{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:39:01Z","timestamp":1790282341477,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1014374","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000}}],"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/T016159\/1"],"award-info":[{"award-number":["MR\/T016159\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V007467\/1"],"award-info":[{"award-number":["BB\/V007467\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MICIU\/AEI","award":["PID2023-149259NB-I00"],"award-info":[{"award-number":["PID2023-149259NB-I00"]}]},{"name":"ERDF\/EU","award":["Support associated with PID2023-149259NB-I00"],"award-info":[{"award-number":["Support associated with PID2023-149259NB-I00"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The\n                    <jats:italic>Orthobunyavirus<\/jats:italic>\n                    genus of arthropod-borne segmented RNA viruses comprises important pathogens including the human-infecting Oropouche virus and ruminant-infecting Schmallenberg virus (SBV). The prototypical Bunyamwera orthobunyavirus (BUNV) possesses envelope-embedded glycoprotein Gn-Gc tripodal spikes, of which the ectodomains mediate virus entry, while endodomains interact with nucleoprotein (NP) enwrapped genome segments driving virion assembly. Interestingly, BUNV Gc head\/stalk domains are redundant for virus growth in mammalian cells, consistent with isolations of SBV from ruminants bearing head\/stalk deletions. However, these domains appear strictly maintained in orthobunyavirus isolations from arthropods in nature. To investigate the molecular mechanism that underlines this discrepancy, we compared the multiplication characteristics of wildtype BUNV (BUNV-WT) with a Gc head\/stalk deleted BUNV (BUNV-\u22067). In mammalian cells BUNV-WT and BUNV-\u22067 grew to equivalent titres, whereas in insect cells BUNV-\u22067 titres were 1000-fold lower and strikingly produced no virions following blood meal infection of\n                    <jats:italic>Aedes<\/jats:italic>\n                    mosquitoes. To understand this insect-specific restriction in virion production, we showed the intracellular abundance of BUNV-WT and BUNV-\u22067 Gc and NP components were equivalent, suggesting the deletion impacted post-translational stages of the infection cycle. To explore this, we investigated Gc and \u22067-Gc interactions during BUNV-WT and BUNV-\u22067 infections of both insect and mammalian cells by co-immunoprecipitation and multiplex mass spectrometry, revealing \u22067-Gc exhibited markedly reduced NP interactions in insect cells, potentially indicating reduced segment interactions during assembly. We hypothesize that the Gc head\/stalk performs an insect cell-specific role in segment recruitment during virion formation, and that maintenance in nature of full-length Gc is due to this essential role in the insect host.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014374","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:39:20Z","timestamp":1783445960000},"page":"e1014374","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Bunyamwera orthobunyavirus Gc glycoprotein head and stalk drives an infectious virion assembly pathway specific for the insect host"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6945-6124","authenticated-orcid":true,"given":"Amelia B.","family":"Shaw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiu Nam","family":"Tse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly V.","family":"Durawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owen","family":"Byford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley M.","family":"Pearson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth A.","family":"Stapleford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Fontana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9035-2039","authenticated-orcid":true,"given":"John N.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"issue":"9","key":"ppat.1014374.ref001","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.3390\/v13091702","article-title":"Mammals Preferred: Reassortment of Batai and Bunyamwera orthobunyavirus Occurs in Mammalian but Not Insect Cells","volume":"13","author":"A Heitmann","year":"2021","journal-title":"Viruses"},{"key":"ppat.1014374.ref002","doi-asserted-by":"crossref","first-page":"107295","DOI":"10.1016\/j.ijid.2024.107295","article-title":"Oropouche virus infection: Differential clinical outcomes and emerging global concerns of vertical transmission and fatal cases","volume":"150","author":"G Ceccarelli","year":"2025","journal-title":"Int J Infect 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Virol"},{"issue":"1","key":"ppat.1014374.ref042","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1128\/JVI.73.1.251-259.1999","article-title":"Generation of Bovine Respiratory Syncytial Virus (BRSV) from cDNA: BRSV NS2 Is Not Essential for Virus Replication in Tissue Culture, and the Human RSV Leader Region Acts as a Functional BRSV Genome Promoter","volume":"73","author":"UJ Buchholz","year":"1999","journal-title":"J Virol"},{"issue":"26","key":"ppat.1014374.ref043","doi-asserted-by":"crossref","first-page":"15400","DOI":"10.1073\/pnas.93.26.15400","article-title":"Rescue of a segmented negative-strand RNA virus entirely from cloned complementary DNAs","volume":"93","author":"A Bridgen","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ppat.1014374.ref044","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1083\/jcb.9.2.409","article-title":"Improvements in epoxy resin embedding methods","volume":"9","author":"JH Luft","year":"1961","journal-title":"J Biophys Biochem Cytol"},{"issue":"1","key":"ppat.1014374.ref045","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1083\/jcb.17.1.208","article-title":"The use of lead citrate at high pH as an electron-opaque stain in electron microscopy","volume":"17","author":"ES Reynolds","year":"1963","journal-title":"J Cell Biol"},{"issue":"7","key":"ppat.1014374.ref046","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nat Methods"},{"issue":"22","key":"ppat.1014374.ref047","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1093\/bioinformatics\/btw413","article-title":"MorphoLibJ: integrated library and plugins for mathematical morphology with ImageJ","volume":"32","author":"D 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