{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:33:50Z","timestamp":1790062430018,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013948","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000}}],"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["220797\/Z\/20\/Z"],"award-info":[{"award-number":["220797\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["218482\/Z\/19\/Z"],"award-info":[{"award-number":["218482\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Intestinal amoebiasis is caused by\n                    <jats:italic>Entamoeba histolytica,<\/jats:italic>\n                    one of the deadliest human-infective parasites. Central to its pathogenicity is its binding to mucosal carbohydrates, which precedes tissue damage by trogocytosis. Carbohydrate binding is mediated by a single adhesin, the galactose\/N-acetylgalactosamine (Gal\/GalNAc) lectin, which is the leading vaccine candidate for amoebiasis. We present the structure of the native heterodimeric lectin, revealing an ordered core containing the light chain and the N-terminal region of the heavy chain. Structures obtained in the presence of ligand show that the Gal\/GalNAc binding site is in the light chain, which adopts a \u03b2-trefoil fold found in other lectins. An elongated arm emerges from the heavy chain, which adopts multiple positions and may be modulated by sugar binding. This study reveals the molecular basis for sugar binding by the\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    Gal\/GalNAc lectin, a prerequisite for parasite invasion and development of intestinal disease.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013948","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T18:40:10Z","timestamp":1771958410000},"page":"e1013948","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural basis for carbohydrate recognition by the Gal\/GalNAc lectin of Entamoeba histolytica involved in host cell adhesion"],"prefix":"10.1371","volume":"22","author":[{"given":"Samuel F.","family":"G\u00e9rard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Redfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2870-1955","authenticated-orcid":true,"given":"Matthew K.","family":"Higgins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"issue":"9859","key":"ppat.1013948.ref001","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"R Lozano","year":"2012","journal-title":"Lancet"},{"issue":"7","key":"ppat.1013948.ref002","article-title":"A Review of the Global Burden, New Diagnostics, and Current Therapeutics for Amebiasis","volume":"5","author":"D-AT Shirley","year":"2018","journal-title":"Open Forum Infect Dis"},{"key":"ppat.1013948.ref003","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1056\/NEJMra022710","article-title":"Amebiasis","volume":"348","author":"R Haque","year":"2003","journal-title":"New England J Med"},{"issue":"3","key":"ppat.1013948.ref004","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0046-8177(70)80072-7","article-title":"Pathology of human amebiasis","volume":"1","author":"H Brandt","year":"1970","journal-title":"Hum Pathol"},{"key":"ppat.1013948.ref005","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev.micro.56.012302.160959","article-title":"The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica","volume":"56","author":"WA Petri","year":"2002","journal-title":"Annu Rev Microbiol"},{"issue":"5","key":"ppat.1013948.ref006","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1016\/S0021-9258(19)81714-3","article-title":"Subunit structure of the galactose and N-acetyl-D-galactosamine-inhibitable adherence lectin of Entamoeba histolytica","volume":"264","author":"WA Petri","year":"1989","journal-title":"J Biol Chem"},{"issue":"5","key":"ppat.1013948.ref007","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1093\/infdis\/151.5.804","article-title":"N-Acetyl-D-galactosamine-inhibitable adherence lectin of Entamoeba histolytica. 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