{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:07:39Z","timestamp":1783649259213,"version":"3.55.0"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"crossref","award":["JP21gm0910010"],"award-info":[{"award-number":["JP21gm0910010"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p><jats:italic>Cryptococcus neoformans<\/jats:italic> (<jats:italic>Cn<\/jats:italic>) is a fungal pathogen responsible for cryptococcal meningitis, which accounts for 15% of AIDS-related deaths. Recent studies have shown that the absence of sterol \u03b2-glucosidase (EGCrP2, also known as Sgl1) in <jats:italic>Cn<\/jats:italic> significantly attenuates its virulence in a mouse infection model. However, the mechanisms underlying this virulence attenuation remain unclear. In this study, we observed a significant increase in dead cells after 3 days of culture of <jats:italic>SGL1<\/jats:italic>-deficient <jats:italic>Cn<\/jats:italic> (<jats:italic>sgl1<\/jats:italic>\u0394, KO) at 37\u00b0C, compared with wild-type (WT) and <jats:italic>SGL1<\/jats:italic>-reconstituted <jats:italic>Cn<\/jats:italic> (<jats:italic>sgl1<\/jats:italic>\u0394::<jats:italic>SGL1<\/jats:italic>, RE). qPCR analysis of WT, KO, and RE strains indicated that autophagy-related genes (<jats:italic>ATG<\/jats:italic>s) were significantly downregulated in KO strain. Atg8-dependent GFP translocation to the vacuole was significantly delayed in KO strain under starvation conditions. This autophagy dysfunction was identified as the primary cause of the increased cell death observed in KO strain under nitrogen starvation conditions at 37\u00b0C. EGCrP2\/Sgl1 is predominantly localized in the vacuoles of <jats:italic>Cn<\/jats:italic>, and its deletion results in the accumulation of not only ergosterol \u03b2-glucoside (EG), as previously reported, but also acylated EGs (AEGs). AEGs were much more potent than EG in activating the C-type lectin receptor Mincle in mice, rats, and humans. AEGs were released from KO strain via extracellular vesicles (EVs). Chemically synthesized 18:1-EG and EVs derived from KO strain, but not WT or RE strains, enhanced cytokine production in murine and human dendritic cells. AEG-dependent cytokine production was markedly reduced in dendritic cells from Mincle-deficient mice, and the number of KO strain in lung tissue from Mincle-deficient mice was substantially higher than wild-type mice on day 3 after infection. Intranasal administration of acylated sitosterol \u03b2-glucoside increased Mincle expression and cytokine production and reduced the <jats:italic>Cn<\/jats:italic> burden in lung tissue of <jats:italic>Cn<\/jats:italic>-infected mice. These findings suggest that autophagy dysfunction in KO strain and the\u00a0host\u00a0innate immune response via the AEG-dependent Mincle activation are critical in reducing <jats:italic>Cn<\/jats:italic> virulence in mice.<\/jats:p>","DOI":"10.1371\/journal.ppat.1013089","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T17:41:03Z","timestamp":1745516463000},"page":"e1013089","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Vacuolar sterol \u03b2-glucosidase EGCrP2\/Sgl1 deficiency in Cryptococcus neoformans: Dysfunctional autophagy and Mincle-dependent immune activation as targets of novel antifungal 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Cloning and Functional Expression of UGT Genes Encoding Sterol Glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum*. 1999 [cited 2023 May 31]; Available from: http:\/\/www.jbc.org","DOI":"10.1074\/jbc.274.19.13048"},{"issue":"10","key":"ppat.1013089.ref032","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1093\/glycob\/cwv045","article-title":"Ergosteryl-\u03b2-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae","volume":"25","author":"T Watanabe","year":"2015","journal-title":"Glycobiology"},{"key":"ppat.1013089.ref033","doi-asserted-by":"crossref","first-page":"14712","DOI":"10.1074\/jbc.271.25.14712","article-title":"The multidrug resistance-associated protein (MRP) subfamily (Yrs1\/Yor1) of Saccharomyces cerevisiae is important for the tolerance to a broad range of organic anions","volume":"271","author":"Z Cui","year":"1996","journal-title":"J Biol Chem"},{"key":"ppat.1013089.ref034","first-page":"177","article-title":"Physiological and Pathological Functions of CARD9 Signaling in the Innate Immune System","volume":"429","author":"L Vornholz","year":"2020","journal-title":"Curr Top Microbiol Immunol"},{"key":"ppat.1013089.ref035","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.3389\/fmicb.2019.02690","article-title":"Conserved Autophagy Pathway Contributes to Stress Tolerance and Virulence and Differentially Controls Autophagic Flux Upon Nutrient Starvation in Cryptococcus neoformans","volume":"10","author":"X Zhao","year":"2019","journal-title":"Front Microbiol"},{"issue":"13","key":"ppat.1013089.ref036","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1084\/jem.20091750","article-title":"Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle","volume":"206","author":"E Ishikawa","year":"2009","journal-title":"J Exp Med"},{"issue":"6","key":"ppat.1013089.ref037","article-title":"The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors","volume":"15","author":"A Thompson","year":"2019","journal-title":"PLoS Pathog [Internet]"},{"issue":"12","key":"ppat.1013089.ref038","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1128\/EC.00111-14","article-title":"Interaction of Cryptococcus neoformans extracellular vesicles with the cell wall","volume":"13","author":"JM Wolf","year":"2014","journal-title":"Eukaryot Cell"},{"issue":"1","key":"ppat.1013089.ref039","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-018-03991-6","article-title":"Pathogen-derived extracellular vesicles mediate virulence in the fatal human pathogen Cryptococcus gattii","volume":"9","author":"E Bielska","year":"2018","journal-title":"Nat Commun"},{"key":"ppat.1013089.ref040","author":"B Tancini"},{"issue":"1","key":"ppat.1013089.ref041","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.1038\/s41467-023-40680-5","article-title":"Mitochondria are secreted in extracellular vesicles when lysosomal function is impaired","volume":"14","author":"W Liang","year":"2023","journal-title":"Nat Commun"},{"issue":"6","key":"ppat.1013089.ref042","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1038\/s41385-022-00570-3","article-title":"Vaccine protection by Cryptococcus neoformans \u0394sgl1 is mediated by \u03b3\u03b4 T cells via TLR2 signaling","volume":"15","author":"TG Normile","year":"2022","journal-title":"Mucosal Immunol"},{"issue":"10","key":"ppat.1013089.ref043","article-title":"Role of Dectin-2 in the phagocytosis of Cryptococcus neoformans by dendritic cells. 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