{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:04:20Z","timestamp":1789495460231,"version":"build-2803163510"},"reference-count":84,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    In Arabidopsis, BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)\n                    <jats:italic>-<\/jats:italic>\n                    INTERACTING RECEPTOR-LIKE KINASE 1 (BIR1) is a negative regulator of plant immunity and cell death.\n                    <jats:italic>BIR1<\/jats:italic>\n                    was earlier described as a target of epigenetic and post-transcriptional degradation. During virus infections, degradome analysis of\n                    <jats:italic>BIR1<\/jats:italic>\n                    transcripts mapped predominant mRNA cleavage sites at the 5\u2019-untranslated leader region (site A) and the protein-coding sequence (sites B and C). Here, we identified another virus-associated cleavage site (D) within the\n                    <jats:italic>BIR1<\/jats:italic>\n                    coding region and investigated the contribution of site-directed mRNA cleavage to\n                    <jats:italic>BIR1<\/jats:italic>\n                    regulation. Mutations at B, C, and D sites enhanced mRNA stability by impairing transcript cleavage, resulting in increased\n                    <jats:italic>BIR1<\/jats:italic>\n                    mRNA and protein accumulation. This regulation is disrupted in RNA silencing mutants, supporting a model of\n                    <jats:italic>cis<\/jats:italic>\n                    -directed small interfering RNA (siRNA)-mediated degradation. We next demonstrate that virus infection reduces\n                    <jats:italic>BIR1<\/jats:italic>\n                    translation in Arabidopsis. Furthermore, our data reveal a repressive role for the 5\u2019-leader in regulating\n                    <jats:italic>BIR1<\/jats:italic>\n                    translation, potentially mediated by upstream open reading frames (uORFs) and a virus-responsive long non-coding RNA (lncRNA) derived from the natural antisense\n                    <jats:italic>At4g39838<\/jats:italic>\n                    locus. Together, these findings reveal a multilayered regulatory mechanism that integrates sRNA-mediated cleavage with translational control, with broader implications for the fine-tuning of stress-responsive gene expression during infection.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014571","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:26:52Z","timestamp":1789493212000},"page":"e1014571","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual regulation of the receptor-like kinase BIR1 involves site-directed transcript cleavage and 5\u2019-leader-mediated translational control"],"prefix":"10.1371","volume":"22","author":[{"given":"Irene","family":"Guzm\u00e1n-Benito","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas Margarita Salas. Consejo Superior de Investigaciones Cient\u00edficas","place":["Ramiro de Maeztu, Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Robinson","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas Margarita Salas. Consejo Superior de Investigaciones Cient\u00edficas","place":["Ramiro de Maeztu, Madrid, Spain"]},{"name":"Doctorado en Biotecnolog\u00eda y Recursos Gen\u00e9ticos de Plantas y Microorganismos Asociados, Universidad Polit\u00e9cnica de Madrid","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Donaire","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas Margarita Salas. Consejo Superior de Investigaciones Cient\u00edficas","place":["Ramiro de Maeztu, Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Fern\u00e1ndez-Calvino","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas Margarita Salas. Consejo Superior de Investigaciones Cient\u00edficas","place":["Ramiro de Maeztu, Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Franco-Zorrilla","sequence":"additional","affiliation":[{"name":"Consejo Superior de Investigaciones Cient\u00edficas","place":["Darwin, Madrid, Spain"],"department":["Department of Plant Molecular Genetics. Centro Nacional de Biotecnolog\u00eda"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixia","family":"Niu","sequence":"additional","affiliation":[{"name":"RNA Institute, Institute for Advanced Studies (IAS), Wuhan University","place":["Wuhan, Hubei, China"],"department":["State Key Laboratory of Hybrid Rice, Hubei Provincial Research Center for Basic Biological Sciences, Hubei Hongshan Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyong","family":"Xu","sequence":"additional","affiliation":[{"name":"RNA Institute, Institute for Advanced Studies (IAS), Wuhan University","place":["Wuhan, Hubei, China"],"department":["State Key Laboratory of Hybrid Rice, Hubei Provincial Research Center for Basic Biological Sciences, Hubei Hongshan Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catharina","family":"Merchante","sequence":"additional","affiliation":[{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea La Mayora (IHSM-UMA-CSIC)","place":["Boulevard Louis Pasteur, M\u00e1laga, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3844-4582","authenticated-orcid":true,"given":"C\u00e9sar","family":"Llave","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas Margarita Salas. 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