{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:01:27Z","timestamp":1790704887827,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1014619","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000}}],"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2024YFE0198900"],"award-info":[{"award-number":["2024YFE0198900"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2024YFC2310800"],"award-info":[{"award-number":["2024YFC2310800"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (\n                    <jats:italic>Kp<\/jats:italic>\n                    ) is an opportunistic Gram-negative pathogen responsible for severe community- and healthcare-associated infections. Resistance to complement-mediated killing is an important determinant of\n                    <jats:italic>Kp<\/jats:italic>\n                    survival in human serum, yet mechanisms beyond capsular polysaccharide-mediated protection remain incompletely understood. Here, using comparative proteomic approaches, we identified a previously uncharacterized protein, GM000287, as a conserved determinant of serum resistance and virulence in\n                    <jats:italic>Kp<\/jats:italic>\n                    . Human serum exposure induced expression of the bacterial type II secretion system (T2SS), and deletion of the essential T2SS components GspD\/GspE increased susceptibility to human serum killing. Importantly, disruption of GspD\/GspE attenuated virulence in a murine pneumonia model. Comparative secretome analysis identified GM000287 as a T2SS-associated envelope protein that contributes to serum survival. Loss of GM000287 increased C3b production and factor B deposition on the bacterial surface during human serum exposure, consistent with enhanced alternative pathway amplification. In a murine pneumonia model, deletion of GM000287 reduced bacterial persistence and dissemination and attenuated virulence. Given the minimal bactericidal activity of mouse serum against\n                    <jats:italic>Kp<\/jats:italic>\n                    , indicating an additional role for GM000287 during infection. Importantly, GM000287 was highly conserved among\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    species and broadly distributed among members of the\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    family, and its contribution to serum resistance was validated in genetically diverse\n                    <jats:italic>Kp<\/jats:italic>\n                    clinical isolates. Together, these findings identify GM000287 as a conserved determinant of human serum resistance and\n                    <jats:italic>in vivo<\/jats:italic>\n                    virulence and reveal distinct contributions of GM000287 to\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    fitness across different host-associated environments.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014619","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:48:57Z","timestamp":1790272137000},"page":"e1014619","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["GM000287 is a conserved determinant of serum resistance and virulence in Klebsiella pneumoniae"],"prefix":"10.1371","volume":"22","author":[{"given":"Xiaomin","family":"Lin","sequence":"first","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Public Health and Emergency Management, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjun","family":"Jia","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Public Health and Emergency Management, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zilin","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Mo","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Public Health and Emergency Management, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxin","family":"Pan","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Public Health and Emergency Management, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaining","family":"Zhong","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Public Health and Emergency Management, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Shen","sequence":"additional","affiliation":[{"name":"Zhejiang University School of Medicine","place":["Hangzhou, China"],"department":["State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectio..."]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanjiang","family":"Ji","sequence":"additional","affiliation":[{"name":"Shanghai Tech University","place":["Shanghai, China"],"department":["School of Physical Science and Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shenzhen University Medical School, Shenzhen University","place":["Shenzhen, China"],"department":["Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Pathogen Biology"]},{"name":"Shenzhen People's Hospital (The First Affiliated Hospital, Southern University of Science and Technology, The Second Clinical Medical College, Jinan University)","place":["Shenzhen, China"],"department":["Shenzhen Key Laboratory of Respiratory Diseases, Shenzhen Clinical Research Center for Respiratory Disease, Shenzhen Institute of Respiratory Diseases"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Yang","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Zhejiang University School of Medicine","place":["Hangzhou, China"],"department":["State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectio..."]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7116-0501","authenticated-orcid":true,"given":"Chunfu","family":"Yang","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["School of Public Health and Emergency Management, School of Medicine"]},{"name":"Southern University of Science and Technology","place":["Shenzhen, China"],"department":["SUSTech Homeostatic Medicine Institute, School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"issue":"10325","key":"ppat.1014619.ref001","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Antimicrobial Resistance 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