{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:20:14Z","timestamp":1791382814813,"version":"4.3.3"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013383","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000}}],"reference-count":122,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI171573"],"award-info":[{"award-number":["R21 AI171573"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R32 GM149202"],"award-info":[{"award-number":["R32 GM149202"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Georgia Department of Infectious Diseases"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Human pathogenic filarial nematodes of the family Onchocercidae, including\n                    <jats:italic>Brugia malayi<\/jats:italic>\n                    and\n                    <jats:italic>Onchocerca volvulus,<\/jats:italic>\n                    cause debilitating filarial diseases such as lymphatic filariasis and river blindness. These arthropod-borne pathogens are obligately colonized by the Gram-negative intracellular alphaproteobacterium,\n                    <jats:italic>Wolbachia,<\/jats:italic>\n                    which is essential for nematode sexual reproduction, long-term survival, and pathogenicity in the mammalian host. Like many intracellular bacteria,\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    likely uses numerous surface-exposed and secreted effector proteins to regulate its ability to persist and replicate within nematode host cells. However, due to the inability to cultivate\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    in the laboratory and the genetic intractability of both filarial nematodes and the bacterium, the molecular underpinnings that define the bacterium:nematode relationship are almost completely unknown. In this work, we show that the expression of a\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    outer membrane lipoprotein,\n                    <jats:italic>w<\/jats:italic>\n                    Bm0152, in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    inhibits the activity of the Endosomal Sorting Complex Required for Transport (ESCRT), a highly conserved complex essential for autophagy, endosomal maturation, nuclear envelope repair and viral budding in eukaryotic cells. Wbm0152 expression strongly disrupts endosomal maturation, leading to defects in ubiquitylated protein turnover. Using in vivo bimolecular fluorescence complementation, we find that Wbm0152 interacts with the Vps2p subunit of the ESCRT-III subcomplex as well as the Vps2p ortholog (BmVps2, Bm6583b) from a\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    host nematode,\n                    <jats:italic>Brugia malayi<\/jats:italic>\n                    . These data suggest a novel role of ESCRT in\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    persistence, providing insight into the elusive relationship between these two organisms.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013383","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T18:44:12Z","timestamp":1765478652000},"page":"e1013383","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Wbm0152, an outer membrane lipoprotein of the Wolbachia endosymbiont of Brugia malayi, inhibits yeast ESCRT complex activity"],"prefix":"10.1371","volume":"21","author":[{"given":"Lindsay","family":"Berardi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alora","family":"Colvin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"West","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Odorizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2415-412X","authenticated-orcid":true,"given":"Vincent J.","family":"Starai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"issue":"8291","key":"ppat.1013383.ref001","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0140-6736(82)91026-1","article-title":"Efficacy and tolerance of ivermectin in human onchocerciasis","volume":"2","author":"MA Aziz","year":"1982","journal-title":"Lancet"},{"issue":"4","key":"ppat.1013383.ref002","doi-asserted-by":"crossref","first-page":"591","DOI":"10.4269\/ajtmh.1993.48.591","article-title":"Treatment of subperiodic Brugia malayi infection with a single dose of ivermectin","volume":"48","author":"JW Mak","year":"1993","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"ppat.1013383.ref003","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1080\/00034983.2000.11813583","article-title":"Two-year follow-up of the microfilaraemia of asymptomatic brugian 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