{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:58:04Z","timestamp":1782867484938,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013821","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000}}],"reference-count":67,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI178725"],"award-info":[{"award-number":["R01AI178725"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Lyme disease is caused by the host-adapted spirochete\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    . With a genome of only 1.5 mbp,\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    is dependent on metabolites scavenged from their vertebrate and invertebrate hosts for growth. These scavenged nutrients include several lipid precursors: the spirochete is auxotrophic for fatty acids and cholesterol, and also accumulates environmental phospholipids. Comprehensive lipidomic analysis of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    by LC MS\/MS was used to identify previously undescribed membrane components. These include some likely scavenged from the culture medium and some which may be synthesized de novo via unknown pathways. Changes in fatty acid composition as cells enter stationary phase suggest that scavenging of environmental lipids is preferential to de novo synthesis, while transcriptomics suggests that this may be due to the energetic cost of synthesizing glycerol phosphate precursors. In media supplemented with excess phospholipids, scavenged lipids can be found at high concentrations in cells, suggesting that the membranes of infecting bacteria are likely to be partly shaped by the host environment. Transcriptomic analysis also show a link between environmental lipids and the expression of virulence-associated surface lipoproteins including reciprocal regulation of\n                    <jats:italic>ospA<\/jats:italic>\n                    and\n                    <jats:italic>ospC<\/jats:italic>\n                    . Given that borrelial membrane lipids are known to be antigenic during infection, these findings identify potential new targets for the development of diagnostic tests or vaccines.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013821","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T18:51:41Z","timestamp":1767034301000},"page":"e1013821","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Lipid scavenging by the Lyme disease spirochete Borrelia burgdorferi"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-5563","authenticated-orcid":true,"given":"Peter J.","family":"Gwynne","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery 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