{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T18:02:04Z","timestamp":1781028124324,"version":"3.54.1"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Health Training Project of High-level Talents","award":["L-2019023"],"award-info":[{"award-number":["L-2019023"]}]},{"name":"Eminent Doctors Program of Yunnan Province","award":["KH-SWR-MY-2020-005"],"award-info":[{"award-number":["KH-SWR-MY-2020-005"]}]},{"name":"Yunnan Major Scientific and Technological Projects","award":["202302AA310044-01"],"award-info":[{"award-number":["202302AA310044-01"]}]},{"name":"Yunnan Provincial Young and Middle-aged Academic and Technical Leaders Reserve Talents Program","award":["202105AC160029"],"award-info":[{"award-number":["202105AC160029"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor strongly associated with Epstein-Barr virus (EBV) infection. EBV-mediated dysregulation of host microRNAs (miRNAs) contributes to NPC pathogenesis, but the functions of many EBV-regulated host miRNAs remain incompletely defined. miR-10b-3p is markedly downregulated in EBV-positive NPC, yet its biological significance and downstream mechanism remain unclear. Here, we found that miR-10b-3p was reduced in EBV-positive NPC tissues and was further suppressed following EBV infection of non-malignant nasopharyngeal epithelial cells and EBV-negative NPC cell lines. Restoration of miR-10b-3p expression markedly inhibited cell proliferation, colony formation, migration, invasion, and epithelial-mesenchymal transition (EMT) in EBV-positive NPC cells, whereas inhibition of miR-10b-3p in EBV-negative NPC cells produced the opposite effects. In nude mouse xenograft and lung metastasis models, overexpression of miR-10b-3p significantly reduced tumor growth and pulmonary metastasis. Mechanistically, miR-10b-3p directly targeted the 3\u2032-UTR of integrin subunit alpha V (\n                    <jats:italic>ITGAV<\/jats:italic>\n                    ), leading to decreased ITGAV expression and subsequent attenuation of STAT5 and ERK1\/2 signaling. Forced ITGAV expression partially reversed the suppressive effects of miR-10b-3p on tumor cell proliferation, migration, invasion, and EMT. Moreover, miR-10b-3p levels were inversely correlated with ITGAV expression in NPC tissues. Collectively, these findings identify an EBV-regulated miR-10b-3p\/ITGAV\/STAT5-ERK1\/2 axis in NPC and show that loss of miR-10b-3p promotes tumor growth and metastasis by relieving\n                    <jats:italic>ITGAV<\/jats:italic>\n                    repression, suggesting potential therapeutic targets for EBV-associated NPC.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014304","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T17:34:51Z","timestamp":1781026491000},"page":"e1014304","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Downregulation of miR-10b-3p by EBV promotes tumor growth and metastasis via ITGAV in nasopharyngeal carcinoma"],"prefix":"10.1371","volume":"22","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6076-7711","authenticated-orcid":true,"given":"Yaoqiang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingdong","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqin","family":"Dian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"issue":"10192","key":"ppat.1014304.ref001","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0140-6736(19)30956-0","article-title":"Nasopharyngeal 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Jiromaru","year":"2022","journal-title":"Cancer Manag Res"},{"issue":"1","key":"ppat.1014304.ref005","doi-asserted-by":"crossref","first-page":"29715","DOI":"10.1038\/s41598-025-14455-5","article-title":"Systemic immune-inflammation index and serum lactate dehydrogenase predict the prognosis of non-metastatic nasopharyngeal carcinoma patients receiving intensity-modulated radiotherapy","volume":"15","author":"C Zhang","year":"2025","journal-title":"Sci Rep"},{"issue":"1","key":"ppat.1014304.ref006","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1186\/s12967-025-06549-5","article-title":"EBV Reactivation-associated gene signature predicts poor prognosis in nasopharyngeal carcinoma","volume":"23","author":"Q Luo","year":"2025","journal-title":"J Transl Med"},{"issue":"10","key":"ppat.1014304.ref007","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.1002\/ijc.31967","article-title":"Vesicle-bound EBV-BART13-3p miRNA in circulation distinguishes nasopharyngeal from 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