{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T01:20:33Z","timestamp":1792286433759,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013922","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000}}],"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Netherlands Organisation for Scientific Research","doi-asserted-by":"crossref","award":["NWO-VIDI864.13.009"],"award-info":[{"award-number":["NWO-VIDI864.13.009"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006209","name":"Radboud Universitair Medisch Centrum","doi-asserted-by":"publisher","award":["Masters round 2018"],"award-info":[{"award-number":["Masters round 2018"]}],"id":[{"id":"10.13039\/501100006209","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006209","name":"Radboud Universitair Medisch Centrum","doi-asserted-by":"publisher","award":["Masters round 2021"],"award-info":[{"award-number":["Masters round 2021"]}],"id":[{"id":"10.13039\/501100006209","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006209","name":"Radboud University Medical Center","doi-asserted-by":"crossref","award":["RIMLS018-009b"],"award-info":[{"award-number":["RIMLS018-009b"]}],"id":[{"id":"10.13039\/501100006209","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Members of the\n                    <jats:underline>S<\/jats:underline>\n                    tomatin,\n                    <jats:underline>P<\/jats:underline>\n                    rohibitin,\n                    <jats:underline>F<\/jats:underline>\n                    lotillin and\n                    <jats:underline>H<\/jats:underline>\n                    flK\/C (SPFH) protein family form large membrane anchored or spanning complexes and are involved in various functions in different organelles. The human malaria causing parasite\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    harbors four SPFH proteins, including prohibitin 1 and 2, prohibitin-like protein (PHBL), and stomatin-like protein (STOML), which all localize to the parasite mitochondrion. In the murine model parasite\n                    <jats:italic>Plasmodium berghei<\/jats:italic>\n                    ,\n                    <jats:italic>STOML<\/jats:italic>\n                    appears essential for asexual blood-stage (ABS) development and is localized to puncta on mitochondrial branching points in oocyst stages. In this study, we show that deletion of\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    <jats:italic>STOML<\/jats:italic>\n                    causes a significant growth defect and slower ABS development, while sexual-stage development remains unaffected. Parasites lacking\n                    <jats:italic>STOML<\/jats:italic>\n                    were not more sensitive to respiratory chain targeting drugs, rendering a function of STOML in respiratory chain assembly unlikely. Epitope tagging of endogenous STOML revealed a distinct punctate localization on branching points and endings of the ABS mitochondrial network. STOML resides in a large protein complex and pulldown experiments identified a zinc dependent metalloprotease, FtsH, as a likely interaction partner. The predicted AlphaFold2 structure of STOML shows high similarity with the bacterial HflK\/C, which has been shown to form a large vault-like structure around bacterial FtsH hexamers. Combined, our results suggest that a similar STOML-FtsH complex localized to specific loci of\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    mitochondria facilitates the parasite\u2019s ABS development.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013922","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T18:50:59Z","timestamp":1770663059000},"page":"e1013922","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Plasmodium falciparum stomatin-like protein forms a putative complex with a metalloprotease in distinct mitochondrial loci"],"prefix":"10.1371","volume":"22","author":[{"given":"Julie M.J.","family":"Verhoef","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezra T.","family":"Bekkering","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cas","family":"Boshoven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Hannon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Evers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas I.","family":"Proellochs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia G.","family":"Spruijt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8547-7523","authenticated-orcid":true,"given":"Taco W. 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