{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:12:01Z","timestamp":1790878321569,"version":"4.1.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T00:00:00Z","timestamp":1733443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS121294"],"award-info":[{"award-number":["R01NS121294"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["Supplement to R01NS121294"],"award-info":[{"award-number":["Supplement to R01NS121294"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R21NS127002"],"award-info":[{"award-number":["R21NS127002"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013647","name":"College of Natural Sciences, University of Massachusetts Amherst","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013647","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012417","name":"College of Veterinary Medicine and Biomedical Sciences, Colorado State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012417","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    In patients with synucleinopathies, the protein \u03b1-synuclein misfolds into multiple conformations, each of which determines whether a patient develops multiple system atrophy (MSA) or one of three Lewy body diseases (LBDs). However, patients may also first present with pure autonomic failure, which strictly impacts autonomic nerves in the periphery, which can then phenoconvert into MSA or a LBD. When neuroinvasion happens, it remains unknown if strain properties are retained or if strain adaptation occurs, even though neuroinvasion of some prion protein (PrP) strains is known to result in the emergence of novel PrP strain variants. To investigate this question in synucleinopathies, we inoculated TgM83\n                    <jats:sup>+\/-<\/jats:sup>\n                    mice, which express human \u03b1-synuclein with the A53T mutation, with a mouse-passaged MSA patient sample either intracranially (i.c.) or into the sciatic nerve (sc.n.), and compared the biochemical and biological properties of \u03b1-synuclein prions in the brains of terminal mice. Importantly, while i.c. and sc.n. transmission studies generated pathogenic \u03b1-synuclein with similar properties, both the primary and secondary passaged MSA samples had different infectivity profiles in a panel of \u03b1-syn140-YFP cells than the starting MSA patient sample, indicating that MSA prions adapt during initial passage in TgM83\n                    <jats:sup>+\/-<\/jats:sup>\n                    mice. Similarly, using i.c. inoculation of A53T preformed fibrils to study strain selection, we found both biochemical and biological evidence that mouse passage exerts a selective pressure on \u03b1-synuclein prions in which a sub-population of starting conformations emerges in terminal animals. Together, these findings demonstrate that similar conformational selective pressures known to impact PrP prion replication also impact replication of \u03b1-synuclein prions.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1012746","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T13:21:49Z","timestamp":1733491309000},"page":"e1012746","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["A-synuclein prion strains differentially adapt after passage in mice"],"prefix":"10.1371","volume":"20","author":[{"given":"Sara A. M.","family":"Holec","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chase R.","family":"Khedmatgozar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelbe J.","family":"Schure","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Pham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4029-1968","authenticated-orcid":true,"given":"Amanda L.","family":"Woerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,6]]},"reference":[{"issue":"7825","key":"ppat.1012746.ref001","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/s41586-020-2317-6","article-title":"Structures of \u03b1-synuclein filaments from multiple system atrophy","volume":"585","author":"M Schweighauser","year":"2020","journal-title":"Nature"},{"issue":"7933","key":"ppat.1012746.ref002","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/s41586-022-05319-3","article-title":"Structures of alpha-synuclein filaments from human brains with Lewy pathology","volume":"610","author":"Y Yang","year":"2022","journal-title":"Nature"},{"key":"ppat.1012746.ref003","article-title":"Structure of a novel \u03b1-synuclein filament fold from multiple system atrophy","author":"NL Yan","year":"2024","journal-title":"bioRxiv"},{"issue":"1","key":"ppat.1012746.ref004","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41593-019-0541-x","article-title":"alpha-Synuclein strains target distinct brain regions and cell types","volume":"23","author":"A Lau","year":"2020","journal-title":"Nat Neurosci"},{"issue":"1","key":"ppat.1012746.ref005","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1038\/s41531-021-00264-w","article-title":"Similar neuronal imprint and no cross-seeded fibrils in \u03b1-synuclein aggregates from MSA and Parkinson\u2019s disease","volume":"8","author":"F Laferri\u00e8re","year":"2022","journal-title":"NPJ Parkinsons Dis"},{"issue":"1","key":"ppat.1012746.ref006","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s00401-017-1762-2","article-title":"MSA prions exhibit remarkable stability and resistance to inactivation","volume":"135","author":"AL Woerman","year":"2018","journal-title":"Acta Neuropathol"},{"issue":"12","key":"ppat.1012746.ref007","doi-asserted-by":"crossref","first-page":"e1010956","DOI":"10.1371\/journal.ppat.1010956","article-title":"The E46K mutation modulates \u03b1-synuclein prion replication in transgenic mice","volume":"18","author":"SAM Holec","year":"2022","journal-title":"PLoS Pathog"},{"issue":"2","key":"ppat.1012746.ref008","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1073\/pnas.1719369115","article-title":"Familial Parkinson\u2019s point mutation abolishes multiple system atrophy prion replication","volume":"115","author":"AL Woerman","year":"2018","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"ppat.1012746.ref009","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s00401-022-02476-7","article-title":"Multiple system atrophy prions transmit neurological disease to mice expressing wild-type human \u03b1-synuclein","volume":"144","author":"SAM Holec","year":"2022","journal-title":"Acta Neuropathol"},{"issue":"38","key":"ppat.1012746.ref010","doi-asserted-by":"crossref","first-page":"E5308","DOI":"10.1073\/pnas.1514475112","article-title":"Evidence for \u03b1-synuclein prions causing multiple system atrophy in humans with parkinsonism","volume":"112","author":"SB Prusiner","year":"2015","journal-title":"Proc Natl Acad Sci USA"},{"issue":"7706","key":"ppat.1012746.ref011","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41586-018-0104-4","article-title":"Cellular milieu imparts distinct pathological \u03b1-synuclein strains in \u03b1-synucleinopathies","volume":"557","author":"C Peng","year":"2018","journal-title":"Nature"},{"issue":"3","key":"ppat.1012746.ref012","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1074\/jbc.RA118.004471","article-title":"Parkinson\u2019s disease and multiple system atrophy have distinct \u03b1-synuclein seed characteristics","volume":"294","author":"TR Yamasaki","year":"2019","journal-title":"J Biol Chem"},{"issue":"6","key":"ppat.1012746.ref013","doi-asserted-by":"crossref","first-page":"e211349119","DOI":"10.1073\/pnas.2113489119","article-title":"Different \u03b1-synuclein prion strains cause dementia with Lewy bodies and multiple system atrophy","volume":"119","author":"JI Ayers","year":"2022","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7794","key":"ppat.1012746.ref014","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/s41586-020-1984-7","article-title":"Discriminating alpha-synuclein strains in Parkinson\u2019s disease and multiple system atrophy","volume":"578","author":"M Shahnawaz","year":"2020","journal-title":"Nature"},{"issue":"48","key":"ppat.1012746.ref015","doi-asserted-by":"crossref","first-page":"19555","DOI":"10.1073\/pnas.1318268110","article-title":"Transmission of multiple system atrophy prions to transgenic mice","volume":"110","author":"JC Watts","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"issue":"35","key":"ppat.1012746.ref016","doi-asserted-by":"crossref","first-page":"E4949","DOI":"10.1073\/pnas.1513426112","article-title":"Propagation of prions causing synucleinopathies in cultured cells","volume":"112","author":"AL Woerman","year":"2015","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"ppat.1012746.ref017","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s00401-021-02312-4","article-title":"Transmissible \u03b1-synuclein seeding activity in brain and stomach of patients with Parkinson\u2019s disease","volume":"141","author":"A Thomzig","year":"2021","journal-title":"Acta Neuropathol"},{"issue":"1","key":"ppat.1012746.ref018","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s40478-023-01570-5","article-title":"The G51D SNCA mutation generates a slowly progressive \u03b1-synuclein strain in early-onset Parkinson\u2019s disease","volume":"11","author":"HHC Lau","year":"2023","journal-title":"Acta Neuropathol Commun"},{"issue":"1","key":"ppat.1012746.ref019","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0002-8703(27)90177-X","article-title":"Postural hypotension","volume":"3","author":"S Bradbury","year":"1927","journal-title":"American Heart Journal"},{"issue":"12","key":"ppat.1012746.ref020","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1212\/WNL.0000000000003737","article-title":"Pure autonomic failure: Predictors of conversion to clinical CNS involvement","volume":"88","author":"W Singer","year":"2017","journal-title":"Neurology"},{"issue":"6","key":"ppat.1012746.ref021","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1212\/01.WNL.0000138500.73671.DC","article-title":"Autonomic failure as the initial presentation of Parkinson disease and dementia with Lewy bodies","volume":"63","author":"H Kaufmann","year":"2004","journal-title":"Neurology"},{"issue":"4","key":"ppat.1012746.ref022","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1093\/brain\/90.4.725","article-title":"Defective autonomic control of blood vessels in idiopathic orthostatic hypotension","volume":"90","author":"R Bannister","year":"1967","journal-title":"Brain"},{"key":"ppat.1012746.ref023","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1001\/archneur.1960.03840110025004","article-title":"A neurological syndrome associated with orthostatic hypotension: a clinical-pathologic study","volume":"2","author":"GM Shy","year":"1960","journal-title":"Arch Neurol"},{"key":"ppat.1012746.ref024","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1001\/archneur.1963.00460020104008","article-title":"Neurologic manifestations in idiopathic orthostatic hypotension","volume":"8","author":"JE Thomas","year":"1963","journal-title":"Arch Neurol"},{"issue":"7","key":"ppat.1012746.ref025","first-page":"e889","article-title":"Predicting phenoconversion in pure autonomic failure","volume":"95","author":"EA Coon","year":"2020","journal-title":"Neurology"},{"key":"ppat.1012746.ref026","doi-asserted-by":"crossref","first-page":"11858","DOI":"10.1128\/JVI.79.18.11858-11863.2005","article-title":"Extraneural prion neuroinvasion without lymphoreticular system infection","volume":"79","author":"JC Bartz","year":"2005","journal-title":"J Virol"},{"issue":"7","key":"ppat.1012746.ref027","doi-asserted-by":"crossref","first-page":"e1012370","DOI":"10.1371\/journal.ppat.1012370","article-title":"Extraneural infection route restricts prion conformational variability and attenuates the impact of quaternary structure on infectivity","volume":"20","author":"SC Chang","year":"2024","journal-title":"PLoS Pathog"},{"issue":"32","key":"ppat.1012746.ref028","doi-asserted-by":"crossref","first-page":"e2402726121","DOI":"10.1073\/pnas.2402726121","article-title":"Propagation of distinct CWD prion strains during peripheral and intracerebral challenges of gene-targeted mice","volume":"121","author":"JP DeFranco","year":"2024","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"ppat.1012746.ref029","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.3233\/JPD-240228","article-title":"Are Preformed Fibrils a Model of Parkinson\u2019s Disease?","volume":"14","author":"AL Woerman","year":"2024","journal-title":"J Parkinsons Dis"},{"key":"ppat.1012746.ref030","article-title":"Structurally targeted mutagenesis identifies key residues supporting \u03b1-synuclein misfolding in multiple system atrophy","author":"PM Reis","year":"2024","journal-title":"J Parkinsons Dis"},{"issue":"1","key":"ppat.1012746.ref031","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s40478-019-0804-5","article-title":"Silver staining (Campbell-Switzer) of neuronal alpha-synuclein assemblies induced by multiple system atrophy and Parkinson\u2019s disease brain extracts in transgenic mice","volume":"7","author":"I Lavenir","year":"2019","journal-title":"Acta Neuropathol Commun"},{"key":"ppat.1012746.ref032","doi-asserted-by":"crossref","first-page":"e1002419","DOI":"10.1371\/journal.ppat.1002419","article-title":"Genesis of mammalian prions: from non-infectious amyloid fibrils to a transmissible prion disease","volume":"7","author":"N Makarava","year":"2011","journal-title":"PLoS Pathog"},{"issue":"Pt 1","key":"ppat.1012746.ref033","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1099\/vir.0.19394-0","article-title":"Delay in onset of prion disease for the HY strain of transmissible mink encephalopathy as a result of prior peripheral inoculation with the replication-deficient DY strain","volume":"85","author":"JC Bartz","year":"2004","journal-title":"J Gen Virol"},{"issue":"2","key":"ppat.1012746.ref034","doi-asserted-by":"crossref","first-page":"e1008222","DOI":"10.1371\/journal.ppat.1008222","article-title":"Kinetics of \u03b1-synuclein prions preceding neuropathological inclusions in multiple system atrophy","volume":"16","author":"AL Woerman","year":"2020","journal-title":"PLOS Pathogens"},{"issue":"1","key":"ppat.1012746.ref035","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1128\/JVI.01745-08","article-title":"Prion strain targeting independent of strain-specific neuronal tropism","volume":"83","author":"JI Ayers","year":"2009","journal-title":"J Virol"},{"key":"ppat.1012746.ref036","article-title":"A Novel SNCA A30G Mutation Causes Familial Parkinson\u2019s Disease","author":"H Liu","year":"2021","journal-title":"Mov Disord"},{"issue":"2","key":"ppat.1012746.ref037","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1002\/ana.10795","article-title":"The new mutation, E46K, of \u03b1-synuclein causes Parkinson and Lewy body dementia","volume":"55","author":"JJ Zarranz","year":"2004","journal-title":"Ann Neurol"},{"key":"ppat.1012746.ref038","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1007\/s00401-013-1096-7","article-title":"\u03b1-Synucleinopathy associated with G51D SNCA mutation: a link between Parkinson\u2019s disease and multiple system atrophy?","volume":"125","author":"AP Kiely","year":"2013","journal-title":"Acta Neuropathol"},{"key":"ppat.1012746.ref039","doi-asserted-by":"crossref","first-page":"2180.e1","DOI":"10.1016\/j.neurobiolaging.2014.03.024","article-title":"A novel \u03b1-synuclein mutation A53E associated with atypical multiple system atrophy and Parkinson\u2019s disease-type pathology","volume":"35","author":"P Pasanen","year":"2014","journal-title":"Neurobiol Aging"},{"key":"ppat.1012746.ref040","doi-asserted-by":"crossref","first-page":"248.e7","DOI":"10.1016\/j.neurobiolaging.2017.05.022","article-title":"Homozygous alpha-synuclein p.A53V in familial Parkinson\u2019s disease","volume":"57","author":"H Yoshino","year":"2017","journal-title":"Neurobiol Aging"},{"issue":"5321","key":"ppat.1012746.ref041","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1126\/science.276.5321.2045","article-title":"Mutation in the \u03b1-synuclein gene identified in families with Parkinson\u2019s disease","volume":"276","author":"MH Polymeropoulos","year":"1997","journal-title":"Science"},{"issue":"3","key":"ppat.1012746.ref042","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s00401-022-02403-w","article-title":"Consequences of variability in alpha-synuclein fibril structure on strain biology","volume":"143","author":"SAM Holec","year":"2022","journal-title":"Acta Neuropathol"},{"issue":"18","key":"ppat.1012746.ref043","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00586-18","article-title":"Localized Induction of Wild-Type and Mutant Alpha-Synuclein Aggregation Reveals Propagation along Neuroanatomical Tracts","volume":"92","author":"JI Ayers","year":"2018","journal-title":"J Virol"},{"key":"ppat.1012746.ref044","doi-asserted-by":"crossref","first-page":"5759","DOI":"10.1128\/JVI.76.11.5759-5768.2002","article-title":"Retrograde transport of transmissible mink encephalopathy within descending motor tracts","volume":"76","author":"JC Bartz","year":"2002","journal-title":"J Virol"},{"issue":"4","key":"ppat.1012746.ref045","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0896-6273(02)00682-7","article-title":"Neuronal \u03b1-synucleinopathy with severe movement disorder in mice expressing A53T human \u03b1-synuclein","volume":"34","author":"BI Giasson","year":"2002","journal-title":"Neuron"},{"issue":"3","key":"ppat.1012746.ref046","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s00401-019-01959-4","article-title":"Multiple system atrophy prions retain strain specificity after serial propagation in two different Tg(SNCA*A53T) mouse lines","volume":"137","author":"AL Woerman","year":"2019","journal-title":"Acta Neuropathol"},{"issue":"1","key":"ppat.1012746.ref047","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/s41598-022-26300-0","article-title":"Prion protein amino acid sequence influences formation of authentic synthetic PrP(Sc)","volume":"13","author":"AJ Block","year":"2023","journal-title":"Sci Rep"},{"issue":"3","key":"ppat.1012746.ref048","doi-asserted-by":"crossref","first-page":"252","DOI":"10.4161\/pri.19930","article-title":"Genesis of tramsmissible protein states via deformed templating","volume":"6","author":"N Makarava","year":"2012","journal-title":"Prion"},{"issue":"5","key":"ppat.1012746.ref049","doi-asserted-by":"crossref","first-page":"e1008581","DOI":"10.1371\/journal.ppat.1008581","article-title":"Emergence of prions selectively resistant to combination drug therapy","volume":"16","author":"CM Burke","year":"2020","journal-title":"PLoS Pathog"},{"key":"ppat.1012746.ref050","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1126\/science.1138718","article-title":"A general model of prion strains and their pathogenicity","volume":"318","author":"J Collinge","year":"2007","journal-title":"Science"},{"issue":"5967","key":"ppat.1012746.ref051","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1126\/science.1183218","article-title":"Darwinian evolution of prions in cell culture","volume":"327","author":"J Li","year":"2010","journal-title":"Science"},{"key":"ppat.1012746.ref052","doi-asserted-by":"crossref","first-page":"e1002582","DOI":"10.1371\/journal.ppat.1002582","article-title":"Mutation and selection of prions","volume":"8","author":"C Weissmann","year":"2012","journal-title":"PLoS Pathog"},{"issue":"7","key":"ppat.1012746.ref053","doi-asserted-by":"crossref","first-page":"3592","DOI":"10.1073\/pnas.1917914117","article-title":"The \u03b1-synuclein hereditary mutation E46K unlocks a more stable, pathogenic fibril structure","volume":"117","author":"DR Boyer","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"ppat.1012746.ref054","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1038\/s41594-019-0322-y","article-title":"Structures of fibrils formed by alpha-synuclein hereditary disease mutant H50Q reveal new polymorphs","volume":"26","author":"DR Boyer","year":"2019","journal-title":"Nat Struct Mol Biol"},{"issue":"1","key":"ppat.1012746.ref055","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1038\/s41467-018-05971-2","article-title":"Cryo-EM of full-length \u03b1-synuclein reveals fibril polymorphs with a common structural kernel","volume":"9","author":"B Li","year":"2018","journal-title":"Nat Commun"},{"key":"ppat.1012746.ref056","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.48907","article-title":"Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy","volume":"8","author":"R Guerrero-Ferreira","year":"2019","journal-title":"Elife"},{"key":"ppat.1012746.ref057","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.36402","article-title":"Cryo-EM structure of alpha-synuclein fibrils","volume":"7","author":"R Guerrero-Ferreira","year":"2018","journal-title":"eLife"},{"issue":"9","key":"ppat.1012746.ref058","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1038\/s41422-018-0075-x","article-title":"Amyloid fibril structure of \u03b1-synuclein determined by cryo-electron microscopy","volume":"28","author":"Y Li","year":"2018","journal-title":"Cell Res"},{"issue":"20","key":"ppat.1012746.ref059","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2012435118","article-title":"Wild-type alpha-synuclein inherits the structure and exacerbated neuropathology of E46K mutant fibril strain by cross-seeding","volume":"118","author":"H Long","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"19","key":"ppat.1012746.ref060","doi-asserted-by":"crossref","first-page":"3913","DOI":"10.1016\/j.jmb.2019.07.001","article-title":"Structural Insights into alpha-Synuclein Fibril Polymorphism: Effects of Parkinson\u2019s Disease-Related C-Terminal Truncations","volume":"431","author":"X Ni","year":"2019","journal-title":"J Mol Biol"},{"issue":"4","key":"ppat.1012746.ref061","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/s41422-020-0299-4","article-title":"Cryo-EM structure of full-length alpha-synuclein amyloid fibril with Parkinson\u2019s disease familial A53T mutation","volume":"30","author":"Y Sun","year":"2020","journal-title":"Cell Res"},{"issue":"4","key":"ppat.1012746.ref062","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/2211-5463.13110","article-title":"Seeded assembly in vitro does not replicate the structures of alpha-synuclein filaments from multiple system atrophy","volume":"11","author":"S L\u00f6vestam","year":"2021","journal-title":"FEBS Open Bio"},{"key":"ppat.1012746.ref063","article-title":"Brain-derived and in vitro-seeded alpha-synuclein fibrils exhibit distinct biophysical profiles","author":"SS Lee","year":"2023","journal-title":"bioRxiv"},{"key":"ppat.1012746.ref064","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/0092-8674(86)90875-5","article-title":"Linkage of prion protein and scrapie incubation time genes","volume":"46","author":"GA Carlson","year":"1986","journal-title":"Cell"},{"issue":"10","key":"ppat.1012746.ref065","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1038\/2654","article-title":"Eight prion strains have PrPSc molecules with different conformations","volume":"4","author":"J Safar","year":"1998","journal-title":"Nat Med"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T13:22:10Z","timestamp":1733491330000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1012746"}},"subtitle":[],"editor":[{"given":"Suzette A.","family":"Priola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,6]]},"references-count":65,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1012746","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,6]]}}}