{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T18:04:07Z","timestamp":1784225047473,"version":"3.55.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2025YFD1800803"],"award-info":[{"award-number":["2025YFD1800803"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2021YFD1800403"],"award-info":[{"award-number":["2021YFD1800403"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2021YFC2600204"],"award-info":[{"award-number":["2021YFC2600204"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Agricultural Core Technology Project of Hubei Province","award":["HBNYHXGG2023-3,NYWSWZX2025-2027-03"],"award-info":[{"award-number":["HBNYHXGG2023-3,NYWSWZX2025-2027-03"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The escalating crisis of antibiotics against Gram negative bacteria resistance demands innovative strategies to combat recalcitrant intracellular pathogens. We reposition RS 17053, an \u03b11A-adrenoceptor antagonist with unexplored antibacterial function, as a mechanistically distinct broad-spectrum antibiotic. We proved RS 17053 has strong binding ability with Class A penicillin binding proteins (PBPs), which overcomes the \u03b2-lactam barrier by penetrating host cells and disrupts the biosynthesis of peptidoglycan by uniquely targeting MrcA\/MecA. RS 17053 eradicated Gram-negative (\n                    <jats:italic>Brucella<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella<\/jats:italic>\n                    ) and Gram-positive (Methicillin resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , MRSA) pathogens\n                    <jats:italic>in vitro<\/jats:italic>\n                    (MICs 0.25\u20134 \u03bcg\/mL) and eliminated intracellular\n                    <jats:italic>Brucella<\/jats:italic>\n                    within macrophages at 8 \u03bcg\/mL. Crucially, upon serial passaging under drug pressure, RS 17053 showed a slower progression to high-level resistance. In murine zoonotic infection models, 10 mg\/kg\/day RS 17053 achieved higher survival rate by caused a 2-log CFU\/mL reduction in organs and normalizing pro-inflammatory cytokines. Multi-omics and phenotypic analyses confirmed selective PBP targeting, causing peptidoglycan collapse and bacterial lysis. This work has provided a promising therapeutic against intractable intracellular infections.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014242","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T17:24:15Z","timestamp":1784222655000},"page":"e1014242","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel penicillin-binding protein inhibitor with unprecedented intracellular activity eradicates multiple pathogenic 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Kahl-McDonagh","year":"2006","journal-title":"Infect Immun"},{"key":"ppat.1014242.ref025","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1093\/ilar.38.1.41","article-title":"Special Report: The 1996 Guide for the Care and Use of Laboratory Animals","volume":"38","author":"JD Clark","year":"1997","journal-title":"ILAR Journal"},{"key":"ppat.1014242.ref026","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-60761-247-6_1","article-title":"Modes of defining atherosclerosis in mouse models: relative merits and evolving standards","volume":"573","author":"A Daugherty","year":"2009","journal-title":"Methods Mol Biol"},{"issue":"4","key":"ppat.1014242.ref027","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.03000-23","article-title":"Distribution and genetic characterization of fluoroquinolone resistance gene qnr among Salmonella strains from chicken in China","volume":"12","author":"Y Chen","year":"2024","journal-title":"Microbiol 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Staphylococcus aureus (MRSA)","volume":"28","author":"SS Ambade","year":"2023","journal-title":"Molecules"},{"issue":"2","key":"ppat.1014242.ref031","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/j.1574-6976.2008.00105.x","article-title":"The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis","volume":"32","author":"E Sauvage","year":"2008","journal-title":"FEMS Microbiol Rev"},{"issue":"2","key":"ppat.1014242.ref032","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro2677","article-title":"From the regulation of peptidoglycan synthesis to bacterial growth and morphology","volume":"10","author":"A Typas","year":"2011","journal-title":"Nat Rev Microbiol"},{"issue":"12","key":"ppat.1014242.ref033","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"MP Franco","year":"2007","journal-title":"Lancet Infect 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regulators","volume":"183","author":"TK McKinney","year":"2001","journal-title":"J Bacteriol"},{"issue":"9","key":"ppat.1014242.ref040","doi-asserted-by":"crossref","first-page":"3664","DOI":"10.1021\/ja500053x","article-title":"Discovery of a new class of non-\u03b2-lactam inhibitors of penicillin-binding proteins with Gram-positive antibacterial activity","volume":"136","author":"PI O\u2019Daniel","year":"2014","journal-title":"J Am Chem Soc"},{"issue":"2","key":"ppat.1014242.ref041","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1574-6976.2007.00094.x","article-title":"Peptidoglycan structure and architecture","volume":"32","author":"W Vollmer","year":"2008","journal-title":"FEMS Microbiol Rev"},{"issue":"11","key":"ppat.1014242.ref042","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1021\/acs.accounts.5c00113","article-title":"Decoding the Penicillin-Binding Proteins with Activity-Based Probes","volume":"58","author":"EE Carlson","year":"2025","journal-title":"Acc Chem Res"},{"key":"ppat.1014242.ref043","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3389\/fchem.2019.00043","article-title":"Reassessing the Host Defense Peptide Landscape","volume":"7","author":"EF Haney","year":"2019","journal-title":"Front Chem"},{"issue":"2","key":"ppat.1014242.ref044","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"G Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"issue":"3","key":"ppat.1014242.ref045","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"E Tacconelli","year":"2018","journal-title":"Lancet Infect 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