{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:11:31Z","timestamp":1790014291242,"version":"4.0.1"},"reference-count":80,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_184666"],"award-info":[{"award-number":["310030_184666"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF19SA0059362"],"award-info":[{"award-number":["NNF19SA0059362"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The plant-beneficial bacterium\n                    <jats:italic>Pseudomonas protegens<\/jats:italic>\n                    CHA0 (CHA0) is widely studied for the biological control of soil-borne plant diseases. Beyond its root-colonising capabilities, CHA0 can also infect and kill insect larvae and thus exhibits a multi-host lifestyle shared with other plant- and insect-colonising bacteria. To better understand the robustness of this multi-host lifestyle, we subjected CHA0 to ten consecutive passages through larvae of the pest insect\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    via repeated cycles of insect colonisation and killing forcing it into an insect-only lifestyle. Overall, serial passaging did not result in consistent changes in insect killing speed, larval or root colonisation, plant protection efficiency, microbial antagonism or\n                    <jats:italic>in vitro<\/jats:italic>\n                    growth. This suggests that its multi-host lifestyle was conserved following serial passage. Nonetheless, a few independently passaged lines showed an increase in larval killing speed, which in one case might be linked to choline uptake. To disentangle changes specific to the insect host from those arising due to the experimental system itself, we conducted parallel serial passages through the same system while omitting the insect host. In some of these lines, exposure to the background of the system led to changes in microbial antagonism and in\n                    <jats:italic>in vitro<\/jats:italic>\n                    growth, which likely are associated with mutations in regions encoding for regulatory systems. Our findings indicate that\n                    <jats:italic>P. protegens<\/jats:italic>\n                    CHA0 remains phenotypically stable in complex environments such as an insect host, suggesting that the multi-host lifestyle might also be conserved when applied in the field and supporting CHA0\u2019s potential for reliable biocontrol performance against both plant diseases and insect pests.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014101","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:50:53Z","timestamp":1790013053000},"page":"e1014101","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The insect- and plant-associated lifestyles of Pseudomonas protegens CHA0 are preserved following serial passage through insect larvae"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3542-1465","authenticated-orcid":true,"given":"Maria","family":"Zwyssig","sequence":"first","affiliation":[{"name":"Plant Pathology, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH) Zurich","place":["Zurich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Schneider","sequence":"additional","affiliation":[{"name":"Plant Pathology, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH) Zurich","place":["Zurich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gijs","family":"Selten","sequence":"additional","affiliation":[{"name":"Science for Life, Utrecht University","place":["Utrecht, The Netherlands"],"department":["Plant-Microbe Interactions, Department of Biology"]},{"name":"Science for Life, Utrecht University","place":["Utrecht, The Netherlands"],"department":["AI Technologies for Life, Department of Information and Computing Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Keel","sequence":"additional","affiliation":[{"name":"University of Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Fundamental Microbiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Maurhofer","sequence":"additional","affiliation":[{"name":"Plant Pathology, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH) Zurich","place":["Zurich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronnie","family":"de Jonge","sequence":"additional","affiliation":[{"name":"Science for Life, Utrecht University","place":["Utrecht, The Netherlands"],"department":["Plant-Microbe Interactions, Department of Biology"]},{"name":"Science for Life, Utrecht University","place":["Utrecht, The Netherlands"],"department":["AI Technologies for Life, Department of Information and Computing Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"ppat.1014101.ref001","first-page":"100084","article-title":"Pseudomonas mediated nutritional and growth promotional activities for sustainable food security","volume":"2","author":"S Sah","year":"2021","journal-title":"Curr Res Microb Sci"},{"key":"ppat.1014101.ref002","doi-asserted-by":"crossref","first-page":"104538","DOI":"10.1016\/j.biocontrol.2021.104538","article-title":"Exploiting the biocontrol potential of plant-associated pseudomonads \u2013 A step towards pesticide-free agriculture?","volume":"155","author":"T M\u00fcller","year":"2021","journal-title":"Biological Control"},{"key":"ppat.1014101.ref003","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3389\/fpls.2013.00287","article-title":"Promise for plant pest control: root-associated pseudomonads with insecticidal 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