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Despite evidence of a pro-inflammatory environment in the genital tract of HIV-infected women, comprehensive investigations into cervical tissue from this region remain limited. Similarly, the consequences of chronic HIV infection on the integrity of the female genital epithelium are poorly understood, despite its importance in HIV transmission and replication. Ectocervical biopsies were obtained from HIV-seropositive (n = 14) and HIV-seronegative (n = 47) female Kenyan sex workers. RNA sequencing and bioimage analysis of epithelial junction proteins (E-cadherin, desmoglein-1, claudin-1, and zonula occludens-1) were conducted, along with CD4 staining. RNA sequencing revealed upregulation of immunoregulatory genes in HIV-seropositive women, primarily associated with heightened T cell activity and interferon signaling, which further correlated with plasma viral load. Transcription factor analysis confirmed the upregulation of pro-inflammatory transcription factors, such as <jats:italic>RELA<\/jats:italic>, <jats:italic>NFKB1<\/jats:italic>, and <jats:italic>IKZF3<\/jats:italic>, which facilitates HIV persistence in T cells. Conversely, genes and pathways associated with epithelial barrier function and structure were downregulated in the context of HIV. Digital bioimage analysis corroborated these findings, revealing significant disruption of various epithelial junction proteins in ectocervical tissues of the HIV-seropositive women. Thus, chronic HIV infection associated with ectocervical inflammation, characterized by induced T cell responses and interferon signaling, coupled with epithelial disruption. These alterations may influence HIV transmission and heighten susceptibility to other sexually transmitted infections. These findings prompt exploration of therapeutic interventions to address HIV-related complications and mitigate the risk of sexually transmitted infection transmission.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012709","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T18:38:08Z","timestamp":1732041488000},"page":"e1012709","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sustained immune activation and impaired epithelial barrier integrity in the ectocervix of women with chronic HIV infection"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0219-3655","authenticated-orcid":true,"given":"Mathias","family":"Franz\u00e9n Boger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyra","family":"Hasselrot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilde","family":"Kaldhusdal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele H. 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Giorgi","year":"1999","journal-title":"J Infect Dis"},{"issue":"1","key":"ppat.1012709.ref005","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/cei.12814","article-title":"Ageing and inflammation in patients with HIV infection","volume":"187","author":"M Nasi","year":"2017","journal-title":"Clin Exp Immunol"},{"issue":"2","key":"ppat.1012709.ref006","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.imlet.2014.01.008","article-title":"Persistent inflammation in HIV infection: established concepts, new perspectives","volume":"161","author":"M Nasi","year":"2014","journal-title":"Immunol Lett"},{"issue":"8","key":"ppat.1012709.ref007","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1097\/QAD.0b013e32835ecb8b","article-title":"Persistent immune activation in chronic HIV infection: do any interventions work","volume":"27","author":"R 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Winandy","year":"1995","journal-title":"Cell"},{"issue":"11","key":"ppat.1012709.ref045","doi-asserted-by":"crossref","first-page":"2584","DOI":"10.1016\/j.immuni.2023.10.002","article-title":"Single-cell epigenetic, transcriptional, and protein profiling of latent and active HIV-1 reservoir revealed that IKZF3 promotes HIV-1 persistence","volume":"56","author":"Y Wei","year":"2023","journal-title":"Immunity"},{"issue":"10","key":"ppat.1012709.ref046","doi-asserted-by":"crossref","first-page":"5577","DOI":"10.1093\/nar\/gkac407","article-title":"Catchet-MS identifies IKZF1-targeting thalidomide analogues as novel HIV-1 latency reversal agents","volume":"50","author":"E Ne","year":"2022","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"ppat.1012709.ref047","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1038\/emboj.2008.24","article-title":"Perturbed desmosomal cadherin expression in grainy head-like 1-null mice","volume":"27","author":"T Wilanowski","year":"2008","journal-title":"EMBO J"},{"issue":"3","key":"ppat.1012709.ref048","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.devcel.2011.12.001","article-title":"ZNF750 Is a p63 Target Gene that Induces KLF4 to Drive Terminal Epidermal Differentiation","volume":"22","author":"GL Sen","year":"2012","journal-title":"Dev Cell"},{"issue":"1","key":"ppat.1012709.ref049","first-page":"1","article-title":"CRISPR\/Cas9-Mediated Deletion of Foxn1 in NOD\/SCID\/IL2rg\u2212\/\u2212 Mice Results in Severe Immunodeficiency","volume":"7","author":"X Wei","year":"2017","journal-title":"Scientific Reports 2017 7:1"},{"issue":"7","key":"ppat.1012709.ref050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004251","article-title":"T-bet and Eomes Are Differentially Linked to the Exhausted Phenotype of CD8+ T Cells in HIV Infection","volume":"10","author":"M Buggert","year":"2014","journal-title":"PLoS 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Tugizov","year":"2016","journal-title":"Tissue Barriers"},{"issue":"6","key":"ppat.1012709.ref065","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1111\/vde.12956","article-title":"Grainyhead-like transcription factors: guardians of the skin barrier","volume":"32","author":"Z Deng","year":"2021","journal-title":"Vet Dermatol."},{"issue":"3","key":"ppat.1012709.ref066","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.devcel.2011.12.001","article-title":"ZNF750 is a p63 Target Gene that Induces KLF4 to Drive Terminal Epidermal Differentiation","volume":"22","author":"GL Sen","year":"2012","journal-title":"Dev Cell"},{"issue":"12","key":"ppat.1012709.ref067","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0226343","article-title":"HIV-1 proteins gp120 and tat induce the epithelial\u2013mesenchymal transition in oral and genital mucosal epithelial cells.","volume":"14","author":"K Lien","year":"2019","journal-title":"PLoS One."},{"issue":"2","key":"ppat.1012709.ref068","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0088803","article-title":"HIV-associated disruption of tight and adherens junctions of oral epithelial cells facilitates HSV-1 infection and spread.","volume":"9","author":"I Sufiawati","year":"2014","journal-title":"PLoS One"},{"issue":"6","key":"ppat.1012709.ref069","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1084\/jem.20041196","article-title":"Primary HIV-1 Infection Is Associated with Preferential Depletion of CD4+ T Lymphocytes from Effector Sites in the Gastrointestinal Tract","volume":"200","author":"S Mehandru","year":"2004","journal-title":"J Exp Med"},{"issue":"1","key":"ppat.1012709.ref070","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/mi.2016.30","article-title":"MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation","volume":"10","author":"A Gibbs","year":"2017","journal-title":"Mucosal Immunol"},{"issue":"3","key":"ppat.1012709.ref071","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1097\/QAI.0b013e3181a74f89","article-title":"Abundant and superficial expression of C-type lectin receptors in ectocervix of women at risk of HIV infection.","volume":"51","author":"T Hirbod","year":"2009","journal-title":"J Acquir Immune Defic Syndr."},{"issue":"1","key":"ppat.1012709.ref072","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-019-12732-2","article-title":"Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa.","volume":"10","author":"J Cantero-P\u00e9rez","year":"2019","journal-title":"Nat Commun."},{"issue":"1","key":"ppat.1012709.ref073","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"MD Robinson","year":"2010","journal-title":"Bioinformatics"},{"issue":"3","key":"ppat.1012709.ref074","doi-asserted-by":"crossref","first-page":"e90","DOI":"10.1002\/cpz1.90","article-title":"Gene Set Knowledge Discovery with Enrichr.","volume":"1","author":"Z Xie","year":"2021","journal-title":"Curr Protoc"},{"key":"ppat.1012709.ref075","unstructured":"An R interface to the Enrichr database. [cited 2024 Sep 27]. Available from: https:\/\/cran.r-project.org\/web\/packages\/enrichR\/vignettes\/enrichR.html."},{"key":"ppat.1012709.ref076","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1186\/1471-2105-14-128","article-title":"Enrichr: interactive and collaborative HTML5 gene list enrichment analysis tool","volume":"14","author":"EY Chen","year":"2013","journal-title":"BMC Bioinformatics"},{"key":"ppat.1012709.ref077","doi-asserted-by":"crossref","first-page":"W90","DOI":"10.1093\/nar\/gkw377","article-title":"Enrichr: a comprehensive gene set enrichment analysis web server 2016 update","volume":"44","author":"M V. Kuleshov","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"ppat.1012709.ref078","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1093\/infdis\/jiaa514","article-title":"Regular Use of Depot Medroxyprogesterone Acetate Causes Thinning of the Superficial Lining and Apical Distribution of Human Immunodeficiency Virus Target Cells in the Human Ectocervix","volume":"225","author":"G Edfeldt","year":"2022","journal-title":"J Infect Dis"},{"issue":"7","key":"ppat.1012709.ref079","first-page":"676","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nature Methods 2012 9:7"},{"issue":"5","key":"ppat.1012709.ref080","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.1109\/TIP.2012.2185938","article-title":"Contrast-independent curvilinear structure detection in biomedical images","volume":"21","author":"B Obara","year":"2012","journal-title":"IEEE Transactions on Image Processing"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1012709","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T18:43:29Z","timestamp":1733251409000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012709"}},"subtitle":[],"editor":[{"given":"Gilda","family":"Tachedjian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,11,19]]},"references-count":80,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1012709","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1012709","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,19]]}}}