{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:50:46Z","timestamp":1782237046803,"version":"3.54.5"},"reference-count":100,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI147527"],"award-info":[{"award-number":["AI147527"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI059722"],"award-info":[{"award-number":["AI059722"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI059722"],"award-info":[{"award-number":["AI059722"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK020572"],"award-info":[{"award-number":["DK020572"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Proteus mirabilis<\/jats:italic>\n                    is a leading cause of complicated urinary tract infections (UTIs). Prior work showed\n                    <jats:italic>P. mirabilis<\/jats:italic>\n                    metabolizes sugars during experimental UTI, yet the role of sugar import systems in pathogenesis remains poorly defined. To investigate this, we generated a panel of 47 targeted mutants in predicted sugar transporter genes and assessed their growth\n                    <jats:italic>in vitro<\/jats:italic>\n                    and fitness\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Growth screening in nutrient-rich and minimal media revealed carbon source-dependent defects in several phosphotransferase system (PTS) mutants, including\n                    <jats:italic>ptsH<\/jats:italic>\n                    and\n                    <jats:italic>ptsI<\/jats:italic>\n                    . Pooled insertion sequencing (In-seq) identified\n                    <jats:italic>xapB<\/jats:italic>\n                    ,\n                    <jats:italic>ptsH<\/jats:italic>\n                    , and\n                    <jats:italic>ptsI<\/jats:italic>\n                    as\n                    <jats:italic>in vivo<\/jats:italic>\n                    fitness factors, with validation in a traditional murine co-challenge model. Functional studies showed that\n                    <jats:italic>xapB<\/jats:italic>\n                    , annotated as a xanthosine permease, did not support xanthosine or guanosine uptake in\n                    <jats:italic>P. mirabilis<\/jats:italic>\n                    , suggesting misannotation. Dissection of the PTS network revealed that a triple mutant lacking\n                    <jats:italic>scrA<\/jats:italic>\n                    ,\n                    <jats:italic>ulaC<\/jats:italic>\n                    , and\n                    <jats:italic>ptsG<\/jats:italic>\n                    recapitulated the\n                    <jats:italic>ptsH<\/jats:italic>\n                    phenotype\n                    <jats:italic>in vivo<\/jats:italic>\n                    . To evaluate whether increased sugar availability exacerbates these defects, we modeled glucosuria using the SGLT2 inhibitor dapagliflozin in CBA\/J mice. Dapagliflozin treatment significantly increased urinary glucose and enhanced\n                    <jats:italic>P. mirabilis<\/jats:italic>\n                    colonization. There was an inverse correlation between colonization and urinary glucose, but only in untreated mice. These findings reveal limitations in genome-based transporter annotation, establish a functional link between sugar import and\n                    <jats:italic>P. mirabilis<\/jats:italic>\n                    fitness during UTI, and demonstrate that host metabolic conditions such as glucosuria can influence the severity of infection.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014324","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:27:37Z","timestamp":1782235657000},"page":"e1014324","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective sugar transport supports Proteus mirabilis fitness in the urinary tract"],"prefix":"10.1371","volume":"22","author":[{"given":"Allyson E.","family":"Shea","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiuhyang","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surbhi","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara N.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trishna","family":"Appaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry L. T.","family":"Mobley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4553-3276","authenticated-orcid":true,"given":"Melanie M.","family":"Pearson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"issue":"1","key":"ppat.1014324.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.idc.2013.09.003","article-title":"Urinary tract infection syndromes: occurrence, recurrence, bacteriology, risk factors, and disease burden","volume":"28","author":"B Foxman","year":"2014","journal-title":"Infect Dis Clin North Am"},{"issue":"2","key":"ppat.1014324.ref002","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1067\/mda.2003.7","article-title":"Epidemiology of urinary tract infections: incidence, morbidity, and economic costs","volume":"49","author":"B Foxman","year":"2003","journal-title":"Dis Mon"},{"issue":"6","key":"ppat.1014324.ref003","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1093\/infdis\/146.6.719","article-title":"A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters","volume":"146","author":"JW Warren","year":"1982","journal-title":"J Infect Dis"},{"issue":"2","key":"ppat.1014324.ref004","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/jgs.14533","article-title":"How Often Do Clinically Diagnosed Catheter-Associated Urinary Tract Infections in Nursing Homes Meet Standardized Criteria?","volume":"65","author":"CE Armbruster","year":"2017","journal-title":"J Am Geriatr Soc"},{"issue":"5","key":"ppat.1014324.ref005","first-page":"346","article-title":"Urease. 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