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The iron-sulfur cluster [Fe-S] biogenesis ISC pathway, localized within the mitochondrion or mitosome of these parasites, is vital for parasite survival and development. Previous work on\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    and\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    provided insights into the mechanisms of [Fe-S] biogenesis within this phylum, while the transporter linking mitochondria-generated [Fe-S] with the cytosolic [Fe-S] assembly (CIA) pathway remained elusive. This critical step is catalyzed by a well-conserved ABC transporter, termed ATM1 in yeast, ATM3 in plants and ABCB7 in mammals. Here, we identify and characterize this transporter in two clinically relevant Apicomplexa. We demonstrate that depletion of\n                    <jats:italic>Tg<\/jats:italic>\n                    ATM1 does not specifically impair mitochondrial metabolism. Instead, proteomic analyses reveal that\n                    <jats:italic>Tg<\/jats:italic>\n                    ATM1 expression levels inversely correlate with the abundance of proteins that participate in the transfer of [Fe-S] to cytosolic proteins at the outer mitochondrial membrane. Further insights into the role of\n                    <jats:italic>Tg<\/jats:italic>\n                    ATM1 are gained through functional complementation with the well-characterized yeast homolog. Biochemical characterization of\n                    <jats:italic>Pf<\/jats:italic>\n                    ATM1 confirms its role as a functional ABC transporter, modulated by oxidized glutathione (GSSG) and [4Fe-4S].\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1012593","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T13:38:17Z","timestamp":1727703497000},"page":"e1012593","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["ATM1, an essential conserved transporter in Apicomplexa, bridges mitochondrial and cytosolic [Fe-S] biogenesis"],"prefix":"10.1371","volume":"20","author":[{"given":"Deepti","family":"Shrivastava","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest","family":"Abboud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jadhav Prasad","family":"Ramchandra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akanksha","family":"Jha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Marq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Animesh","family":"Chaurasia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyan","family":"Mitra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Sadik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Imran","family":"Siddiqi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Soldati-Favre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Kloehn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9378-7786","authenticated-orcid":true,"given":"Saman","family":"Habib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"ppat.1012593.ref001","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S1369-5274(03)00039-0","article-title":"The role of Fe\u2013S proteins in sensing and regulation in bacteria","volume":"6","author":"PJ Kiley","year":"2003","journal-title":"Curr Opin Microbiol"},{"key":"ppat.1012593.ref002","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/nchembio0406-171","article-title":"Iron-sulfur clusters: ever-expanding roles","volume":"2","author":"M. 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