{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T21:27:01Z","timestamp":1772227621811,"version":"3.50.1"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30-CA044579"],"award-info":[{"award-number":["P30-CA044579"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018088","name":"School of Medicine, University of Virginia","doi-asserted-by":"publisher","award":["Myles H. Thaler Endowed Professorship"],"award-info":[{"award-number":["Myles H. 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In this study, we investigated the impact of variation in Rev-RRE functional activity on HIV-1 replication kinetics and reactivation from latency. Using a novel HIV-1 viral vector with an interchangeable Rev cassette, we engineered viruses with two diverse Rev functional activities and demonstrated that higher Rev-RRE activity confers greater viral replication capacity while maintaining a constant level of Nef expression. In addition, a low Rev activity virus rapidly acquired a compensatory mutation in the RRE that significantly increased Rev-RRE activity and replication. In a latency model, proviruses with differing Rev-RRE activity levels varied in the efficiency of viral reactivation, affecting both initial viral release and subsequent replication kinetics. These results demonstrate that activity differences in the Rev-RRE axis among different viral isolates have important implications for HIV replication dynamics and persistence. Importantly, our findings indicate that bolstering Rev\/RRE activity could be explored as part of latency reversal strategies in HIV cure efforts.<\/jats:p>","DOI":"10.1371\/journal.ppat.1012885","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T13:23:19Z","timestamp":1747315399000},"page":"e1012885","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies"],"prefix":"10.1371","volume":"21","author":[{"given":"Godfrey A.","family":"Dzhivhuho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick E. 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