{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:45:09Z","timestamp":1792806309289,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1012857","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000}}],"reference-count":92,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["225677\/Z\/22\/Z"],"award-info":[{"award-number":["225677\/Z\/22\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["218518\/Z\/19\/Z"],"award-info":[{"award-number":["218518\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["213455\/Z\/18\/Z"],"award-info":[{"award-number":["213455\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Iron is required to support almost all life; however, levels must be carefully regulated to maintain homeostasis. Although the obligate parasite\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    requires iron, how it responds upon iron limitation has not been investigated. Here, we show that iron depletion triggers significant transcriptional changes in the parasite, including in iron-dependent pathways. We find that a subset of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    transcripts contain stem-loop structures, which have been associated with post-transcriptional iron-mediated regulation in other cellular systems. We validate one of these (found in the 3\u2019 UTR of TGME49_261720) using a reporter cell line. We show that the presence of the stem-loop-containing UTR is sufficient to confer accumulation at the transcript and protein levels under low iron. This response is dose and time-dependent and is specific for iron. The accumulation of transcript is likely driven by an increased reporter mRNA stability under low iron. Interestingly, we find iron-mediated changes in mRNA stability in around 400 genes. To examine the potential mechanism of this stability, we tested aconitase interaction with mRNA in low iron and found 43 enriched transcripts, but no specific interaction with our reporter UTR. However, the endogenous UTR led to maintenance of protein levels and increased survival of the parasite under low iron. Our data demonstrate the existence of iron-mediated post-transcriptional regulation in\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    for the first time; and suggests iron-mediated regulation may be important to the parasite in low iron environments.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1012857","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T13:49:19Z","timestamp":1738590559000},"page":"e1012857","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Iron-mediated post-transcriptional regulation in Toxoplasma gondii"],"prefix":"10.1371","volume":"21","author":[{"given":"Megan A.","family":"Sloan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Aghabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Mrvova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8428-0374","authenticated-orcid":true,"given":"Clare R.","family":"Harding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,2,3]]},"reference":[{"key":"ppat.1012857.ref001","doi-asserted-by":"crossref","first-page":"e1008499","DOI":"10.1371\/journal.ppat.1008499","article-title":"Toxoplasma gondii requires its plant-like heme biosynthesis pathway for 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