{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:06:55Z","timestamp":1789063615088,"version":"build-2803163510"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Newly emerging influenza viruses pose a constant threat as they encounter a population lacking neutralizing antibodies against the new strain. However, cross-reactive non-neutralizing antibodies (nnABs) may be present and help mitigate disease symptoms through various effector mechanisms, including antibody-dependent cellular cytotoxicity (ADCC). Although nnABs to influenza virus have received more attention lately, little information is available on their age-related prevalence, steady-state levels, functional properties, and changes in these parameters over time. Using longitudinal samples from adolescents, adults, and older adults, collected before and after the 2009 swine flu pandemic, we comprehensively characterized the specificity and functionality of nnAB responses against H1N1 pandemic 2009 (H1N1pdm09) virus. Remarkably, all participants exhibited cross-reactive antibodies to this virus before having encountered it through infection or vaccination, with the highest baseline levels observed in older adults. The levels of these IgG antibodies showed a strong correlation with engagement of fragment crystallizable \u03b3 receptor IIIa (Fc\u03b3RIIIa) and NK cell activation, both of which were notably lower in adolescents than in adults and older adults. Without infection or vaccination, average amounts of H1N1pdm09-reactive antibodies remained relatively stable on the population level over the 5-year study period. However, on an individual level, substantial increases and decreases occurred. H1N1pdm09 infection or vaccination significantly enhanced specific antibody levels and the Fc\u03b3RIIIa-engaging capacity of these antibodies in all age groups. ADCC-mediating antibodies increased, however, only in adolescents, reaching the same level as observed in the adult groups. Taken together, our results demonstrate the presence of cross-reactive, non-neutralizing, functional, and boostable antibodies against a never-encountered influenza virus strain across all age groups. These antibodies might contribute to protection from severe disease. Accordingly, in case of a newly emerging influenza virus, their further enhancement by vaccination could be beneficial as an immediate protective measure before a strain-specific vaccine becomes available.<\/jats:p>","DOI":"10.1371\/journal.ppat.1014014","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:21:37Z","timestamp":1789060897000},"page":"e1014014","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal assessment of functional antibodies to a novel influenza virus strain across age groups"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2646-5143","authenticated-orcid":true,"given":"Anke","family":"Huckriede","sequence":"first","affiliation":[{"name":"University Medical Center Groningen, University of Groningen","place":["Groningen, The Netherlands"],"department":["Department of Medical Microbiology and Infection Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Hoorn","sequence":"additional","affiliation":[{"name":"University Medical Center Groningen, University of Groningen","place":["Groningen, The Netherlands"],"department":["Department of Medical Microbiology and Infection Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manas","family":"Joshi","sequence":"additional","affiliation":[{"name":"University Medical Center Groningen, University of Groningen","place":["Groningen, The Netherlands"],"department":["Department of Medical Microbiology and Infection Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"de Vries-Idema","sequence":"additional","affiliation":[{"name":"University Medical Center Groningen, University of Groningen","place":["Groningen, The Netherlands"],"department":["Department of Medical Microbiology and Infection Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gestur","family":"Vidarsson","sequence":"additional","affiliation":[{"name":"Sanquin Research","place":["Amsterdam, The Netherlands"],"department":["Immunoglobulin Research Laboratory"]},{"name":"Utrecht University","place":["Utrecht, The Netherlands"],"department":["Department of Pharmaceutical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puck B.","family":"van Kasteren","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM)","place":["Bilthoven, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Beukema","sequence":"additional","affiliation":[{"name":"University Medical Center Groningen, University of Groningen","place":["Groningen, The Netherlands"],"department":["Department of Medical Microbiology and Infection Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"ppat.1014014.ref001","unstructured":"Avian Influenza Weekly Update # 1007. 2025. 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