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N6-methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) is a prevalent internal chemical modification in eukaryotic mRNA that has been reported to regulate viral infection. However, the role of m\n                    <jats:sup>6<\/jats:sup>\n                    A modification during SFTSV infection remains elusive. We here reported that SFTSV RNAs bear m\n                    <jats:sup>6<\/jats:sup>\n                    A modification during infection. Manipulating the expressions or activities of host m\n                    <jats:sup>6<\/jats:sup>\n                    A regulators significantly impacted SFTSV infection. Mechanistically, SFTSV recruited m\n                    <jats:sup>6<\/jats:sup>\n                    A regulators through the nucleoprotein to modulate the m\n                    <jats:sup>6<\/jats:sup>\n                    A modification of viral RNA, eventually resulting in enhanced infection by promoting viral mRNA translation efficiency and\/or genome RNA stability. m\n                    <jats:sup>6<\/jats:sup>\n                    A mutations in the S genome diminished virus particle production, while m\n                    <jats:sup>6<\/jats:sup>\n                    A mutations in the G transcript impaired the replication of recombinant vesicular stomatitis virus (rVSV) expressing G protein\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Interestingly, m\n                    <jats:sup>6<\/jats:sup>\n                    A modification was evolutionarily conserved and facilitated SFTSV infection in primary tick cells. These findings may open an avenue for the development of m\n                    <jats:sup>6<\/jats:sup>\n                    A-targeted anti-SFTSV vaccines, drugs, and innovative strategies for the prevention and control of tick-borne disease.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1012725","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T13:49:07Z","timestamp":1732542547000},"page":"e1012725","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["N6-methyladenosine RNA modification promotes Severe Fever with Thrombocytopenia Syndrome Virus infection"],"prefix":"10.1371","volume":"20","author":[{"given":"Zhiqiang","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1871-8390","authenticated-orcid":true,"given":"Jianfeng","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"issue":"16","key":"ppat.1012725.ref001","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1056\/NEJMoa1010095","article-title":"Fever 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Kobayashi","year":"2020","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"ppat.1012725.ref005","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3201\/eid2505.181463","article-title":"Endemic Severe Fever with Thrombocytopenia Syndrome, Vietnam","volume":"25","author":"XC Tran","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"ppat.1012725.ref006","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/s41541-020-00257-5","article-title":"Baseline mapping of severe fever with thrombocytopenia syndrome virology, epidemiology and vaccine research and development.","volume":"5","author":"NE Bopp","year":"2020","journal-title":"NPJ Vaccines."},{"issue":"9","key":"ppat.1012725.ref007","doi-asserted-by":"crossref","DOI":"10.3201\/eid2409.171427","article-title":"World Health Organization Methodology to Prioritize Emerging Infectious Diseases in Need of Research and Development","volume":"24","author":"MS Mehand","year":"2018","journal-title":"Emerg 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Furuse","year":"2021","journal-title":"Int J Mol Sci"},{"issue":"1","key":"ppat.1012725.ref015","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s12943-020-01263-w","article-title":"The role of m(6)A, m(5)C and Psi RNA modifications in cancer: Novel therapeutic opportunities.","volume":"20","author":"P Nombela","year":"2021","journal-title":"Mol Cancer"},{"issue":"10","key":"ppat.1012725.ref016","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/s41579-020-0382-3","article-title":"Epigenetic and epitranscriptomic regulation of viral replication","volume":"18","author":"K Tsai","year":"2020","journal-title":"Nature Reviews Microbiology"},{"issue":"2","key":"ppat.1012725.ref017","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nchembio.1432","article-title":"A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation","volume":"10","author":"J Liu","year":"2014","journal-title":"Nat Chem 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Roundtree","year":"2017","journal-title":"Cell"},{"issue":"9","key":"ppat.1012725.ref045","doi-asserted-by":"crossref","first-page":"R137","DOI":"10.1186\/gb-2008-9-9-r137","article-title":"Model-based analysis of ChIP-Seq (MACS).","volume":"9","author":"Y Zhang","year":"2008","journal-title":"Genome Biol"},{"issue":"14","key":"ppat.1012725.ref046","doi-asserted-by":"crossref","first-page":"e0042921","DOI":"10.1128\/JVI.00429-21","article-title":"Characterization of the Molecular Interactions That Govern the Packaging of Viral RNA Segments into Rift Valley Fever Phlebovirus Particles","volume":"95","author":"B Tercero","year":"2021","journal-title":"J Virol"},{"key":"ppat.1012725.ref047","doi-asserted-by":"crossref","first-page":"1132757","DOI":"10.3389\/fcimb.2023.1132757","article-title":"Mechanistic insight into the efficient packaging of antigenomic S RNA into Rift Valley fever virus particles","volume":"13","author":"B Tercero","year":"2023","journal-title":"Front Cell Infect 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Li","year":"2020","journal-title":"Cell Rep"},{"issue":"2","key":"ppat.1012725.ref054","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.molcel.2016.05.041","article-title":"Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases.","volume":"63","author":"P Wang","year":"2016","journal-title":"Mol Cell"},{"issue":"4","key":"ppat.1012725.ref055","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s13238-018-0518-7","article-title":"Solution structure of the RNA recognition domain of METTL3-METTL14 N(6)-methyladenosine methyltransferase.","volume":"10","author":"J Huang","year":"2019","journal-title":"Protein Cell."},{"issue":"6","key":"ppat.1012725.ref056","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/s13238-013-3901-4","article-title":"The nucleoprotein of severe fever with thrombocytopenia syndrome virus processes a stable hexameric ring to facilitate RNA encapsidation","volume":"4","author":"H Zhou","year":"2013","journal-title":"Protein Cell"},{"issue":"8","key":"ppat.1012725.ref057","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1038\/s41556-022-00968-y","article-title":"METTL3 preferentially enhances non-m(6)A translation of epigenetic factors and promotes tumourigenesis.","volume":"24","author":"X Wei","year":"2022","journal-title":"Nat Cell Biol"},{"issue":"3","key":"ppat.1012725.ref058","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.molcel.2016.03.021","article-title":"The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.","volume":"62","author":"S Lin","year":"2016","journal-title":"Mol Cell"},{"issue":"1","key":"ppat.1012725.ref059","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1038\/s41467-020-16306-5","article-title":"N(6)-methyladenosine regulates glycolysis of cancer cells through PDK4.","volume":"11","author":"Z Li","year":"2020","journal-title":"Nat 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Ther."},{"issue":"3","key":"ppat.1012725.ref066","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.virs.2022.02.008","article-title":"A single dose of recombinant VSV-RABV(G) vaccine provides full protection against RABV challenge","volume":"37","author":"M Liang","year":"2022","journal-title":"Virol Sin"},{"key":"ppat.1012725.ref067","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41541-018-0096-y","article-title":"Single dose of a rVSV-based vaccine elicits complete protection against severe fever with thrombocytopenia syndrome virus","volume":"4","author":"F Dong","year":"2019","journal-title":"NPJ Vaccines"},{"issue":"10","key":"ppat.1012725.ref068","doi-asserted-by":"crossref","first-page":"4477","DOI":"10.1073\/pnas.92.10.4477","article-title":"Recombinant vesicular stomatitis viruses from DNA","volume":"92","author":"ND Lawson","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"ppat.1012725.ref069","article-title":"Two Point Mutations in the Glycoprotein of SFTSV Enhance the Propagation Recombinant Vesicular Stomatitis Virus Vectors at Assembly Step.","volume":"15","author":"Q Hu","year":"2023","journal-title":"Viruses."},{"issue":"16","key":"ppat.1012725.ref070","doi-asserted-by":"crossref","first-page":"5327","DOI":"10.1093\/nar\/gkq266","article-title":"Yeast targets for mRNA methylation","volume":"38","author":"Z Bodi","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"ppat.1012725.ref071","article-title":"The PyMOL Molecular Graphics System, Version 2.5.","author":"LLC Schrodinger","year":"2021"},{"issue":"6","key":"ppat.1012725.ref072","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/s41592-022-01488-1","article-title":"ColabFold: making protein folding accessible to all.","volume":"19","author":"M Mirdita","year":"2022","journal-title":"Nat Methods."},{"issue":"6","key":"ppat.1012725.ref073","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1038\/s41564-020-0712-2","article-title":"Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation","volume":"5","author":"P Wang","year":"2020","journal-title":"Nat Microbiol"},{"issue":"26","key":"ppat.1012725.ref074","doi-asserted-by":"crossref","first-page":"7154","DOI":"10.1073\/pnas.1603827113","article-title":"Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion","volume":"113","author":"S Halldorsson","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"ppat.1012725.ref075","doi-asserted-by":"crossref","DOI":"10.3390\/v13050876","article-title":"The Role of Non-Structural Protein NSs in the Pathogenesis of Severe Fever with Thrombocytopenia Syndrome.","volume":"13","author":"J Khalil","year":"2021","journal-title":"Viruses."},{"issue":"10","key":"ppat.1012725.ref076","doi-asserted-by":"crossref","first-page":"5684","DOI":"10.1093\/nar\/gkaa269","article-title":"Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein","volume":"48","author":"J Li","year":"2020","journal-title":"Nucleic Acids Res"},{"issue":"19","key":"ppat.1012725.ref077","doi-asserted-by":"crossref","first-page":"2001402","DOI":"10.1002\/advs.202001402","article-title":"TRADES: Targeted RNA Demethylation by SunTag System.","volume":"7","author":"J Mo","year":"2020","journal-title":"Adv Sci (Weinh)."},{"issue":"2","key":"ppat.1012725.ref078","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/0042-6822(78)90191-5","article-title":"3-Deazaadenosine, an inhibitor of adenosylhomocysteine hydrolase, inhibits reproduction of Rous sarcoma virus and transformation of chick embryo cells","volume":"89","author":"JP Bader","year":"1978","journal-title":"Virology"},{"issue":"2","key":"ppat.1012725.ref079","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0042-6822(90)90517-U","article-title":"Specific inhibition of the synthesis of influenza virus late proteins and stimulation of early, M2, and NS2 protein synthesis by 3-deazaadenosine","volume":"177","author":"AA Fischer","year":"1990","journal-title":"Virology"},{"issue":"8790","key":"ppat.1012725.ref080","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/0140-6736(92)90133-N","article-title":"3-Deaza-adenosine and inhibition of HIV","volume":"339","author":"CW Flexner","year":"1992","journal-title":"Lancet"},{"issue":"4\u20135","key":"ppat.1012725.ref081","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0166-3542(90)90003-P","article-title":"Evaluation of the toxicity and antiviral activity of carbocyclic 3-deazaadenosine against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats","volume":"14","author":"PR Wyde","year":"1990","journal-title":"Antiviral Res"},{"issue":"2","key":"ppat.1012725.ref082","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0166-3542(00)00066-8","article-title":"Treatment of lethal Ebola virus infection in mice with a single dose of an S-adenosyl-L-homocysteine hydrolase inhibitor","volume":"45","author":"M Bray","year":"2000","journal-title":"Antiviral Res"},{"issue":"13\u201314","key":"ppat.1012725.ref083","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1101\/gad.348320.121","article-title":"Targeting the m(6)A RNA modification pathway blocks SARS-CoV-2 and HCoV-OC43 replication","volume":"35","author":"HM Burgess","year":"2021","journal-title":"Genes Dev"},{"issue":"5","key":"ppat.1012725.ref084","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1016\/j.chom.2016.10.002","article-title":"Dynamics of Human and Viral RNA Methylation during Zika Virus Infection","volume":"20","author":"G Lichinchi","year":"2016","journal-title":"Cell Host Microbe"},{"issue":"4","key":"ppat.1012725.ref085","doi-asserted-by":"crossref","first-page":"e0106721","DOI":"10.1128\/mBio.01067-21","article-title":"Methyltransferase-like 3 Modulates Severe Acute Respiratory Syndrome Coronavirus-2 RNA N6-Methyladenosine Modification and Replication.","volume":"12","author":"X Zhang","year":"2021","journal-title":"mBio."},{"issue":"7574","key":"ppat.1012725.ref086","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/nature15377","article-title":"Dynamic m(6)A mRNA methylation directs translational control of heat shock response.","volume":"526","author":"J Zhou","year":"2015","journal-title":"Nature"},{"issue":"4","key":"ppat.1012725.ref087","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1261\/rna.064063.117","article-title":"Interactions, localization, and phosphorylation of the m(6)A generating METTL3-METTL14-WTAP complex","volume":"24","author":"E Scholler","year":"2018","journal-title":"RNA"},{"issue":"1","key":"ppat.1012725.ref088","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s12943-021-01447-y","article-title":"METTL3\/IGF2BP3 axis inhibits tumor immune surveillance by upregulating N(6)-methyladenosine modification of PD-L1 mRNA in breast cancer.","volume":"21","author":"W Wan","year":"2022","journal-title":"Mol Cancer"},{"issue":"7","key":"ppat.1012725.ref089","doi-asserted-by":"crossref","first-page":"110373","DOI":"10.1016\/j.celrep.2022.110373","article-title":"TBK1-METTL3 axis facilitates antiviral immunity","volume":"38","author":"J Chen","year":"2022","journal-title":"Cell Rep"},{"issue":"14","key":"ppat.1012725.ref090","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.1038\/sj.emboj.7601765","article-title":"Reconstitution reveals the functional core of mammalian eIF3","volume":"26","author":"M Masutani","year":"2007","journal-title":"EMBO J"},{"issue":"1","key":"ppat.1012725.ref091","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1186\/s13045-021-01204-0","article-title":"N6-methyladenosine reader IMP2 stabilizes the ZFAS1\/OLA1 axis and activates the Warburg effect: implication in colorectal cancer","volume":"14","author":"S Lu","year":"2021","journal-title":"J Hematol Oncol"},{"issue":"Pt 3","key":"ppat.1012725.ref092","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.semcancer.2022.05.009","article-title":"The IGF2BP family of RNA binding proteins links epitranscriptomics to cancer","volume":"86","author":"D Ramesh-Kumar","year":"2022","journal-title":"Semin Cancer Biol"},{"issue":"36","key":"ppat.1012725.ref093","first-page":"E7564","article-title":"Structures of phlebovirus glycoprotein Gn and identification of a neutralizing antibody epitope","volume":"114","author":"Y Wu","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"ppat.1012725.ref094","doi-asserted-by":"crossref","first-page":"e1006316","DOI":"10.1371\/journal.ppat.1006316","article-title":"A role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry.","volume":"13","author":"MJ Drake","year":"2017","journal-title":"PLoS Pathog."},{"issue":"6633","key":"ppat.1012725.ref095","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1126\/science.abj9090","article-title":"Exon architecture controls mRNA m(6)A suppression and gene expression.","volume":"379","author":"PC He","year":"2023","journal-title":"Science"},{"issue":"24","key":"ppat.1012725.ref096","doi-asserted-by":"crossref","first-page":"6860","DOI":"10.1093\/emboj\/19.24.6860","article-title":"The spliceosome deposits multiple proteins 20\u201324 nucleotides upstream of mRNA exon-exon junctions","volume":"19","author":"H Le Hir","year":"2000","journal-title":"EMBO J"},{"issue":"10","key":"ppat.1012725.ref097","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.3201\/eid2110.150126","article-title":"Haemaphysalis longicornis Ticks as Reservoir and Vector of Severe Fever with Thrombocytopenia Syndrome Virus in China","volume":"21","author":"LM Luo","year":"2015","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"ppat.1012725.ref098","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1016\/j.cell.2020.07.023","article-title":"Large-Scale Comparative Analyses of Tick Genomes Elucidate Their Genetic Diversity and Vector Capacities","volume":"182","author":"N Jia","year":"2020","journal-title":"Cell"},{"issue":"1","key":"ppat.1012725.ref099","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/s42003-022-03566-8","article-title":"N(6)-methyladenosine modification of the Aedes aegypti transcriptome and its alteration upon dengue virus infection in Aag2 cell line.","volume":"5","author":"Z Dai","year":"2022","journal-title":"Commun Biol"},{"key":"ppat.1012725.ref100","article-title":"Targeting Haemaphysalis longicornis serpin to prevent tick feeding and pathogen transmission.","author":"T Feng","year":"2023","journal-title":"Insect Sci."},{"issue":"6","key":"ppat.1012725.ref101","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms10061086","article-title":"New Cell Lines Derived from European Tick Species.","volume":"10","author":"L Bell-Sakyi","year":"2022","journal-title":"Microorganisms"},{"issue":"9","key":"ppat.1012725.ref102","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1038\/s41423-019-0264-7","article-title":"A novel oncolytic virus engineered with PD-L1 scFv effectively inhibits tumor growth in a mouse model","volume":"16","author":"C Wu","year":"2019","journal-title":"Cell Mol Immunol"},{"key":"ppat.1012725.ref103","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/B978-0-12-418687-3.00018-5","article-title":"Transient mammalian cell transfection with polyethylenimine (PEI).","volume":"529","author":"PA Longo","year":"2013","journal-title":"Methods Enzymol"},{"key":"ppat.1012725.ref104","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-1-4939-6807-7_3","article-title":"Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation.","volume":"1562","author":"BF Yuan","year":"2017","journal-title":"Methods Mol Biol"},{"issue":"1","key":"ppat.1012725.ref105","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/nprot.2012.148","article-title":"Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing.","volume":"8","author":"D Dominissini","year":"2013","journal-title":"Nat Protoc"},{"key":"ppat.1012725.ref106","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/978-1-4939-8808-2_16","article-title":"Profiling of N(6)-Methyladenosine in Zika Virus RNA and Host Cellular mRNA.","volume":"1870","author":"G Lichinchi","year":"2019","journal-title":"Methods Mol Biol"},{"issue":"8","key":"ppat.1012725.ref107","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1038\/s41588-021-00890-3","article-title":"Genetic drivers of m(6)A methylation in human brain, lung, heart and muscle.","volume":"53","author":"X Xiong","year":"2021","journal-title":"Nat Genet"},{"issue":"1","key":"ppat.1012725.ref108","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s13059-020-02249-z","article-title":"N6-methyladenosine dynamics in neurodevelopment and aging, and its potential role in Alzheimer\u2019s disease","volume":"22","author":"AM Shafik","year":"2021","journal-title":"Genome Biol"},{"issue":"3","key":"ppat.1012725.ref109","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"B Langmead","year":"2009","journal-title":"Genome Biol"},{"issue":"8","key":"ppat.1012725.ref110","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype","volume":"37","author":"D Kim","year":"2019","journal-title":"Nat Biotechnol"},{"issue":"1","key":"ppat.1012725.ref111","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer","volume":"29","author":"JT Robinson","year":"2011","journal-title":"Nat Biotechnol"},{"key":"ppat.1012725.ref112","doi-asserted-by":"crossref","first-page":"113701","DOI":"10.1016\/j.jviromet.2019.113701","article-title":"A strand-specific real-time quantitative RT-PCR assay for distinguishing the genomic and antigenomic RNAs of Rift Valley fever phlebovirus","volume":"272","author":"B Tercero","year":"2019","journal-title":"J Virol Methods"},{"issue":"8","key":"ppat.1012725.ref113","doi-asserted-by":"crossref","first-page":"e52205","DOI":"10.15252\/embr.202052205","article-title":"RNA-binding protein RBM47 stabilizes IFNAR1 mRNA to potentiate host antiviral activity","volume":"22","author":"K Wang","year":"2021","journal-title":"EMBO Rep"},{"issue":"1","key":"ppat.1012725.ref114","doi-asserted-by":"crossref","first-page":"e92","DOI":"10.1002\/cpmc.92","article-title":"Rift Valley Fever Virus: Propagation, Quantification, and Storage.","volume":"55","author":"MR Smith","year":"2019","journal-title":"Curr Protoc Microbiol."},{"key":"ppat.1012725.ref115","article-title":"A simple method of estimating fifty per cent endpoints.","author":"LJ Reed","year":"1938"},{"key":"ppat.1012725.ref116","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0076-6879(08)02617-7","article-title":"Messenger RNA half-life measurements in mammalian cells","volume":"448","author":"CY Chen","year":"2008","journal-title":"Methods Enzymol"},{"issue":"D1","key":"ppat.1012725.ref117","doi-asserted-by":"crossref","first-page":"D523","DOI":"10.1093\/nar\/gkac1052","article-title":"UniProt: the Universal Protein Knowledgebase in 2023","volume":"51","author":"C. UniProt","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"ppat.1012725.ref118","doi-asserted-by":"crossref","first-page":"W5","DOI":"10.1093\/nar\/gkn201","article-title":"NCBI BLAST: a better web interface","volume":"36","author":"M Johnson","year":"2008","journal-title":"Nucleic Acids Res"},{"issue":"W1","key":"ppat.1012725.ref119","doi-asserted-by":"crossref","first-page":"W276","DOI":"10.1093\/nar\/gkac240","article-title":"Search and sequence analysis tools services from EMBL-EBI in 2022","volume":"50","author":"F Madeira","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"ppat.1012725.ref120","first-page":"42","article-title":"Deciphering key features in protein structures with the new ENDscript server","author":"X Robert","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"7873","key":"ppat.1012725.ref121","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"J Jumper","year":"2021","journal-title":"Nature"},{"issue":"D1","key":"ppat.1012725.ref122","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"M Varadi","year":"2022","journal-title":"Nucleic Acids Res"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1012725","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T00:00:00Z","timestamp":1733702400000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012725","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T13:43:51Z","timestamp":1733751831000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012725"}},"subtitle":[],"editor":[{"given":"Holly","family":"Ramage","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,11,25]]},"references-count":122,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1012725","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,25]]}}}