{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:17:47Z","timestamp":1790417867076,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012696","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000}}],"reference-count":74,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T00:00:00Z","timestamp":1731888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Major Project of the Inner Mongolia Autonomous Region of China","award":["2021ZD0013"],"award-info":[{"award-number":["2021ZD0013"]}]},{"DOI":"10.13039\/100017963","name":"Education Department of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2021GG0156"],"award-info":[{"award-number":["2021GG0156"]}],"id":[{"id":"10.13039\/100017963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060800"],"award-info":[{"award-number":["32060800"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Technology Major Project of Hohhot","award":["2023-JieBangGuaShuai"],"award-info":[{"award-number":["2023-JieBangGuaShuai"]}]},{"DOI":"10.13039\/501100010031","name":"Postdoctoral Research Foundation of China","doi-asserted-by":"publisher","award":["2023MD734180"],"award-info":[{"award-number":["2023MD734180"]}],"id":[{"id":"10.13039\/501100010031","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Respiratory infections caused by <jats:italic>Pseudomonas aeruginosa<\/jats:italic> are a major health problem globally. Current treatment for <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> infections relies solely on antibiotics, but the rise of antibiotic-resistant strains necessitates an urgent need for a protective vaccine. Traditional parenteral vaccines, despite employing potent adjuvants aimed at serotype-dependent immunity, often fail to elicit the desired mucosal immune response. Thus, developing vaccines that target both localized mucosal and systemic immune responses represents a promising direction for future research on <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> vaccination. In this study, we explored EPS301, the exopolysaccharide derived from the lung microbiota strain <jats:italic>Lactobacillus plantarum<\/jats:italic> WXD301, which exhibits excellent self-assembly properties, enabling the formation of homogeneous nanoparticles when encapsulating recombinant PcrV of <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic>, designated as EPS301@rPcrV. Notably, the EPS301 vector effectively enhanced antigen adhesion to the nasal and pulmonary mucosal tissues and prolonged antigen retention. Moreover, EPS301@rPcrV provided effective and sustained protection against <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> pneumonia, surpassing the durability achieved with the \"gold standard\" cholera toxin adjuvant. The EPS301-adjuvanted vaccine formulation elicited robust mucosal IgA and Th17\/\u03b3\u03b417 T cell responses, which exceeded those induced by the CTB-adjuvanted vaccination and were sustained for over 112 days. Additionally, Th 17 and \u03b3\u03b4 17 resident memory T cells induced by EPS301@rPcrV were crucial for protection against <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> challenge. Intriguingly, IL-17A knockout mice exhibited lower survival rates, impaired bacterial clearance ability, and exacerbated lung tissue damage upon EPS301 adjuvanted vaccination against <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic>-induced pneumonia, indicating an IL-17A-dependent protective mechanism. In conclusion, our findings provided direct evidence that EPS301@rPcrV mucosal vaccine is a promising candidate for future clinical application against <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic>-induced pulmonary infection.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012696","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T18:36:52Z","timestamp":1731955012000},"page":"e1012696","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mucosal immunization with the lung Lactobacillus-derived amphiphilic exopolysaccharide adjuvanted recombinant vaccine improved protection against P. aeruginosa infection"],"prefix":"10.1371","volume":"20","author":[{"given":"Haochi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouxin","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhe","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingyuan","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanchen","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingmin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3745-3647","authenticated-orcid":true,"given":"Xiao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"issue":"3","key":"ppat.1012696.ref001","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.cyto.2008.07.009","article-title":"Innate 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