{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:33Z","timestamp":1787022213143,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013475","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000}}],"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32422085"],"award-info":[{"award-number":["32422085"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Program of China","award":["2022YFD1800200 and 2022YFD1800201"],"award-info":[{"award-number":["2022YFD1800200 and 2022YFD1800201"]}]},{"DOI":"10.13039\/501100004775","name":"the Natural Science Foundation of Gansu Province, China","doi-asserted-by":"crossref","award":["23JRRA1479 and 23JRRA555"],"award-info":[{"award-number":["23JRRA1479 and 23JRRA555"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004775","name":"the Natural Science Foundation of Gansu Province, China","doi-asserted-by":"crossref","award":["23JRRA553"],"award-info":[{"award-number":["23JRRA553"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Research Funding from the Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences","award":["CAAS-ASTIP-JBGS-20210801"],"award-info":[{"award-number":["CAAS-ASTIP-JBGS-20210801"]}]},{"name":"the Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"the Special Research Fund of Shanxi Agricultural University for High-level Talents","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    <\/jats:italic>\n                    is a significant pathogen in both humans and animals, with disease progression driven by the rapid proliferation of its tachyzoite stage. In this study, we identify the PP2A-2 holoenzyme as a key regulator of daughter cell emergence during parasite division. This holoenzyme, likely composed of the regulatory subunit TgPR48 (PP2A-B2), the catalytic subunit PP2A-C2, and the scaffolding subunit PP2A-A2, is essential for proper cytokinesis. Disruption of any single component severely impairs daughter cell separation and emergence. Phosphoproteomic analysis following PP2A-C2 depletion revealed numerous differentially phosphorylated proteins. Among these, DCS1 and DCS2 were prioritized as potential effectors. While phosphomimetic and non-phosphorylatable mutations in DCS1 and DCS2 did not significantly impair their function, depletion of either protein disrupted TgPR48 localization. Interestingly, TgPR48 overexpression partially rescued the phenotypes associated with DCS2 loss, but not DCS1, indicating divergence in their downstream pathways and implicating additional, yet unidentified, substrates. These findings establish PP2A-2\u2013mediated dephosphorylation as a central mechanism in regulating tachyzoite cytokinesis and highlight a promising regulatory axis for therapeutic intervention against\n                    <jats:italic>\n                      <jats:italic>T. gondii<\/jats:italic>\n                    <\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013475","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T17:59:43Z","timestamp":1756922383000},"page":"e1013475","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii"],"prefix":"10.1371","volume":"21","author":[{"given":"Jin-Lei","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian-Zhang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Ting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Jing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Quan","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany 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