{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:28:21Z","timestamp":1789658901527,"version":"4.0.1"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Fujian Research and Training Grants for Young and Middle-aged Leaders in Healthcare","award":["No.2023WJWZQN01"],"award-info":[{"award-number":["No.2023WJWZQN01"]}]},{"name":"the National Natural Science Foundation of China","award":["No.82472256"],"award-info":[{"award-number":["No.82472256"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"the Foundation of Fujian Provincial Finance Department","award":["No.22SCZZX016"],"award-info":[{"award-number":["No.22SCZZX016"]}],"id":[{"id":"https:\/\/ror.org\/01hrpa353","id-type":"ROR","asserted-by":"crossref"}]},{"name":"the Natural Science Foundation of Fujian Province","award":["No.2023J02015"],"award-info":[{"award-number":["No.2023J02015"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Epstein-Barr virus (EBV) is an oncogenic DNA virus that is etiologically associated with multiple malignancies, including nasopharyngeal carcinoma, gastric carcinoma, and lymphomas. Although EBV encodes numerous viral\u00a0microRNAs\u00a0(miRNAs), the pathogenic functions of individual\u00a0miRNAs\u00a0remain incompletely characterized. In this study, we identify EBV-encoded miR-BART17-5p as a tumor-suppressive miRNA that directly targets ceramide kinase (CERK). CERK expression is significantly downregulated in EBV-associated gastric cancer (EBVaGC) tissues and EBV-positive AGS-EBV cells and inversely correlates with miR-BART17-5p levels. Mechanistic analyses further demonstrate that miR-BART17-5p directly targets the 3\u2032 untranslated region (3\u2032UTR) of\n                    <jats:italic>CERK<\/jats:italic>\n                    , thereby regulating its expression. Overexpression of miR-BART17-5p mimics or knockdown of\n                    <jats:italic>CERK<\/jats:italic>\n                    in AGS cells significantly suppresses tumor -associated phenotypes, including cell proliferation, migration, and invasion. Conversely, inhibition of miR-BART17-5p in AGS-EBV cells rescues these phenotypes. Similar results were obtained in SNU719 cells. Mechanistically, miR-BART17-5p inhibits the PI3K\/AKT signaling by targeting\n                    <jats:italic>CERK<\/jats:italic>\n                    . Restoration of CERK expression reactivates PI3K\/AKT signaling and reverses the inhibitory effects on cell proliferation, migration, and invasion in AGS cells. To evaluate the therapeutic potential of miR-BART17-5p, systemic administration of engineered cholesterol-conjugated miR-BART17-5p agomirs significantly suppresses tumor growth in xenograft models. Collectively, our findings reveal a previously unrecognized EBV-host interaction whereby miR-BART17-5p constrains cancer progression through modulation of CERK\/PI3K\/AKT signaling activation, highlighting its potential as a preclinical therapeutic strategy for cancer.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014519","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:46:31Z","timestamp":1789494391000},"page":"e1014519","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epstein-Barr virus-encoded microRNA-BART17-5p suppresses cell proliferation and metastasis by targeting CERK"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0593-8519","authenticated-orcid":true,"given":"Peilin","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]},{"name":"the First Affiliated Hospital of Fujian Medical University","place":["Fuzhou, China"],"department":["Department of paediatrics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiazhen","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]},{"name":"School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"],"department":["Fujian Key Laboratory of Tumor Microbiology, Department of Medical Microbiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]},{"name":"School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"],"department":["Fujian Key Laboratory of Tumor Microbiology, Department of Medical Microbiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Xin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]},{"name":"School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"],"department":["Fujian Key Laboratory of Tumor Microbiology, Department of Medical Microbiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1236-2386","authenticated-orcid":true,"given":"Wannan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"]},{"name":"School of Basic Medical Sciences, Fujian Medical University","place":["Fuzhou, China"],"department":["Fujian Key Laboratory of Tumor Microbiology, Department of Medical Microbiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"issue":"7","key":"ppat.1014519.ref001","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1056\/NEJM200008173430707","article-title":"Epstein-Barr virus infection","volume":"343","author":"JI Cohen","year":"2000","journal-title":"N Engl J 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