{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:08:34Z","timestamp":1792487314426,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012812","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000}}],"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI087946"],"award-info":[{"award-number":["R01AI087946"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI132818"],"award-info":[{"award-number":["R01AI132818"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Spirochetes are a widespread group of bacteria with a distinct morphology. Some spirochetes are important human pathogens that utilize periplasmic flagella to achieve motility and host infection. The motors that drive the rotation of periplasmic flagella have a unique spirochete-specific feature, termed the collar, crucial for the flat-wave morphology and motility of the Lyme disease spirochete <jats:italic>Borrelia burgdorferi<\/jats:italic>. Here, we deploy cryo-electron tomography and subtomogram averaging to determine high-resolution <jats:italic>in-situ<\/jats:italic> structures of the <jats:italic>B<\/jats:italic>. <jats:italic>burgdorferi<\/jats:italic> flagellar motor. Comparative analysis and molecular modeling of <jats:italic>in-situ<\/jats:italic> flagellar motor structures from <jats:italic>B<\/jats:italic>. <jats:italic>burgdorferi<\/jats:italic> mutants lacking each of the known collar proteins (FlcA, FlcB, FlcC, FlbB, and Bb0236\/FlcD) uncover a complex protein network at the base of the collar. Importantly, our data suggest that FlbB forms a novel periplasmic ring around the rotor but also acts as a scaffold supporting collar assembly and subsequent recruitment of stator complexes. The complex protein network based on the FlbB ring effectively bridges the rotor and 16 torque-generating stator complexes in each flagellar motor, thus contributing to the specialized motility and lifestyle of spirochetes in complex environments.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012812","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T19:36:24Z","timestamp":1736364984000},"page":"e1012812","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["FlbB forms a distinctive ring essential for periplasmic flagellar assembly and motility in Borrelia burgdorferi"],"prefix":"10.1371","volume":"21","author":[{"given":"Jack M.","family":"Botting","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Khalesur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangbiao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua T.","family":"Del Mundo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Hammel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2761-5104","authenticated-orcid":true,"given":"Md 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