{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:44:18Z","timestamp":1788313458591,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013207","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000}}],"reference-count":94,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI143768"],"award-info":[{"award-number":["R01 AI143768"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI171356"],"award-info":[{"award-number":["R21 AI171356"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002590","name":"American Lung Association","doi-asserted-by":"publisher","award":["CA-1268681"],"award-info":[{"award-number":["CA-1268681"]}],"id":[{"id":"10.13039\/100002590","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Potassium (K\n                    <jats:sup>+<\/jats:sup>\n                    ) is the most abundant intracellular cation, but much remains unknown regarding how K\n                    <jats:sup>+<\/jats:sup>\n                    homeostasis is integrated with other key bacterial biology aspects. Here, we show that K\n                    <jats:sup>+<\/jats:sup>\n                    homeostasis disruption (CeoBC K\n                    <jats:sup>+<\/jats:sup>\n                    uptake system deletion) impedes\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (Mtb) response to, and growth in, cholesterol, a critical carbon source during infection, with K\n                    <jats:sup>+<\/jats:sup>\n                    augmenting activity of the Mtb ATPase MceG that is vital for bacterial cholesterol import. Reciprocally, cholesterol directly binds to CeoB, modulating its function, with a residue critical for this interaction identified. Finally, cholesterol binding-deficient CeoB mutant Mtb are attenuated for growth in lipid-rich foamy macrophages and\n                    <jats:italic>in vivo<\/jats:italic>\n                    colonization. Our findings raise the concept of a role for cholesterol as a key co-factor, beyond its role as a carbon source, and illuminate how changes in intrabacterial K\n                    <jats:sup>+<\/jats:sup>\n                    levels can act as part of the metabolic adaptation critical for bacterial survival and growth in the host.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013207","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T13:37:52Z","timestamp":1747921072000},"page":"e1013207","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Cholesterol metabolism and intrabacterial potassium homeostasis are intrinsically related in Mycobacterium tuberculosis"],"prefix":"10.1371","volume":"21","author":[{"given":"Yue","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berge","family":"Hagopian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1093-2576","authenticated-orcid":true,"given":"Shumin","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"issue":"1","key":"ppat.1013207.ref001","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1085\/jgp.201711835","article-title":"A perspective on Na and K channel inactivation","volume":"150","author":"CM Armstrong","year":"2017","journal-title":"J Gen 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Masin","year":"2017","journal-title":"Sci Rep"},{"key":"ppat.1013207.ref083","doi-asserted-by":"crossref","first-page":"275","DOI":"10.3389\/fcimb.2018.00275","article-title":"The Deconstructed Granuloma: A Complex High-Throughput Drug Screening Platform for the Discovery of Host-Directed Therapeutics Against Tuberculosis","volume":"8","author":"L Huang","year":"2018","journal-title":"Front Cell Infect Microbiol"},{"issue":"12","key":"ppat.1013207.ref084","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0015667","article-title":"Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants in vitro and in vivo","volume":"5","author":"A Blumenthal","year":"2010","journal-title":"PLoS One"},{"issue":"7","key":"ppat.1013207.ref085","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001355","article-title":"Targeting Mycobacterium tuberculosis response to environmental cues for the development of effective antitubercular drugs","volume":"19","author":"RC Lavin","year":"2021","journal-title":"PLoS Biol"},{"issue":"5","key":"ppat.1013207.ref086","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.chom.2007.09.006","article-title":"Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues","volume":"2","author":"KH Rohde","year":"2007","journal-title":"Cell Host Microbe"},{"issue":"4","key":"ppat.1013207.ref087","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"8","key":"ppat.1013207.ref088","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1010331","article-title":"PrrA modulates Mycobacterium tuberculosis response to multiple environmental cues and is critically regulated by serine\/threonine protein kinases","volume":"18","author":"D Giacalone","year":"2022","journal-title":"PLoS Genet"},{"issue":"10","key":"ppat.1013207.ref089","doi-asserted-by":"crossref","first-page":"6788","DOI":"10.1074\/jbc.M112.445056","article-title":"Intracellular Mycobacterium tuberculosis exploits host-derived fatty acids to limit metabolic stress","volume":"288","author":"W Lee","year":"2013","journal-title":"J Biol Chem"},{"issue":"3","key":"ppat.1013207.ref090","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1111\/j.1365-2958.2011.07601.x","article-title":"aprABC: a Mycobacterium tuberculosis complex-specific locus that modulates pH-driven adaptation to the macrophage phagosome","volume":"80","author":"RB Abramovitch","year":"2011","journal-title":"Mol Microbiol"},{"issue":"3","key":"ppat.1013207.ref091","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010459","article-title":"Spatial relationships of intra-lesion heterogeneity in Mycobacterium tuberculosis microenvironment, 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