{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:38:33Z","timestamp":1790307513747,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013933","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000}}],"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R01 AI178378, R01 AI169850 and R56 AI171920"],"award-info":[{"award-number":["R01 AI178378, R01 AI169850 and R56 AI171920"]}]},{"name":"NIH","award":["U19 AI181984"],"award-info":[{"award-number":["U19 AI181984"]}]},{"name":"NIH","award":["UC7 AI180311"],"award-info":[{"award-number":["UC7 AI180311"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Oropouche fever is a re-emerging global viral threat caused by infection with Oropouche virus (OROV). While disease is generally self-limiting, historical and recent reports of neurologic involvement highlight the importance of understanding the neuropathogenesis of OROV. In this study, we characterize viral replication kinetics in neurons, microglia, and astrocytes derived from immortalized, primary, and induced pluripotent stem cell-derived cells, which are all permissive to infection with the prototypic OROV BeAn19991. We demonstrate cell-type dependent replication kinetics with both historic and recently emerged viral strains. Further, we show that ex vivo rat brain slice cultures can be infected by all OROV strains and produce antiviral cytokines and chemokines, which introduces an additional model to study OROV kinetics and tropism in the central nervous system. These findings provide insight into OROV neuropathogenesis and an initial assessment of newly emerged strains.<\/jats:p>","DOI":"10.1371\/journal.ppat.1013933","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T18:40:14Z","timestamp":1770662414000},"page":"e1013933","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Neural cells are susceptible to historic and recently emerged Oropouche virus strains"],"prefix":"10.1371","volume":"22","author":[{"given":"Kaleigh A.","family":"Connors","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maris R.","family":"Pedlow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary D.","family":"Frey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjorie","family":"Cornejo Pontelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P. 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