{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T12:00:07Z","timestamp":1781697607185,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013061","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000}}],"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI167663"],"award-info":[{"award-number":["R01AI167663"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI186385"],"award-info":[{"award-number":["R21AI186385"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA290700"],"award-info":[{"award-number":["R01CA290700"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R21CA299587"],"award-info":[{"award-number":["R21CA299587"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["MBGI-24-1255200-01-MBG"],"award-info":[{"award-number":["MBGI-24-1255200-01-MBG"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Kaposi\u2019s sarcoma-associated herpesvirus (KSHV) genome contains a terminal repeats (TR) sequence. Previous studies demonstrated that KSHV TR functions as a gene enhancer for inducible lytic gene promoters. Gene enhancers anchor bromodomain-containing protein 4 (BRD4) at specific genomic region, where BRD4 interacts flexibly with transcription-related proteins through its intrinsically disordered domain and exerts transcription regulatory function. Here, we generated recombinant KSHV with reduced TR copy numbers and studied BRD4 recruitment and its contributions to the inducible promoter activation. Reducing the TR copy numbers from 21 (TR21) to 5 (TR5) strongly attenuated viral gene expression during\n                    <jats:italic>de novo<\/jats:italic>\n                    infection and impaired reactivation. The EF1\u03b1 promoter encoded in the KSHV BAC backbone also showed reduced promoter activity, suggesting a global attenuation of transcription activity within TR5 latent mini-chromatin. Isolation of reactivating cells confirmed that the reduced inducible gene transcription from TR-shortened DNA template is mediated by decreased efficacy of BRD4 recruitment to viral gene promoters. Separating the reactivating iSLK cell population from non-responders showed that reactivatable iSLK cells harbored larger LANA nuclear bodies (NBs) compared to non-responders. The cells with larger LANA NBs, either due to prior transcription activation or TR copy number, supported KSHV reactivation more efficiently than those with smaller LANA NBs. With auxin-inducible LANA degradation, we confirmed that LANA is responsible for BRD4 occupancies on latent chromatin. Finally, with purified fluorescence-tagged proteins, we demonstrated that BRD4 is required for LANA to form liquid-liquid phase-separated dots. The inclusion of TR DNA fragments further facilitated the formation of larger BRD4-containing LLPS with LANA as similar to the \u201ccellular enhancer dot\u201d formed by transcription factor-DNA bindings. These results suggest that LANA TR binding establishes an enhancer domain for infected KSHV episomes. The strength of this enhancer, regulated by TR length or transcription memories from prior activation, determines the degree of KSHV lytic replication.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013061","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T19:00:49Z","timestamp":1764874849000},"page":"e1013061","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["KSHV TR deletion episomes uncover enhancer\u2013promoter dynamics in gene regulation"],"prefix":"10.1371","volume":"21","author":[{"given":"Tomoki","family":"Inagaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashish","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang-Hsin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somayeh","family":"Komaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonna M.","family":"Espera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S. A.","family":"Bautista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Izumiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9184-2603","authenticated-orcid":true,"given":"Yoshihiro","family":"Izumiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"issue":"1","key":"ppat.1013061.ref001","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1006\/viro.1997.8713","article-title":"The structure and coding organization of the genomic termini of Kaposi\u2019s sarcoma-associated herpesvirus","volume":"236","author":"M 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Uppal","year":"2014","journal-title":"Viruses"},{"issue":"2","key":"ppat.1013061.ref005","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01386-23","article-title":"Kaposi\u2019s sarcoma-associated herpesvirus terminal repeat regulates inducible lytic gene promoters","volume":"98","author":"Y Izumiya","year":"2024","journal-title":"J Virol"},{"issue":"6","key":"ppat.1013061.ref006","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00179-24","article-title":"KSHV genome harbors both constitutive and lytically induced enhancers","volume":"98","author":"N Roy Chowdhury","year":"2024","journal-title":"J Virol"},{"issue":"1","key":"ppat.1013061.ref007","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s12929-020-00637-y","article-title":"PAN RNA: transcriptional exhaust from a viral engine","volume":"27","author":"M Campbell","year":"2020","journal-title":"J Biomed 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