{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T18:06:34Z","timestamp":1784052394279,"version":"3.55.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["Nos. 32373028"],"award-info":[{"award-number":["Nos. 32373028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171300"],"award-info":[{"award-number":["32171300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Open Project Program of Jiangsu Key Laboratory of Zoonosis","award":["No. R2405"],"award-info":[{"award-number":["No. R2405"]}]},{"name":"Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS-CSLPDCP-202402"],"award-info":[{"award-number":["CAAS-CSLPDCP-202402"]}]},{"name":"grants from the Joint-Foundation of Gansu province","award":["23JRRA1515"],"award-info":[{"award-number":["23JRRA1515"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Foot-and-mouth disease virus (FMDV) exhibits extensive antigenic diversity and limited cross-protection among different serotypes, making the identification of broadly neutralizing antibodies (bnAbs) critical for the development of vaccines that confer broad and effective protection. Here, we isolated four porcine-derived bnAbs that broadly neutralized multiple representative strains from FMDV serotypes O and A. These bnAbs convergently target a conserved antigenic region, with residue 204 (K) towards the VP1 C-terminus serving as a key determinant. Notably, the bnAb pO18-10, which competes with antibodies recognizing the VP1 G-H loop, VP1 C-terminus, and VP3 epitopes, exhibits the broadest neutralization breadth, including cross-neutralization against serotype Asia1. Cryo-electron microscopy (cryo-EM) of pO18-10 in complex with FMDV serotype O (O\/18074) revealed a novel cross-protomer antigenic region located around the icosahedral five-fold axis of the viral capsid, with an interaction footprint of up to 1452 \u00c5\u00b2. pO18-10 engages this epitope through interactions with the VP1 C-terminus and VP3 G-H loop of one protomer, as well as the VP1 E-F loop of an adjacent protomer, demonstrating extensive antigenic coverage. Residues 204 (K) and 210 (K) on VP1 were identified as key determinants of this cross-serotype antigenic site. Furthermore, pO18-10 sterically blocks the integrin receptor \u03b1v\u03b26, effectively neutralizing FMDV by preventing viral attachment.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , pO18-10 conferred robust prophylactic and therapeutic protection against FMDV-induced mortality and clinical disease in mice. Collectively, these findings provide a promising neutralizing antibody candidate for immunodiagnostic applications and define a cross-serotype antigenic structure for universal FMDV vaccine design.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014357","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T17:22:02Z","timestamp":1784049722000},"page":"e1014357","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural basis of a conserved, broad antigenic region surrounding the five-fold axis of foot-and-mouth disease virus revealed by swine neutralizing antibodies"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4934-4738","authenticated-orcid":true,"given":"Qiongqiong","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanquan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lv","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanhui","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinghua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimei","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifang","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingwen","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixun","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jijun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1412-5069","authenticated-orcid":true,"given":"Xiangmin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9138-692X","authenticated-orcid":true,"given":"Zengjun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1640-0693","authenticated-orcid":true,"given":"Dongsheng","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8307-634X","authenticated-orcid":true,"given":"Kun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"issue":"7","key":"ppat.1014357.ref001","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1136\/vr.160.7.238","article-title":"Poverty 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