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However, the understanding of phages that can specifically infect encapsulated bacteria\u2014particularly myophages\u2014remains limited, especially regarding their structures with multi-states, and infection and contraction mechanisms, such as tail fiber conformational changes and what triggers tail contraction. In this study, we resolved the intact structures of phi92, which possesses a broad host range encompassing both encapsulated and non-encapsulated strains of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strains and diverse\n                    <jats:italic>Salmonella<\/jats:italic>\n                    strains, in both its extended and contracted states by cryo-electron microscopy (cryo-EM). We identified and built atomic models for most components in the head, connector, tail, and baseplate. Notably, we inferred that one of the three fibers corresponds to fiber I (gp143) and identified another as fiber III (gp147). We propose that fiber I specifically degrades host capsular polysaccharides, while fiber III mediates stable adsorption to the host cell membrane. Phi92 achieves broad host adaptability through its multiple fibers, thereby conferring a significant competitive advantage when infecting bacteria with distinct types. Comparison of the two states reveals that significant conformational rearrangements of fiber III and baseplate periphery play a pivotal role in triggering sheath contraction. This study elucidates the trigger mechanism of the contractile nanomachine in phi92-like myophages with a baseplate architecture, providing a crucial structural foundation for developing myophage-based therapies against encapsulated, drug-resistant bacteria.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014494","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:25:28Z","timestamp":1786123528000},"page":"e1014494","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intact architectures of myophage phi92 in extended and contracted states"],"prefix":"10.1371","volume":"22","author":[{"given":"Yuan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuning","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yewei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binning","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6247-5464","authenticated-orcid":true,"given":"Hongrong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"ppat.1014494.ref001","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1146\/annurev.micro.62.081307.162944","article-title":"Capsules 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