{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:00:48Z","timestamp":1790802048230,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012411","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000}}],"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:00Z","timestamp":1721260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI123360"],"award-info":[{"award-number":["AI123360"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM153408"],"award-info":[{"award-number":["GM153408"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM007185"],"award-info":[{"award-number":["GM007185"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI007323"],"award-info":[{"award-number":["AI007323"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Molecular Biology Institute, University of California, Los Angeles","award":["Whitcome Pre-doctoral Fellowship"],"award-info":[{"award-number":["Whitcome Pre-doctoral Fellowship"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> divides by endodyogeny, in which two daughter buds are formed within the cytoplasm of the maternal cell using the inner membrane complex (IMC) as a scaffold. During endodyogeny, components of the IMC are synthesized and added sequentially to the nascent daughter buds in a tightly regulated manner. We previously showed that the early recruiting proteins IMC32 and IMC43 form an essential daughter bud assembly complex which lays the foundation of the daughter cell scaffold in <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>. In this study, we identify the essential, early recruiting IMC protein BCC0 as a third member of this complex by using IMC32 as bait in both proximity labeling and yeast two-hybrid screens. We demonstrate that BCC0\u2019s localization to daughter buds depends on the presence of both IMC32 and IMC43. Deletion analyses and functional complementation studies reveal that residues 701\u2013877 of BCC0 are essential for both its localization and function and that residues 1\u2013899 are sufficient for function despite minor mislocalization. Pairwise yeast two-hybrid assays additionally demonstrate that BCC0\u2019s essential domain binds to the coiled-coil region of IMC32 and that BCC0 and IMC43 do not directly interact. This data supports a model for complex assembly in which an IMC32-BCC0 subcomplex initially recruits to nascent buds via palmitoylation of IMC32 and is locked into the scaffold once bud elongation begins by IMC32 binding to IMC43. Together, this study dissects the organization and function of a complex of three early recruiting daughter proteins which are essential for the proper assembly of the IMC during endodyogeny.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012411","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T17:58:12Z","timestamp":1721325492000},"page":"e1012411","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["BCC0 collaborates with IMC32 and IMC43 to form the Toxoplasma gondii essential daughter bud assembly complex"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0041-5623","authenticated-orcid":true,"given":"Rebecca R.","family":"Pasquarelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihui","family":"Sha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Wohlschlegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8481-0638","authenticated-orcid":true,"given":"Peter J.","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"key":"ppat.1012411.ref001","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1079\/AHR2005100","article-title":"Biology and epidemiology of Toxoplasma gondii in man and animals","volume":"6","author":"DE Hill","year":"2005","journal-title":"Anim Health Res Rev"},{"key":"ppat.1012411.ref002","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.pt.2004.09.006","article-title":"Clinical features and pathogenesis of severe malaria","volume":"20","author":"CL Mackintosh","year":"2004","journal-title":"Trends Parasitol"},{"key":"ppat.1012411.ref003","doi-asserted-by":"crossref","first-page":"e0004729","DOI":"10.1371\/journal.pntd.0004729","article-title":"The Burden of Cryptosporidium Diarrheal Disease among Children < 24 Months of Age in Moderate\/High Mortality Regions of Sub-Saharan Africa and South Asia, Utilizing Data from the Global Enteric Multicenter Study (GEMS).","volume":"10","author":"SO Sow","year":"2016","journal-title":"PLoS Negl Trop Dis."},{"key":"ppat.1012411.ref004","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/j.1365-3024.2010.01209.x","article-title":"Chasing the golden egg: vaccination against poultry coccidiosis","volume":"32","author":"PA Sharman","year":"2010","journal-title":"Parasite Immunol"},{"key":"ppat.1012411.ref005","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s11250-006-4181-2","article-title":"Estimated direct economic costs associated with tick-borne diseases on cattle in Tanzania.","volume":"38","author":"FM Kivaria","year":"2006","journal-title":"Trop Anim Health Prod"},{"key":"ppat.1012411.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3347\/kjp.2003.41.1.1","article-title":"Review of Neospora caninum and neosporosis in animals","volume":"41","author":"JP Dubey","year":"2003","journal-title":"Korean J Parasitol"},{"key":"ppat.1012411.ref007","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis","volume":"39","author":"G Pappas","year":"2009","journal-title":"Int J Parasitol"},{"key":"ppat.1012411.ref008","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"AM Tenter","year":"2000","journal-title":"Int J Parasitol"},{"key":"ppat.1012411.ref009","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/clinids\/15.2.211","article-title":"Toxoplasmic Encephalitis in AIDS","volume":"15","author":"BJ Luft","year":"1992","journal-title":"Clin Infect Dis"},{"key":"ppat.1012411.ref010","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1891\/0730-0832.20.4.23","article-title":"Congenital Toxoplasmosis","volume":"20","author":"S. Martin","year":"2001","journal-title":"Neonatal Netw"},{"key":"ppat.1012411.ref011","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1146\/annurev-micro-091014-104100","article-title":"Lytic Cycle of Toxoplasma gondii: 15 Years Later","volume":"69","author":"IJ Blader","year":"2015","journal-title":"Annu Rev Microbiol"},{"key":"ppat.1012411.ref012","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/0020-7519(77)90014-5","article-title":"Comparative electron microscope study of pellicular structures in coccidia (Sarcocystis, Besnoitia and Eimeria).","volume":"7","author":"J D\u2019Haese","year":"1977","journal-title":"Int J Parasitol"},{"key":"ppat.1012411.ref013","doi-asserted-by":"crossref","first-page":"e1000270","DOI":"10.1371\/journal.ppat.1000270","article-title":"Rab11A-Controlled Assembly of the Inner Membrane Complex Is Required for Completion of Apicomplexan Cytokinesis.","volume":"5","author":"C Agop-Nersesian","year":"2009","journal-title":"PLoS Pathog"},{"key":"ppat.1012411.ref014","first-page":"e02357","article-title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","volume":"6","author":"AL Chen","year":"2015","journal-title":"mBio"},{"key":"ppat.1012411.ref015","doi-asserted-by":"crossref","first-page":"e12678","DOI":"10.1111\/cmi.12678","article-title":"Novel insights into the composition and function of the Toxoplasma IMC sutures","volume":"19","author":"AL Chen","year":"2016","journal-title":"Cell Microbiol"},{"key":"ppat.1012411.ref016","doi-asserted-by":"crossref","first-page":"e1001094","DOI":"10.1371\/journal.ppat.1001094","article-title":"A Novel Family of Toxoplasma IMC Proteins Displays a Hierarchical Organization and Functions in Coordinating Parasite Division.","volume":"6","author":"JR Beck","year":"2010","journal-title":"PLoS Pathog."},{"key":"ppat.1012411.ref017","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.molbiopara.2012.05.002","article-title":"Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation","volume":"184","author":"C Fung","year":"2012","journal-title":"Mol Biochem Parasitol"},{"key":"ppat.1012411.ref018","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0166-6851(01)00289-4","article-title":"Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite Toxoplasma gondii","volume":"115","author":"T Mann","year":"2001","journal-title":"Mol Biochem Parasitol"},{"key":"ppat.1012411.ref019","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.1462-5822.2010.01514.x","article-title":"A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of Toxoplasma gondii: IMC proteins in Toxoplasma cell division","volume":"13","author":"BR Anderson-White","year":"2011","journal-title":"Cell Microbiol"},{"key":"ppat.1012411.ref020","doi-asserted-by":"crossref","first-page":"e3000475","DOI":"10.1371\/journal.pbio.3000475","article-title":"A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex","volume":"17","author":"CP Choi","year":"2019","journal-title":"PLOS Biol"},{"key":"ppat.1012411.ref021","doi-asserted-by":"crossref","first-page":"e0286421","DOI":"10.1128\/mbio.02864-21","article-title":"Multivalent Interactions Drive the Toxoplasma AC9:AC10:ERK7 Complex To Concentrate ERK7 in the Apical Cap.","volume":"13","author":"PS Back","year":"2022","journal-title":"mBio"},{"key":"ppat.1012411.ref022","doi-asserted-by":"crossref","first-page":"12164","DOI":"10.1073\/pnas.1921245117","article-title":"Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis","volume":"117","author":"PS Back","year":"2020","journal-title":"Proc Natl Acad Sci"},{"key":"ppat.1012411.ref023","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1083\/jcb.200311137","article-title":"Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondii","volume":"165","author":"E Gaskins","year":"2004","journal-title":"J Cell Biol"},{"key":"ppat.1012411.ref024","first-page":"2023.08.10.552545","article-title":"The Toxoplasma subpellicular network is highly interconnected and defines parasite shape for efficient motility and replication.","author":"PS Back","year":"2023","journal-title":"BioRxiv Prepr Serv Biol"},{"key":"ppat.1012411.ref025","doi-asserted-by":"crossref","first-page":"882166","DOI":"10.3389\/fcimb.2022.882166","article-title":"Toxoplasma gondii\u2019s Basal Complex: The Other Apicomplexan Business End Is Multifunctional.","volume":"12","author":"M-J Gubbels","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"ppat.1012411.ref026","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/j.1550-7408.1987.tb03162.x","article-title":"Cytoskeleton of Toxoplasma gondii","volume":"34","author":"BA Nichols","year":"1987","journal-title":"J Protozool"},{"key":"ppat.1012411.ref027","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3389\/fcimb.2020.00269","article-title":"Fussing About Fission: Defining Variety Among Mainstream and Exotic Apicomplexan Cell Division Modes.","volume":"10","author":"M-J Gubbels","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"ppat.1012411.ref028","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nrmicro3184","article-title":"Cell division in apicomplexan parasites","volume":"12","author":"ME Francia","year":"2014","journal-title":"Nat Rev Microbiol"},{"key":"ppat.1012411.ref029","doi-asserted-by":"crossref","first-page":"15710","DOI":"10.1038\/ncomms15710","article-title":"Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii.","volume":"8","author":"K Fr\u00e9nal","year":"2017","journal-title":"Nat Commun."},{"key":"ppat.1012411.ref030","doi-asserted-by":"crossref","first-page":"ar37","DOI":"10.1091\/mbc.E23-11-0418","article-title":"The initiation and early development of the tubulin-containing cytoskeleton in the human parasite Toxoplasma gondii","volume":"35","author":"LFA Padilla","year":"2024","journal-title":"Mol Biol Cell"},{"key":"ppat.1012411.ref031","first-page":"3344","article-title":"The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant","volume":"126","author":"C-T Chen","year":"2013","journal-title":"J Cell Sci"},{"key":"ppat.1012411.ref032","doi-asserted-by":"crossref","first-page":"e1002093","DOI":"10.1371\/journal.pbio.1002093","article-title":"A Novel Bipartite Centrosome Coordinates the Apicomplexan Cell Cycle. Pellman D, editor","volume":"13","author":"ES Suvorova","year":"2015","journal-title":"PLOS Biol"},{"key":"ppat.1012411.ref033","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-394309-5.00001-8","article-title":"Cytoskeleton assembly in Toxoplasma gondii cell division","volume":"298","author":"B Anderson-White","year":"2012","journal-title":"Int Rev Cell Mol Biol"},{"key":"ppat.1012411.ref034","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1242\/jcs.115.5.1017","article-title":"Disruption of microtubules uncouples budding and nuclear division in Toxoplasma gondii","volume":"115","author":"NS Morrissette","year":"2002","journal-title":"J Cell Sci"},{"key":"ppat.1012411.ref035","doi-asserted-by":"crossref","first-page":"e10","DOI":"10.1371\/journal.ppat.0040010","article-title":"Organizational Changes of the Daughter Basal Complex during the Parasite Replication of Toxoplasma gondii.","volume":"4","author":"K. Hu","year":"2008","journal-title":"PLoS Pathog."},{"key":"ppat.1012411.ref036","doi-asserted-by":"crossref","first-page":"e12354","DOI":"10.1371\/journal.pone.0012354","article-title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.","volume":"5","author":"MS Behnke","year":"2010","journal-title":"PloS One"},{"key":"ppat.1012411.ref037","doi-asserted-by":"crossref","first-page":"jcs.147736","DOI":"10.1242\/jcs.147736","article-title":"Dynamics of the Toxoplasma gondii inner membrane complex","author":"DT Ouologuem","year":"2014","journal-title":"J Cell Sci"},{"key":"ppat.1012411.ref038","article-title":"IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome.","volume":"e0304222","author":"PS Back","year":"2023","journal-title":"mBio"},{"key":"ppat.1012411.ref039","doi-asserted-by":"crossref","first-page":"e03622","DOI":"10.1128\/mBio.03622-20","article-title":"Identification and Molecular Dissection of IMC32, a Conserved Toxoplasma Inner Membrane Complex Protein That Is Essential for Parasite Replication.","volume":"12","author":"JA Torres","year":"2021","journal-title":"mBio"},{"key":"ppat.1012411.ref040","doi-asserted-by":"crossref","first-page":"e1011707","DOI":"10.1371\/journal.ppat.1011707","article-title":"Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii.","volume":"19","author":"RR Pasquarelli","year":"2023","journal-title":"PLoS Pathog."},{"key":"ppat.1012411.ref041","doi-asserted-by":"crossref","first-page":"e1007946","DOI":"10.1371\/journal.ppat.1007946","article-title":"Toxoplasma F-box protein 1 is required for daughter cell scaffold function during parasite replication","volume":"15","author":"CG Baptista","year":"2019","journal-title":"PLOS Pathog."},{"key":"ppat.1012411.ref042","doi-asserted-by":"crossref","first-page":"e12302","DOI":"10.1371\/journal.pone.0012302","article-title":"A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis.","volume":"5","author":"A Lorestani","year":"2010","journal-title":"PloS One."},{"key":"ppat.1012411.ref043","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nbt.4201","article-title":"Efficient proximity labeling in living cells and organisms with TurboID","volume":"36","author":"TC Branon","year":"2018","journal-title":"Nat Biotechnol"},{"key":"ppat.1012411.ref044","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/35051615","article-title":"The protein-protein interaction map of Helicobacter pylori","volume":"409","author":"JC Rain","year":"2001","journal-title":"Nature"},{"key":"ppat.1012411.ref045","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1101\/gr.2659105","article-title":"Protein interaction mapping: a Drosophila case study","volume":"15","author":"E Formstecher","year":"2005","journal-title":"Genome Res"},{"key":"ppat.1012411.ref046","doi-asserted-by":"crossref","first-page":"4644","DOI":"10.1038\/s41467-022-32151-0","article-title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.","volume":"13","author":"K Engelberg","year":"2022","journal-title":"Nat Commun"},{"key":"ppat.1012411.ref047","doi-asserted-by":"crossref","first-page":"D898","DOI":"10.1093\/nar\/gkab929","article-title":"VEuPathDB: the eukaryotic pathogen, vector and host bioinformatics resource center","volume":"50","author":"B Amos","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"ppat.1012411.ref048","doi-asserted-by":"crossref","first-page":"28249","DOI":"10.1038\/srep28249","article-title":"GPS-Lipid: a robust tool for the prediction of multiple lipid modification sites.","volume":"6","author":"Y Xie","year":"2016","journal-title":"Sci Rep"},{"key":"ppat.1012411.ref049","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1093\/protein\/gzn039","article-title":"CSS-Palm 2.0: an updated software for palmitoylation sites prediction","volume":"21","author":"J Ren","year":"2008","journal-title":"Protein Eng Des Sel"},{"key":"ppat.1012411.ref050","doi-asserted-by":"crossref","first-page":"2790","DOI":"10.1093\/bioinformatics\/bty1062","article-title":"DeepCoil-a fast and accurate prediction of coiled-coil domains in protein sequences.","volume":"35","author":"J Ludwiczak","year":"2019","journal-title":"Bioinforma Oxf Engl."},{"key":"ppat.1012411.ref051","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1126\/science.252.5009.1162","article-title":"Predicting coiled coils from protein sequences","volume":"252","author":"A Lupas","year":"1991","journal-title":"Science"},{"key":"ppat.1012411.ref052","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/nmeth.1401","article-title":"An auxin-based degron system for the rapid depletion of proteins in nonplant cells","volume":"6","author":"K Nishimura","year":"2009","journal-title":"Nat Methods"},{"key":"ppat.1012411.ref053","doi-asserted-by":"crossref","first-page":"e00375","DOI":"10.1128\/mBio.00375-17","article-title":"Plasma Membrane Association by N-Acylation Governs PKG Function in Toxoplasma gondii.","volume":"8","author":"KM Brown","year":"2017","journal-title":"mBio"},{"key":"ppat.1012411.ref054","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1038\/s41592-019-0512-x","article-title":"An efficient auxin-inducible degron system with low basal degradation in human cells.","volume":"16","author":"S Li","year":"2019","journal-title":"Nat Methods"},{"key":"ppat.1012411.ref055","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1126\/science.1074553","article-title":"Role of Toxoplasma gondii Myosin A in Powering Parasite Gliding and Host Cell Invasion","volume":"298","author":"M. Meissner","year":"2002","journal-title":"Science"},{"key":"ppat.1012411.ref056","first-page":"10","article-title":"RON5 Is Critical for Organization and Function of the Toxoplasma Moving Junction Complex.","author":"JR Beck","year":"2014","journal-title":"PLoS Pathog."},{"key":"ppat.1012411.ref057","doi-asserted-by":"crossref","first-page":"W402","DOI":"10.1093\/nar\/gkz297","article-title":"The PSIPRED Protein Analysis Workbench: 20 years on","volume":"47","author":"DWA Buchan","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"ppat.1012411.ref058","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.chom.2010.09.002","article-title":"Functional dissection of the apicomplexan glideosome molecular architecture","volume":"8","author":"K Fr\u00e9nal","year":"2010","journal-title":"Cell Host Microbe"},{"key":"ppat.1012411.ref059","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/j.bbamcr.2013.02.022","article-title":"N-terminal palmitoylation is required for Toxoplasma gondii HSP20 inner membrane complex localization","volume":"1833","author":"MG De Napoli","year":"2013","journal-title":"Biochim Biophys Acta BBA\u2014Mol Cell Res"},{"key":"ppat.1012411.ref060","first-page":"e2206595","article-title":"Cryo-Electron Tomography of Toxoplasma gondii Indicates That the Conoid Fiber May Be Derived from Microtubules.","volume":"10","author":"Z Li","year":"2023","journal-title":"Adv Sci Weinh Baden-Wurtt Ger"},{"key":"ppat.1012411.ref061","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.chom.2011.09.004","article-title":"The Phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii Reveal Unusual Adaptations Within and Beyond the Parasites\u2019 Boundaries","volume":"10","author":"M Treeck","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ppat.1012411.ref062","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/cmi.12186","article-title":"The Toxoplasma gondii calcium-dependent protein kinase 7 is involved in early steps of parasite division and is crucial for parasite survival","volume":"16","author":"J Morlon-Guyot","year":"2014","journal-title":"Cell Microbiol"},{"key":"ppat.1012411.ref063","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1111\/cmi.12571","article-title":"The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence","volume":"18","author":"L Berry","year":"2016","journal-title":"Cell Microbiol"},{"key":"ppat.1012411.ref064","doi-asserted-by":"crossref","first-page":"e1002948","DOI":"10.1371\/journal.ppat.1002948","article-title":"A unique protein phosphatase with kelch-like domains (PPKL) in Plasmodium modulates ookinete differentiation, motility and invasion.","volume":"8","author":"DS Guttery","year":"2012","journal-title":"PLoS Pathog."},{"key":"ppat.1012411.ref065","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes","volume":"166","author":"SM Sidik","year":"2016","journal-title":"Cell"},{"key":"ppat.1012411.ref066","doi-asserted-by":"crossref","first-page":"11703","DOI":"10.1073\/pnas.90.24.11703","article-title":"Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria","volume":"90","author":"RG Donald","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ppat.1012411.ref067","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1126\/science.8235614","article-title":"Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker","volume":"262","author":"K Kim","year":"1993","journal-title":"Science"},{"key":"ppat.1012411.ref068","doi-asserted-by":"crossref","first-page":"14010","DOI":"10.1074\/jbc.271.24.14010","article-title":"Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene: Use as a selectable marker for stable transformation","volume":"271","author":"RG Donald","year":"1996","journal-title":"J Biol Chem"},{"key":"ppat.1012411.ref069","doi-asserted-by":"crossref","first-page":"5749","DOI":"10.1073\/pnas.92.12.5749","article-title":"Insertional mutagenesis and marker rescue in a protozoan parasite: cloning of the uracil phosphoribosyltransferase locus from Toxoplasma gondii","volume":"92","author":"RG Donald","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ppat.1012411.ref070","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0166-6851(96)02598-4","article-title":"A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei","volume":"77","author":"P Bastin","year":"1996","journal-title":"Mol Biochem Parasitol"},{"key":"ppat.1012411.ref071","first-page":"3610","article-title":"Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product","volume":"5","author":"GI Evan","year":"1985","journal-title":"Mol Cell Biol"},{"key":"ppat.1012411.ref072","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/nmeth.3365","article-title":"High-performance probes for light and electron microscopy.","volume":"12","author":"S Viswanathan","year":"2015","journal-title":"Nat Methods"},{"key":"ppat.1012411.ref073","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.chom.2016.02.006","article-title":"Phosphatidic Acid-Mediated Signaling Regulates Microneme Secretion in Toxoplasma","volume":"19","author":"HE Bullen","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ppat.1012411.ref074","doi-asserted-by":"crossref","first-page":"e100450","DOI":"10.1371\/journal.pone.0100450","article-title":"Efficient Genome Engineering of Toxoplasma gondii Using CRISPR\/Cas9.","volume":"9","author":"SM Sidik","year":"2014","journal-title":"PLoS ONE"},{"key":"ppat.1012411.ref075","doi-asserted-by":"crossref","first-page":"e01114","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR\/CAS9.","volume":"5","author":"B Shen","year":"2014","journal-title":"mBio"},{"key":"ppat.1012411.ref076","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"M-H Huynh","year":"2009","journal-title":"Eukaryot Cell"},{"key":"ppat.1012411.ref077","doi-asserted-by":"crossref","first-page":"34245","DOI":"10.1074\/jbc.M504158200","article-title":"Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii","volume":"280","author":"PJ Bradley","year":"2005","journal-title":"J Biol Chem"},{"key":"ppat.1012411.ref078","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/978-1-4939-9857-9_18","article-title":"Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii","volume":"2071","author":"PJ Bradley","year":"2020","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"ppat.1012411.ref079","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jprot.2015.07.001","article-title":"ProLuCID: An improved SEQUEST-like algorithm with enhanced sensitivity and specificity","volume":"129","author":"T Xu","year":"2015","journal-title":"J Proteomics"},{"key":"ppat.1012411.ref080","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1021\/pr015504q","article-title":"DTASelect and Contrast: Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics","volume":"1","author":"DL Tabb","year":"2002","journal-title":"J Proteome Res"},{"key":"ppat.1012411.ref081","doi-asserted-by":"crossref","first-page":"13.4.1","DOI":"10.1002\/0471250953.bi1304s16","article-title":"Validation of Tandem Mass Spectrometry Database Search Results Using DTASelect.","volume":"16","author":"D, L. Cociorva","year":"2006","journal-title":"Curr Protoc Bioinforma"},{"key":"ppat.1012411.ref082","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ymeth.2006.07.028","article-title":"Analyzing chromatin remodeling complexes using shotgun proteomics and normalized spectral abundance factors","volume":"40","author":"L Florens","year":"2006","journal-title":"Methods"},{"key":"ppat.1012411.ref083","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","article-title":"A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions","volume":"28","author":"K Barylyuk","year":"2020","journal-title":"Cell Host Microbe"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1012411","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T17:45:48Z","timestamp":1722361548000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012411"}},"subtitle":[],"editor":[{"given":"S\u00e9bastien","family":"Besteiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,18]]},"references-count":83,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1012411","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1012411","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,18]]}}}