{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:33:21Z","timestamp":1787740401046,"version":"build-2784847793"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013268","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000}}],"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005270","name":"Fujian Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["No. 2024J011223"],"award-info":[{"award-number":["No. 2024J011223"]}],"id":[{"id":"10.13039\/501100005270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005270","name":"Fujian Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["No. 2022Y4003"],"award-info":[{"award-number":["No. 2022Y4003"]}],"id":[{"id":"10.13039\/501100005270","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fuzhou Municipal Health Commission","award":["No. 2022-S-rc5"],"award-info":[{"award-number":["No. 2022-S-rc5"]}]},{"name":"National Key Research & Development Plan","award":["No. 2023YFC3304304"],"award-info":[{"award-number":["No. 2023YFC3304304"]}]},{"DOI":"10.13039\/501100008859","name":"Fuzhou University","doi-asserted-by":"publisher","award":["No. 2024T021"],"award-info":[{"award-number":["No. 2024T021"]}],"id":[{"id":"10.13039\/501100008859","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fujian Provincial Major Health Research Project","award":["No. 2022ZD01001"],"award-info":[{"award-number":["No. 2022ZD01001"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic> is a notable opportunistic pathogen responsible for severe hospital-acquired infections, with multidrug-resistant strains posing significant treatment challenges. Phage therapy, which employs bacteriophages as natural bacterial antagonists, has gained renewed attention as a promising solution to combat antibiotic-resistant infections. In this study, we isolated and characterized a novel virulent phage, vB_AbaS_qsb1, which specifically lyses <jats:italic>A.baumannii<\/jats:italic>. Phylogenetic and genomic analyses indicate that vB_AbaS_qsb1 is the founding member of a previously unreported genus, which we propose to name <jats:italic>Acinibactriovirus<\/jats:italic>, with <jats:italic>Acinibactriovirus lysinus<\/jats:italic> as the type species. vB_AbaS_qsb1 demonstrated robust stability across diverse temperature and pH ranges, a short latent period, and no known virulence or antibiotic resistance genes within its 54,713\u2009bp dsDNA genome. Safety assessments showed that high-dose vB_AbaS_qsb1 induced no adverse effects in mice, with histopathology confirming its safety profile. Therapeutic experiments further indicated that vB_AbaS_qsb1 provided at least 50% protection against <jats:italic>A.baumannii<\/jats:italic>-induced pneumonia, significantly reducing bacterial loads and inflammation markers, while maintaining high phage titers in lung tissue.This study introduces vB_AbaS_qsb1 as a promising candidate for phage therapy against <jats:italic>A.baumannii<\/jats:italic>, offering both innovative insights and a valuable framework for future isolation, genomic characterization, and efficacy evaluation of phages targeting antibiotic-resistant bacteria.<\/jats:p>","DOI":"10.1371\/journal.ppat.1013268","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T17:39:56Z","timestamp":1750441196000},"page":"e1013268","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["A novel genus of virulent phage targeting Acinetobacter baumannii: Efficacy and safety 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