{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:34Z","timestamp":1787022214885,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013027","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000}}],"reference-count":74,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008349","name":"Boehringer Ingelheim","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001021","name":"Damon Runyon Cancer Research Foundation","doi-asserted-by":"publisher","award":["DRG-2415-20"],"award-info":[{"award-number":["DRG-2415-20"]}],"id":[{"id":"10.13039\/100001021","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI179642"],"award-info":[{"award-number":["R01AI179642"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Mycobacterium abscessus<\/jats:italic>\n                    is a pulmonary pathogen that exhibits intrinsic resistance to antibiotics, but the factors driving this resistance are incompletely understood. Insufficient intracellular drug accumulation could explain broad-spectrum resistance, but whether antibiotics fail to accumulate in\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    and the mechanisms required for drug exclusion remain poorly understood. We measured antibiotic accumulation in\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    using mass spectrometry and found a wide range of drug accumulation across clinically relevant antibiotics. Of these compounds, linezolid accumulates the least, suggesting that inadequate uptake impacts its efficacy. We utilized transposon mutagenesis screening to identify genes that cause linezolid resistance and found multiple transporters that promote membrane permeability or efflux, including an uncharacterized protein that effluxes linezolid and several chemically related antibiotics. This demonstrates that membrane permeability and drug efflux are critical mechanisms of antibiotic resistance in\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    and suggests that targeting membrane transporters could potentiate the efficacy of certain antibiotics.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013027","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T13:30:37Z","timestamp":1744291837000},"page":"e1013027","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Efflux pumps and membrane permeability contribute to intrinsic antibiotic resistance in Mycobacterium abscessus"],"prefix":"10.1371","volume":"21","author":[{"given":"Kerry","family":"McGowen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Funck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Zinga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian D.","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chidiebere","family":"Akusobi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia M.","family":"Denkinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Rubin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5765-0459","authenticated-orcid":true,"given":"Mark R.","family":"Sullivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"issue":"4","key":"ppat.1013027.ref001","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1164\/rccm.200604-571ST","article-title":"An official ATS\/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases","volume":"175","author":"DE Griffith","year":"2007","journal-title":"Am J Respir Crit Care Med"},{"key":"ppat.1013027.ref002","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.3389\/fmicb.2019.01977","article-title":"Clinical efficacy and adverse effects of antibiotics used to treat Mycobacterium abscessus pulmonary disease","volume":"10","author":"J Chen","year":"2019","journal-title":"Front Microbiol"},{"issue":"1","key":"ppat.1013027.ref003","doi-asserted-by":"crossref","first-page":"1900140","DOI":"10.1183\/13993003.01991-2018","article-title":"Mycobacterium abscessus pulmonary disease: individual patient data meta-analysis","volume":"54","author":"N Kwak","year":"2019","journal-title":"Eur Respir J"},{"issue":"4","key":"ppat.1013027.ref004","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1093\/jac\/dkr578","article-title":"Mycobacterium abscessus: a new antibiotic nightmare","volume":"67","author":"R Nessar","year":"2012","journal-title":"J Antimicrob Chemother"},{"issue":"4","key":"ppat.1013027.ref005","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1128\/AAC.01275-08","article-title":"A novel gene, erm(41), confers inducible macrolide resistance to clinical isolates of Mycobacterium abscessus but is absent from Mycobacterium chelonae","volume":"53","author":"KA Nash","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"ppat.1013027.ref006","doi-asserted-by":"crossref","first-page":"e00119-18","DOI":"10.1128\/AAC.00119-18","article-title":"High levels of intrinsic tetracycline resistance in mycobacterium abscessus are conferred by a tetracycline-modifying monooxygenase","volume":"62","author":"P Rudra","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"ppat.1013027.ref007","doi-asserted-by":"crossref","first-page":"e00978-21","DOI":"10.1128\/AAC.00978-21","article-title":"Blocking bacterial naphthohydroquinone oxidation and ADP-ribosylation improves activity of rifamycins against Mycobacterium abscessus","volume":"65","author":"U Ganapathy","year":"2021","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"ppat.1013027.ref008","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41579-019-0273-7","article-title":"The mycobacterial cell envelope - 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