{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:17:16Z","timestamp":1777940236020,"version":"3.51.4"},"reference-count":107,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A_175557"],"award-info":[{"award-number":["31003A_175557"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_200706"],"award-info":[{"award-number":["310030_200706"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_169386"],"award-info":[{"award-number":["310030_169386"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_188813"],"award-info":[{"award-number":["310030_188813"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["CRSII5_189921"],"award-info":[{"award-number":["CRSII5_189921"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RTG2581"],"award-info":[{"award-number":["RTG2581"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>The causative agent of Legionnaires\u2019 disease, <jats:italic>Legionella pneumophila<\/jats:italic>, is an amoebae-resistant environmental bacterium, which replicates intracellularly in a distinct compartment, the \u201c<jats:italic>Legionella<\/jats:italic>-containing vacuole\u201d (LCV). <jats:italic>L. pneumophila<\/jats:italic> employs the \u03b1-hydroxyketone compound LAI-1 (<jats:italic>Legionella<\/jats:italic> autoinducer-1) for intra-species and inter-kingdom signaling. LAI-1 promotes intracellular replication and inhibits the migration of mammalian cells and <jats:italic>Dictyostelium discoideum<\/jats:italic>. In this study, we revealed that LAI-1 and \u201cclickable\u201d azido-LAI-1 derivatives inhibit the migration of <jats:italic>D. discoideum<\/jats:italic> and localize to LCVs. Azido-LAI-1 colocalizes with the LCV markers calnexin, P4C, and AmtA, but not with mitochondrial or lipid droplet markers. Intriguingly, LAI-1-dependent inhibition of <jats:italic>D. discoideum<\/jats:italic> migration involves the single guanylate-binding protein (GBP), a member of the GBP family of large GTPases, which in metazoan organisms promote cell autonomous immunity. <jats:italic>D. discoideum<\/jats:italic> lacking GBP (\u0394<jats:italic>gnbp<\/jats:italic>) allows more efficient intracellular replication of <jats:italic>L. pneumophila<\/jats:italic>, without apparently compromising LCV formation or integrity, and GBP-GFP localizes to the ER at LCV-ER membrane contact sites (MCS). However, the peri-LCV localization of LAI-1 and GBP is not mutually dependent. Synthetic LAI-1 inhibits the expansion\/remodeling of LCVs (but not vacuoles harboring avirulent <jats:italic>L. pneumophila<\/jats:italic>) in a GBP-dependent manner. Taken together, the work shows that LAI-1 localizes to LCVs, and LAI-1-dependent inter-kingdom signaling involves <jats:italic>D. discoideum<\/jats:italic> GBP, which localizes to LCV-ER MCS and acts as an antimicrobial factor by restricting the intracellular growth of <jats:italic>L. pneumophila<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.ppat.1013026","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T18:17:25Z","timestamp":1745950645000},"page":"e1013026","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inter-kingdom signaling by the Legionella autoinducer LAI-1 involves the antimicrobial guanylate binding protein GBP"],"prefix":"10.1371","volume":"21","author":[{"given":"Franziska","family":"Solger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Rauch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Vormittag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingzhen","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyudmil","family":"Raykov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Charki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Katic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Letourneur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Soldati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Seibel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5462-9301","authenticated-orcid":true,"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"ppat.1013026.ref001","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev-genet-102108-134304","article-title":"Bacterial quorum-sensing network architectures","volume":"43","author":"W-L Ng","year":"2009","journal-title":"Annu Rev Genet"},{"issue":"2","key":"ppat.1013026.ref002","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.mib.2009.01.003","article-title":"New developments in microbial interspecies signaling","volume":"12","author":"EA Shank","year":"2009","journal-title":"Curr Opin Microbiol"},{"issue":"2","key":"ppat.1013026.ref003","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.mib.2009.01.006","article-title":"Inter-kingdom signaling: chemical language between bacteria and host","volume":"12","author":"AR Pacheco","year":"2009","journal-title":"Curr Opin Microbiol"},{"issue":"2","key":"ppat.1013026.ref004","doi-asserted-by":"crossref","first-page":"e01748","DOI":"10.1128\/mBio.01748-15","article-title":"What a dinner party! 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