{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:36:59Z","timestamp":1792114619862,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013812","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000}}],"reference-count":92,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICRAD","award":["862605 (Flu-Switch)"],"award-info":[{"award-number":["862605 (Flu-Switch)"]}]},{"name":"NWO","award":["TOP-PUNT 718.015.003"],"award-info":[{"award-number":["TOP-PUNT 718.015.003"]}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["J-4260-B21"],"award-info":[{"award-number":["J-4260-B21"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH","award":["75N93021C00015 \/ PENN CEIRR"],"award-info":[{"award-number":["75N93021C00015 \/ PENN CEIRR"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Highly pathogenic H5Ny influenza A viruses are causing unprecedented, season-independent outbreaks across avian and mammalian species, including dairy cattle, a novel reservoir. The sialoside-binding properties of influenza A hemagglutinin (HA) are strongly related to its ability to infect and transmit between hosts. Mucin-like O-glycans, omnipresent in respiratory tracts, have been understudied as viral receptors due to their complexity. To address this, we synthesized 25 O-linked glycans with diverse sialosides, including modifications by fucosides and sulfates. Our findings reveal that H5Ny 2.3.4.4b viruses bind core 3 sialyl-Lewis\n                    <jats:sup>x<\/jats:sup>\n                    and Sia-Gal-\u03b23GalNAc, O-linked glycans not recognized by classical H5 or other avian viruses. By determining crystal structures, we resolved the structural features of four glycans in an H5 hemagglutinin (HA) from a 2016 2.3.4.4b virus. While these viruses do not bind human-type receptors, their broad receptor specificity enhances binding to human tracheal tissues, suggesting that O-glycan recognition could contribute to the continues spillover of this clade.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013812","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T18:50:56Z","timestamp":1768935056000},"page":"e1013812","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["The receptor binding properties of H5Ny influenza A viruses have evolved to bind to avian-type mucin-like O-glycans"],"prefix":"10.1371","volume":"22","author":[{"given":"Julia","family":"Weber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels L. D.","family":"Ponse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"R\u00edos Carrasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvin X.","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathis","family":"Funk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Hui","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Gabarroca Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy 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Vries","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,1,20]]},"reference":[{"issue":"1","key":"ppat.1013812.ref001","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1111\/irv.12412","article-title":"The ecology and adaptive evolution of influenza A interspecies transmission","volume":"11","author":"U Joseph","year":"2017","journal-title":"Influenza Other Respir Viruses"},{"key":"ppat.1013812.ref002","first-page":"359","article-title":"Evolution and ecology of influenza A viruses","volume":"385","author":"S-W Yoon","year":"2014","journal-title":"Curr Top Microbiol Immunol"},{"issue":"1","key":"ppat.1013812.ref003","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1080\/22221751.2021.1872355","article-title":"Emergence and spread of novel H5N8, H5N5 and H5N1 clade 2.3.4.4 highly pathogenic avian influenza in 2020","volume":"10","author":"NS 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