{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:34Z","timestamp":1787022214621,"version":"build-2736575974"},"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210103610"],"award-info":[{"award-number":["DP210103610"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210103610"],"award-info":[{"award-number":["DP210103610"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    is a Gram-negative bacterium that causes a range of distinct diseases in livestock animals. Different\n                    <jats:italic>P. multocida<\/jats:italic>\n                    diseases are associated with different capsule and lipopolysaccharide (LPS) types, but little else is known about what underpins this disease specificity. In this study, we utilised transposon-directed insertion site sequencing (TraDIS) to identify genes required for growth in rich media, and genes important for survival during systemic infections in BALB\/c mice, for two diverse\n                    <jats:italic>P. multocida<\/jats:italic>\n                    strains; VP161 (capsule type A and LPS type L1) and M1404 (capsule type B and LPS type L2). Analysis of growth in heart infusion broth showed that both VP161 and M1404 shared 461 genes essential for growth in rich media, with 95% of these rich media-essential genes present in all publicly available closed\n                    <jats:italic>P. multocida<\/jats:italic>\n                    genomes.\n                    <jats:italic>In vivo<\/jats:italic>\n                    fitness analysis identified 63 and 94 genes important for VP161 and M1404 survival in BALB\/c mice, respectively. Only 35 homologs were identified as important for survival in both strains, showing that conserved biological systems can be differentially important for different\n                    <jats:italic>P. multocida<\/jats:italic>\n                    strains. Investigation of proteins involved in the catabolite response showed that an active cyclic-adenosine monophosphate (cAMP) receptor protein (CRP) was required for maximal fitness in M1404. Furthermore, disrupting CRP or cAMP production also reduced capsule production in M1404, but increased capsule production in VP161, demonstrating that these\n                    <jats:italic>P. multocida<\/jats:italic>\n                    strains have different regulatory systems for crucial virulence factors.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013398","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T18:30:31Z","timestamp":1766428231000},"page":"e1013398","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative analysis of shared and unique mechanisms important for diverse strains of Pasteurella multocida to cause systemic infection in mice"],"prefix":"10.1371","volume":"21","author":[{"given":"Thomas R.","family":"Smallman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xenia","family":"Kostoulias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faye C.","family":"Morris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Harper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8614-3074","authenticated-orcid":true,"given":"John D.","family":"Boyce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"issue":"4","key":"ppat.1013398.ref001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0036067","article-title":"The canine oral microbiome","volume":"7","author":"FE Dewhirst","year":"2012","journal-title":"PLoS One"},{"key":"ppat.1013398.ref002","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1471-2180-12-20","article-title":"Defining the \u201ccore microbiome\u201d of the microbial communities in the tonsils of healthy pigs","volume":"12","author":"BA Lowe","year":"2012","journal-title":"BMC Microbiol"},{"issue":"2","key":"ppat.1013398.ref003","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/03079450094162","article-title":"Investigations on the carrier rate of Pasteurella multocida in healthy commercial poultry flocks and flocks affected by fowl 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