{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:49:34Z","timestamp":1782949774032,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.ppat.1013992","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000}}],"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX62-IBEID"],"award-info":[{"award-number":["ANR-10-LABX62-IBEID"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010662","name":"H2020 Excellent Science","doi-asserted-by":"publisher","award":["101080309"],"award-info":[{"award-number":["101080309"]}],"id":[{"id":"10.13039\/100010662","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The currently licensed anti-tuberculosis (TB) vaccine,\n                    <jats:italic>Mycobacterium bovis<\/jats:italic>\n                    BCG, provides limited protection against pulmonary TB in adolescents and adults, the main cause of TB transmission and mortality. To obtain an improved BCG-based vaccine candidate with increased immune signalling but still low virulence, we have previously generated a recombinant BCG strain named BCG::ESX-1\n                    <jats:sup>\n                      <jats:italic>Mmar<\/jats:italic>\n                    <\/jats:sup>\n                    , which is heterologously expressing ESX-1 functions of\n                    <jats:italic>Mycobacterium marinum<\/jats:italic>\n                    and thereby modulates the host innate immune responses via phagosomal rupture-associated induction of type I interferon production and enhanced inflammasome activity, leading to superior protection against TB disease in murine infection models. As protection may also vary with the route of vaccination, here, we have explored aerosol vaccination relative to subcutaneous vaccination, using BCG Pasteur and BCG::ESX-1\n                    <jats:sup>\n                      <jats:italic>Mmar<\/jats:italic>\n                    <\/jats:sup>\n                    . We found that mice vaccinated via the aerosol route with BCG Pasteur or BCG::ESX-1\n                    <jats:sup>\n                      <jats:italic>Mmar<\/jats:italic>\n                    <\/jats:sup>\n                    both yielded higher frequencies of CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    Th1 activated effectors and T effector memory cells in the lungs compared to subcutaneously immunised mice, whereas comparable polyfunctional Th1 (IL-2, TNF-\u03b1 and IFN-\u03b3) cytokine-producing subsets were observed in the spleens of all vaccinated mice. Significantly higher IL-17A responses without severe lung pathology were seen in the lungs of aerosol-vaccinated mice associated to local and transient inflammatory cytokine responses and immune cell infiltrations. In contrast to the subcutaneous route, aerosol vaccination elicited high amounts of humoral IgG and IgA responses in the bronchoalveolar lavage fluid and induced a substantial number of lung CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells expressing CD69\n                    <jats:sup>+<\/jats:sup>\n                    CD103\n                    <jats:sup>+<\/jats:sup>\n                    tissue-residency markers. These effects led to significant improved protection against\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    and reduced lung pathology in aerosol-vaccinated mice compared to subcutaneously vaccinated mice. Moreover, BCG::ESX-1\n                    <jats:sup>\n                      <jats:italic>Mmar<\/jats:italic>\n                    <\/jats:sup>\n                    vaccine induced enhanced T-cell immunity and superior protection compared to parental BCG Pasteur for both vaccination routes and thereby represents an interesting candidate for developing improved vaccination strategies against TB.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1013992","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T18:54:31Z","timestamp":1771872871000},"page":"e1013992","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Improved immune responses and tuberculosis protection by aerosol vaccination with recombinant BCG expressing ESX-1 from Mycobacterium marinum"],"prefix":"10.1371","volume":"22","author":[{"given":"Fadel","family":"Sayes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wafa","family":"Frigui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Pawlik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Tillier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Tichit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Hardy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2587-3863","authenticated-orcid":true,"given":"Roland","family":"Brosch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"ppat.1013992.ref001","unstructured":"WHO. 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