{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T09:27:38Z","timestamp":1773221258510,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013075","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000}}],"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100016683","name":"Iowa Agriculture and Home Economics Experiment Station, Iowa State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016683","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Hatch"],"award-info":[{"award-number":["Hatch"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Iowa State funds"},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2019-67013-29350"],"award-info":[{"award-number":["2019-67013-29350"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Northwest Potato Research Consortium"},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Hatch WNP7003632"],"award-info":[{"award-number":["Hatch WNP7003632"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Hatch W5186"],"award-info":[{"award-number":["Hatch W5186"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The root-knot nematode (RKN)\n                    <jats:italic>\n                      <jats:italic>Meloidogyne chitwoodi<\/jats:italic>\n                    <\/jats:italic>\n                    is a threat for potato production in the western United States (U.S.), negatively impacting potato yield and product value\n                    <jats:italic>\n                      <jats:italic>. Meloidogyne chitwoodi<\/jats:italic>\n                    <\/jats:italic>\n                    produce proteins, called effectors, in their esophageal glands that are secreted during parasitism and play integral roles in plant-nematode interactions. Because the esophageal glands are the main effector secretory organs, we isolated juvenile gland cells and performed gland transcriptome analysis with our newly improved genome annotation. The gland-specific transcriptome data gave us an enrichment of gland-localized genes, which was validated by\n                    <jats:italic>\n                      <jats:italic>in situ<\/jats:italic>\n                    <\/jats:italic>\n                    hybridization. The gland transcriptome analysis led to the identification of 125 effector candidates. One of the effectors that was highly expressed in the pre-parasitic J2 gland tissue, referred to as McGland26, was further characterized.\n                    <jats:italic>\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    <\/jats:italic>\n                    expressing\n                    <jats:italic>\n                      <jats:italic>McGland26<\/jats:italic>\n                    <\/jats:italic>\n                    showed enhanced susceptibility to\n                    <jats:italic>\n                      <jats:italic>M. chitwoodi<\/jats:italic>\n                    <\/jats:italic>\n                    . However, the ectopic expression of\n                    <jats:italic>\n                      <jats:italic>McGland26 in planta<\/jats:italic>\n                    <\/jats:italic>\n                    did not suppress plant defenses, suggesting that this effector might be involved in processes other than interfering with plant immunity. Our data show that by using the gland transcriptome, a good quality genome annotation, and stringent selection criteria, we can increase the efficiency of effector identification, which can be used to develop more sustainable management tools.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013075","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T18:46:27Z","timestamp":1762541187000},"page":"e1013075","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome reannotation and gland-specific transcriptome analysis identify new effector candidates in Meloidogyne chitwoodi"],"prefix":"10.1371","volume":"21","author":[{"given":"Marcella","family":"Teixeira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itsuhiro","family":"Ko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sapinder","family":"Bali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas R.","family":"Maier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J.","family":"Baum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8365-9131","authenticated-orcid":true,"given":"Cynthia","family":"Gleason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,11,7]]},"reference":[{"issue":"8","key":"ppat.1013075.ref001","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1094\/MPMI-12-20-0337-A","article-title":"Nematode Genome Announcement: Draft Genome of Meloidogyne chitwoodi, an Economically Important Pest of Potato in the Pacific Northwest","volume":"34","author":"S Bali","year":"2021","journal-title":"Mol Plant Microbe Interact"},{"issue":"12","key":"ppat.1013075.ref002","doi-asserted-by":"crossref","DOI":"10.1002\/cpz1.323","article-title":"BUSCO: Assessing Genomic Data Quality and Beyond","volume":"1","author":"M Manni","year":"2021","journal-title":"Curr 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