconstitution of functional, 2C-decorated vesicles provides a platform for further biochemical studies into this protein and its roles in enterovirus replication.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012388","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T17:46:06Z","timestamp":1722879966000},"page":"e1012388","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["In vitro reconstitution reveals membrane clustering and RNA recruitment by the enteroviral AAA+ ATPase 2C"],"prefix":"10.1371","volume":"20","author":[{"given":"Kasturika","family":"Shankar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie N.","family":"Sorin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himanshu","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oskar","family":"Skoglund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma","family":"Dahmane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josy","family":"ter Beek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solomon","family":"Tesfalidet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Nenz\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2342-6488","authenticated-orcid":true,"given":"Lars-Anders","family":"Carlson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"ppat.1012388.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/0022-1317-78-1-1","article-title":"Classification of enteroviruses based on molecular and biological properties","volume":"78","author":"T Hyypia","year":"1997","journal-title":"J Gen Virol"},{"issue":"3","key":"ppat.1012388.ref002","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1128\/MMBR.00012-14","article-title":"Picornavirus morphogenesis","volume":"78","author":"P Jiang","year":"2014","journal-title":"Microbiol Mol Biol Rev"},{"issue":"7","key":"ppat.1012388.ref003","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1007\/s00705-015-2425-z","article-title":"Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2015)","volume":"160","author":"MJ Adams","year":"2015","journal-title":"Arch Virol"},{"issue":"10271","key":"ppat.1012388.ref004","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/S0140-6736(20)32723-9","article-title":"Acute flaccid myelitis: cause, diagnosis, and management","volume":"397","author":"OC Murphy","year":"2021","journal-title":"Lancet"},{"issue":"5","key":"ppat.1012388.ref005","doi-asserted-by":"crossref","first-page":"e64","DOI":"10.1016\/S1473-3099(15)00543-5","article-title":"Global emergence of enterovirus D68: a systematic review","volume":"16","author":"CC Holm-Hansen","year":"2016","journal-title":"Lancet Infect Dis"},{"issue":"19","key":"ppat.1012388.ref006","doi-asserted-by":"crossref","first-page":"10423","DOI":"10.1128\/JVI.77.19.10423-10431.2003","article-title":"Recombination in circulating enteroviruses","volume":"77","author":"AN Lukashev","year":"2003","journal-title":"J Virol"},{"issue":"1","key":"ppat.1012388.ref007","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.virol.2004.04.026","article-title":"Molecular comparison of echovirus 11 strains circulating in Europe during an epidemic of multisystem hemorrhagic disease of infants indicates that evolution generally occurs by recombination","volume":"325","author":"S Chevaliez","year":"2004","journal-title":"Virology"},{"issue":"3","key":"ppat.1012388.ref008","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/rmv.457","article-title":"Role of recombination in evolution of enteroviruses","volume":"15","author":"AN Lukashev","year":"2005","journal-title":"Rev Med Virol"},{"issue":"47","key":"ppat.1012388.ref009","doi-asserted-by":"crossref","first-page":"6802","DOI":"10.1016\/j.vaccine.2022.09.088","article-title":"The fight against poliomyelitis through the history: past, present and hopes for the future. 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