{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:09:20Z","timestamp":1789063760210,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.ppat.1014569","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000}}],"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS-CSLPDCP-202401"],"award-info":[{"award-number":["CAAS-CSLPDCP-202401"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["No. Y2026YC66"],"award-info":[{"award-number":["No. Y2026YC66"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No:32573329"],"award-info":[{"award-number":["No:32573329"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a bat-originated alphacoronavirus that causes devastating enteric disease in neonatal piglets and possesses significant potential for cross-species transmission. While the early stages of the coronavirus life cycle have been extensively characterized, the host factors indispensable for virion assembly and subsequent export remain largely enigmatic. Here, by performing a genome-wide CRISPR-Cas9 knockout screen using a recombinant icSADS-CoV-GFP reporter virus, we identified the small GTPase Rab10 as a critical host dependency factor for SADS-CoV infection. Viral life cycle analysis revealed that Rab10 is not required for viral attachment, entry, or initial genome replication, but is essential for the virion transport and non-lytic egress. Rab10 deficiency markedly reduced the extracellular release of viral RNA, viral proteins, and infectious progeny, as well as the secretion of SADS-CoV virus-like particles. Confocal imaging showed that Rab10 and viral protein-positive intracellular structures were associated with LMAN1, TGN46, and LAMP1 positive compartments. These findings support a model in which Rab10 coordinates a virus-containing vesicles trafficking pathway associated with ERGIC-TGN-lysosome compartments. Mechanistically, Rab10 facilitates the loading of the viral envelope (E) protein into transport vesicles derived from the ERGIC. Rab10 associates with the SADS-CoV E protein, and mapping analyses implicated the C-terminal PDZ-binding motif, particularly residue V75, in efficient Rab10 association and viral release. Collectively, our findings identify Rab10 as a host regulator of SADS-CoV non-lytic egress and highlight the E-Rab10 interaction and the vesicular trafficking\u00a0machinery\u00a0as a potential target for developing antiviral strategies.<\/jats:p>","DOI":"10.1371\/journal.ppat.1014569","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:50:02Z","timestamp":1788285002000},"page":"e1014569","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rab10 coordinates SADS-CoV non-lytic egress through the ERGIC-TGN-lysosome trafficking pathway"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0175-4339","authenticated-orcid":true,"given":"Liaoyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Xue","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Sun","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dakai","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Zeng","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengting","family":"Gu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Shi","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfei","family":"Chen","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Ji","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1386-775X","authenticated-orcid":true,"given":"Da","family":"Shi","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2898-8308","authenticated-orcid":true,"given":"Li","family":"Feng","sequence":"additional","affiliation":[{"name":"Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute","place":["Harbin, China"],"department":["State Key Laboratory of Animal Disease Control and Prevention"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"issue":"7700","key":"ppat.1014569.ref001","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/s41586-018-0010-9","article-title":"Fatal swine acute diarrhoea syndrome caused by an HKU2-related coronavirus of bat origin","volume":"556","author":"P Zhou","year":"2018","journal-title":"Nature"},{"issue":"9","key":"ppat.1014569.ref002","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.01197-24","article-title":"Diversity and spillover risk of swine acute diarrhea syndrome and related coronaviruses in China and Southeast Asia","volume":"16","author":"A Latinne","year":"2025","journal-title":"mBio"},{"issue":"2","key":"ppat.1014569.ref003","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/tbed.13008","article-title":"Retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern China","volume":"66","author":"L Zhou","year":"2019","journal-title":"Transbound Emerg Dis"},{"issue":"1","key":"ppat.1014569.ref004","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13567-024-01445-0","article-title":"Molecular characterization of swine acute diarrhea syndrome coronavirus detected in Vietnamese pigs","volume":"56","author":"NP Le","year":"2025","journal-title":"Vet Res"},{"issue":"1","key":"ppat.1014569.ref005","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s12985-025-02834-3","article-title":"Development of an indirect enzyme-linked immunosorbent assay based on nucleocapsid protein for the detection of swine acute diarrhea syndrome coronavirus antibody","volume":"22","author":"Y-J Jang","year":"2025","journal-title":"Virol J"},{"issue":"24","key":"ppat.1014569.ref006","article-title":"Broad Cross-Species Infection of Cultured Cells by Bat HKU2-Related Swine Acute Diarrhea Syndrome Coronavirus and Identification of Its Replication in Murine Dendritic Cells In Vivo Highlight Its Potential for Diverse Interspecies Transmission","volume":"93","author":"Y-L Yang","year":"2019","journal-title":"J Virol"},{"issue":"5","key":"ppat.1014569.ref007","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02371-21","article-title":"Betacoronavirus Assembly: Clues and Perspectives for Elucidating SARS-CoV-2 Particle Formation and Egress","volume":"12","author":"D Bracquemond","year":"2021","journal-title":"mBio"},{"issue":"6","key":"ppat.1014569.ref008","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1111\/mmi.14907","article-title":"The life cycle and enigmatic egress of coronaviruses","volume":"117","author":"K Prydz","year":"2022","journal-title":"Mol Microbiol"},{"issue":"6","key":"ppat.1014569.ref009","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1013295","article-title":"SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion","volume":"21","author":"J Zhang","year":"2025","journal-title":"PLoS Pathog"},{"issue":"8","key":"ppat.1014569.ref010","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.3390\/cells10082047","article-title":"Secretory Vesicles Are the Principal Means of SARS-CoV-2 Egress","volume":"10","author":"S Eymieux","year":"2021","journal-title":"Cells"},{"issue":"23","key":"ppat.1014569.ref011","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1016\/j.devcel.2021.10.006","article-title":"ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress","volume":"56","author":"D Chen","year":"2021","journal-title":"Dev Cell"},{"issue":"12","key":"ppat.1014569.ref012","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01338-23","article-title":"The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells","volume":"97","author":"Z Wang","year":"2023","journal-title":"J Virol"},{"issue":"6","key":"ppat.1014569.ref013","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1016\/j.cell.2020.10.039","article-title":"\u03b2-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway","volume":"183","author":"S Ghosh","year":"2020","journal-title":"Cell"},{"issue":"5","key":"ppat.1014569.ref014","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/nrg3899","article-title":"High-throughput functional genomics using CRISPR-Cas9","volume":"16","author":"O Shalem","year":"2015","journal-title":"Nat Rev Genet"},{"issue":"11","key":"ppat.1014569.ref015","article-title":"A review of virus host factor discovery using CRISPR screening","volume":"15","author":"WR See","year":"2024","journal-title":"mBio"},{"issue":"18","key":"ppat.1014569.ref016","article-title":"Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection","volume":"119","author":"LV Tse","year":"2022","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"ppat.1014569.ref017","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009599","article-title":"TMEM41B is a host factor required for the replication of diverse coronaviruses including SARS-CoV-2","volume":"17","author":"JD Trimarco","year":"2021","journal-title":"PLoS Pathog"},{"issue":"5","key":"ppat.1014569.ref018","article-title":"Genome-scale CRISPR screen identifies TMEM198 driving double membrane vesicle formation in swine alphacoronavirus and murine betacoronavirus infected cells","volume":"21","author":"L Shi","year":"2025","journal-title":"PLoS Pathog"},{"issue":"6","key":"ppat.1014569.ref019","article-title":"YIPF5 is an essential host factor for porcine epidemic diarrhea virus double-membrane vesicle formation","volume":"99","author":"L Guo","year":"2025","journal-title":"J Virol"},{"issue":"5","key":"ppat.1014569.ref020","article-title":"Identification of ZDHHC17 as a Potential Drug Target for Swine Acute Diarrhea Syndrome Coronavirus Infection","volume":"12","author":"Y Luo","year":"2021","journal-title":"mBio"},{"issue":"4","key":"ppat.1014569.ref021","article-title":"RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking","volume":"224","author":"S Liu","year":"2025","journal-title":"J Cell Biol"},{"key":"ppat.1014569.ref022","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1038\/ncomms3005","article-title":"Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development","volume":"4","author":"Y Liu","year":"2013","journal-title":"Nat Commun"},{"issue":"12","key":"ppat.1014569.ref023","article-title":"Genome-scale CRISPR screen identifies TMEM41B as a multi-function host factor required for coronavirus replication","volume":"17","author":"L Sun","year":"2021","journal-title":"PLoS Pathog"},{"issue":"6","key":"ppat.1014569.ref024","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/boc.202000146","article-title":"Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro","volume":"113","author":"LA Caldas","year":"2021","journal-title":"Biol Cell"},{"issue":"1","key":"ppat.1014569.ref025","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abl4895","article-title":"SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi\/ERGIC and lysosome-mediated egress","volume":"8","author":"KM Scherer","year":"2022","journal-title":"Sci Adv"},{"issue":"1","key":"ppat.1014569.ref026","doi-asserted-by":"crossref","first-page":"4629","DOI":"10.1038\/s41467-021-24887-y","article-title":"Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress","volume":"12","author":"L Mendon\u00e7a","year":"2021","journal-title":"Nat Commun"},{"key":"ppat.1014569.ref027","doi-asserted-by":"crossref","first-page":"110285","DOI":"10.1016\/j.virol.2024.110285","article-title":"Strengths and limitations of SARS-CoV-2 virus-like particle systems","volume":"601","author":"R Sultana","year":"2025","journal-title":"Virology"},{"key":"ppat.1014569.ref028","doi-asserted-by":"crossref","first-page":"100111","DOI":"10.1074\/jbc.RA120.016175","article-title":"The SARS-CoV-2 envelope and membrane proteins modulate maturation and retention of the spike protein, allowing assembly of virus-like particles","volume":"296","author":"B Boson","year":"2021","journal-title":"J Biol Chem"},{"issue":"5","key":"ppat.1014569.ref029","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf133","article-title":"Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles for mRNA delivery","volume":"53","author":"Q Yang","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"ppat.1014569.ref030","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.ijbiomac.2022.01.108","article-title":"Review: A systematic review of virus-like particles of coronavirus: Assembly, generation, chimerism and their application in basic research and in the clinic","volume":"200","author":"W Lu","year":"2022","journal-title":"Int J Biol Macromol"},{"key":"ppat.1014569.ref031","doi-asserted-by":"crossref","DOI":"10.1111\/tra.70001","article-title":"Disease-associated factors at the endoplasmic reticulum-golgi interface","volume":"26","author":"M Maeda","year":"2025","journal-title":"Traffic"},{"key":"ppat.1014569.ref032","doi-asserted-by":"crossref","first-page":"884360","DOI":"10.3389\/fcell.2022.884360","article-title":"The Golgi Apparatus and its Next-Door Neighbors","volume":"10","author":"A Nakano","year":"2022","journal-title":"Front Cell Dev Biol"},{"issue":"1","key":"ppat.1014569.ref033","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/s41421-022-00510-2","article-title":"Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2","volume":"9","author":"B Xia","year":"2023","journal-title":"Cell Discov"},{"issue":"9","key":"ppat.1014569.ref034","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.tcb.2023.12.006","article-title":"Membrane remodeling and trafficking piloted by SARS-CoV-2","volume":"34","author":"MC Sergio","year":"2024","journal-title":"Trends Cell Biol"},{"issue":"1","key":"ppat.1014569.ref035","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1152\/physrev.00059.2009","article-title":"Role of Rab GTPases in membrane traffic and cell physiology","volume":"91","author":"AH Hutagalung","year":"2011","journal-title":"Physiol Rev"},{"issue":"23","key":"ppat.1014569.ref036","article-title":"Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-, Cholesterol-, and pH-Dependent Manner","volume":"91","author":"Z Li","year":"2017","journal-title":"J Virol"},{"issue":"1","key":"ppat.1014569.ref037","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13567-020-0739-7","article-title":"PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-\/lysosome pathway","volume":"51","author":"XN Wei","year":"2020","journal-title":"Vet Res"},{"issue":"24","key":"ppat.1014569.ref038","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01345-21","article-title":"Porcine Deltacoronavirus Enters Porcine IPI-2I Intestinal Epithelial Cells via Macropinocytosis and Clathrin-Mediated Endocytosis Dependent on pH and Dynamin","volume":"95","author":"P Fang","year":"2021","journal-title":"J Virol"},{"issue":"1","key":"ppat.1014569.ref039","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13567-024-01328-4","article-title":"Pseudorabies virus hijacks the Rab6 protein to promote viral assembly and egress","volume":"55","author":"D-G Liang","year":"2024","journal-title":"Vet Res"},{"issue":"4","key":"ppat.1014569.ref040","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02618-24","article-title":"Classical swine fever virus recruits ALIX and ESCRT-III to facilitate viral budding","volume":"16","author":"J Chen","year":"2025","journal-title":"mBio"},{"issue":"8","key":"ppat.1014569.ref041","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1012504","article-title":"Rab27a GTPase and its effector Myosin Va are host factors required for efficient Oropouche virus cell egress","volume":"20","author":"JO Concha","year":"2024","journal-title":"PLoS Pathog"},{"issue":"1","key":"ppat.1014569.ref042","doi-asserted-by":"crossref","first-page":"6271","DOI":"10.1038\/s41467-025-61587-3","article-title":"Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane","volume":"16","author":"T Chen","year":"2025","journal-title":"Nat Commun"},{"issue":"9","key":"ppat.1014569.ref043","article-title":"Role of the early secretory pathway in SARS-CoV-2 infection","volume":"219","author":"D Sicari","year":"2020","journal-title":"J Cell Biol"},{"issue":"8058","key":"ppat.1014569.ref044","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/s41586-025-08773-x","article-title":"A coronavirus assembly inhibitor that targets the viral membrane protein","volume":"640","author":"M Laporte","year":"2025","journal-title":"Nature"},{"issue":"1","key":"ppat.1014569.ref045","doi-asserted-by":"crossref","first-page":"6326","DOI":"10.1038\/s41467-025-61431-8","article-title":"Host protein ARF1 is a proviral factor for SARS-CoV-2 and a candidate broad-spectrum therapeutic target","volume":"16","author":"C Zhang","year":"2025","journal-title":"Nat Commun"},{"issue":"10","key":"ppat.1014569.ref046","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00426-23","article-title":"Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells","volume":"97","author":"K Miura","year":"2023","journal-title":"J Virol"},{"issue":"14","key":"ppat.1014569.ref047","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adl5012","article-title":"ER-export and ARFRP1\/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication","volume":"10","author":"GJ Pearson","year":"2024","journal-title":"Sci Adv"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1014569","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1014569","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:47:14Z","timestamp":1789062434000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1014569"}},"subtitle":[],"editor":[{"given":"Chee Wah","family":"Tan","sequence":"first","affiliation":[{"name":"National University of Singapore","place":["SINGAPORE"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":47,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1014569","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}