{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:57:16Z","timestamp":1789243036762,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013727","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000}}],"reference-count":86,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002805","name":"Carl Tryggers Stiftelse f\u00c3\u00b6r Vetenskaplig Forskning","doi-asserted-by":"publisher","award":["CTS 22:1915"],"award-info":[{"award-number":["CTS 22:1915"]}],"id":[{"id":"10.13039\/501100002805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00c3\u00a5det","doi-asserted-by":"publisher","award":["2018-02223"],"award-info":[{"award-number":["2018-02223"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00c3\u00a5det","doi-asserted-by":"publisher","award":["2022-01590"],"award-info":[{"award-number":["2022-01590"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009252","name":"Science for Life Laboratory","doi-asserted-by":"publisher","award":["SciLifeLab Fellows program"],"award-info":[{"award-number":["SciLifeLab Fellows program"]}],"id":[{"id":"10.13039\/501100009252","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007067","name":"Kempestiftelserna","doi-asserted-by":"publisher","award":["JCK3126"],"award-info":[{"award-number":["JCK3126"]}],"id":[{"id":"10.13039\/501100007067","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The enteropathogen\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    employs a Type Three Secretion System (T3SS) to colonize intestinal epithelial cells. Genes encoding the T3SS are located on a large IncFII virulence plasmid, pINV. T3SS expression comes at the expense of slowed\n                    <jats:italic>Shigella<\/jats:italic>\n                    growth and is therefore strictly controlled by both transcriptional and post-transcriptional mechanisms. Following up on a recent genome-wide screen, we here show that the chromosomal gene\n                    <jats:italic>pcnB,<\/jats:italic>\n                    encoding the poly-A polymerase I (PAP-I), slows\n                    <jats:italic>Shigella<\/jats:italic>\n                    growth at 37\u00b0C, while it at the same time promotes early colonization of a human epithelial enteroid model. Proteomic profiling revealed that\n                    <jats:italic>pcnB<\/jats:italic>\n                    drives global increase of the\n                    <jats:italic>Shigella<\/jats:italic>\n                    T3SS virulence program. Accordingly,\n                    <jats:italic>pcnB<\/jats:italic>\n                    upholds pINV replication to a level favourable for\n                    <jats:italic>Shigella<\/jats:italic>\n                    virulence. This is achieved through increased degradation of the antisense RNA CopA, involved in plasmid replication control. The\n                    <jats:italic>pcnB<\/jats:italic>\n                    effect on pINV replication was found to also ensure longer-term intraepithelial expansion of\n                    <jats:italic>Shigella<\/jats:italic>\n                    following human intestinal epithelium invasion. Our findings exemplify how an adequate pINV level, sustained by\n                    <jats:italic>pcnB<\/jats:italic>\n                    , underpins the successful execution of\n                    <jats:italic>Shigella<\/jats:italic>\n                    \u00b4s infection cycle.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013727","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T18:37:51Z","timestamp":1763663871000},"page":"e1013727","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["The pcnB gene sustains Shigella flexneri virulence"],"prefix":"10.1371","volume":"21","author":[{"given":"Thibault","family":"Frisch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Geiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Komi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip 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Letizia","family":"Di Martino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"issue":"5","key":"ppat.1013727.ref001","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1093\/femsre\/fux023","article-title":"Evolution of bacterial virulence","volume":"41","author":"M Diard","year":"2017","journal-title":"FEMS Microbiol Rev"},{"issue":"17","key":"ppat.1013727.ref002","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2014.07.016","article-title":"Type III secretion system","volume":"24","author":"A Puhar","year":"2014","journal-title":"Curr Biol"},{"key":"ppat.1013727.ref003","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3389\/fcimb.2017.00064","article-title":"How Do the Virulence Factors of Shigella Work Together to Cause Disease?","volume":"7","author":"E Mattock","year":"2017","journal-title":"Front Cell Infect 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