{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:53:33Z","timestamp":1791226413345,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1014626","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000}}],"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["A1077784"],"award-info":[{"award-number":["A1077784"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI153155"],"award-info":[{"award-number":["AI153155"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Rickettsia parkeri,<\/jats:italic>\n                    an emerging tick-borne pathogen, is transmitted by\n                    <jats:italic>Amblyomma maculatum.<\/jats:italic>\n                    Initial bacterial inoculation occurs in the skin via infected tick bite. During blood acquisition, tick salivary factors manipulate host hemostatic and immune responses. Examination of rickettsial pathogenesis and the host response to infection in murine models primarily utilize needle inoculation. As a result, biological variables including transmission dynamics and the influence of vector saliva on the host response at the infection site are undefined. To test the hypothesis that a distinct host cutaneous immune response will be elicited based on the infection status of the tick, a single tick feeding model was developed to assess the transmission and dissemination kinetics of\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    from the early stage of tick attachment (1 hour) to late feeding stage (144 hours). Furthermore, changes in the cutaneous immune response were characterized through immunophenotyping, cytokine and chemokine profiling, and histopathological analysis. The data show that transmission of\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    from the infected tick to the host occurs within 1 hour-post tick attachment (hpa), with rapid dissemination to lymph nodes and internal tissues. The quantifiable bacterial load in the mouse skin delivered by an infected tick was 9,500 bacteria at 3 hours, increasing to 15,000 and 22,000 rickettsiae at 48 and 144 hours, respectively. Independent of tick infection status, the degree of time-dependent inflammation was similar at 3 and 48 hpa. However, in\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    -infected skin at 144 hpa, macrophages were the dominant cell type, with enhanced levels of IFN-\u03b3 and TNF-\u03b1 while IL-10 production was reduced. The data suggest delayed pro-inflammatory activation in response to rickettsial infection when delivered by ticks. This study yields valuable insights into the early events of\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    transmission and the host response, providing the basis for the development of preventive strategies against tick-borne rickettsial disease.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014626","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:51:18Z","timestamp":1790617878000},"page":"e1014626","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spotted fever group Rickettsia infection kinetics and cutaneous host response during single tick feeding events"],"prefix":"10.1371","volume":"22","author":[{"given":"Chanakan","family":"Suwanbongkot","sequence":"first","affiliation":[{"name":"Frederick P. Whiddon College of Medicine, University of South Alabama","place":["Mobile, Alabama, United States of America"],"department":["Department of Microbiology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingeborg M.","family":"Langohr","sequence":"additional","affiliation":[{"name":"Global Discovery Pathology & Multimodal Imaging, Translation Models Research Platform, Sanofi","place":["Cambridge, Massachusetts, United States of America"]},{"name":"Louisiana State University","place":["Baton Rouge, Louisiana, United States of America"],"department":["Department of Pathobiological Sciences, School of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Killian Z.","family":"Brewer","sequence":"additional","affiliation":[{"name":"Frederick P. Whiddon College of Medicine, University of South Alabama","place":["Mobile, Alabama, United States of America"],"department":["Department of Microbiology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Barrington","sequence":"additional","affiliation":[{"name":"Frederick P. Whiddon College of Medicine, University of South Alabama","place":["Mobile, Alabama, United States of America"],"department":["Department of Microbiology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8537-6100","authenticated-orcid":true,"given":"Kevin R.","family":"Macaluso","sequence":"additional","affiliation":[{"name":"Frederick P. Whiddon College of Medicine, University of South Alabama","place":["Mobile, Alabama, United States of America"],"department":["Department of Microbiology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"issue":"6","key":"ppat.1014626.ref001","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1086\/381894","article-title":"Rickettsia parkeri: A newly recognized cause of spotted fever rickettsiosis in the United States","volume":"38","author":"CD Paddock","year":"2004","journal-title":"Clin Infect Dis"},{"issue":"10","key":"ppat.1014626.ref002","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.3201\/eid3010.240821","article-title":"Establishment of Amblyomma maculatum ticks and Rickettsia parkeri the Northeastern United States","volume":"30","author":"G Molaei","year":"2024","journal-title":"Emerg Infect 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rickettsii are differentially modulated by either temperature upshift or blood-feeding in tick midgut and salivary glands","volume":"9","author":"MFBM Galletti","year":"2016","journal-title":"Parasit Vectors"},{"issue":"3","key":"ppat.1014626.ref007","doi-asserted-by":"crossref","first-page":"268","DOI":"10.4269\/ajtmh.2004.71.3.0700268","article-title":"Short report: Duration of tick attachment required for transmission of powassan virus by deer ticks","volume":"71","author":"GD Ebel","year":"2004","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"ppat.1014626.ref008","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.ttbdis.2018.01.002","article-title":"Pathogen transmission in relation to duration of attachment by Ixodes scapularis ticks","volume":"9","author":"L Eisen","year":"2018","journal-title":"Ticks Tick Borne Dis"},{"issue":"5","key":"ppat.1014626.ref009","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1086\/318818","article-title":"Effect of tick removal on transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis nymphs","volume":"183","author":"F des Vignes","year":"2001","journal-title":"J Infect Dis"},{"issue":"5","key":"ppat.1014626.ref010","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1086\/517829","article-title":"Duration of tick attachment required for transmission of granulocytic ehrlichiosis","volume":"177","author":"P Katavolos","year":"1998","journal-title":"J Infect Dis"},{"issue":"3","key":"ppat.1014626.ref011","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1128\/jcm.25.3.557-558.1987","article-title":"Duration of tick attachment and Borrelia burgdorferi transmission","volume":"25","author":"J Piesman","year":"1987","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ppat.1014626.ref012","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1093\/infdis\/163.4.895","article-title":"Duration of adult female Ixodes dammini attachment and transmission 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Herrick","year":"2016","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"ppat.1014626.ref016","doi-asserted-by":"crossref","first-page":"910","DOI":"10.3390\/microorganisms8060910","article-title":"Interrupted blood feeding in ticks: Causes and consequences","volume":"8","author":"D Tahir","year":"2020","journal-title":"Microorganisms"},{"key":"ppat.1014626.ref017","volume-title":"Biology of ticks volume 2","author":"DE Sonenshine","year":"2014"},{"key":"ppat.1014626.ref018","doi-asserted-by":"crossref","first-page":"809052","DOI":"10.3389\/fcimb.2022.809052","article-title":"Host immune responses to salivary components - A critical facet of tick-host interactions","volume":"12","author":"A Ali","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"ppat.1014626.ref019","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3389\/fcimb.2017.00281","article-title":"The essential role of tick salivary glands and saliva in tick feeding and pathogen 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Med"},{"issue":"1","key":"ppat.1014626.ref032","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4269\/ajtmh.2001.65.52","article-title":"Rickettsial infection in murine models activates an early anti-rickettsial effect mediated by NK cells and associated with production of gamma interferon","volume":"65","author":"AN Billings","year":"2001","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"ppat.1014626.ref033","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1128\/IAI.69.3.1841-1846.2001","article-title":"Critical role of cytotoxic T lymphocytes in immune clearance of rickettsial infection","volume":"69","author":"DH Walker","year":"2001","journal-title":"Infect Immun"},{"issue":"11","key":"ppat.1014626.ref034","doi-asserted-by":"crossref","first-page":"5314","DOI":"10.4049\/jimmunol.158.11.5314","article-title":"Role of T lymphocyte subsets in immunity to spotted fever group Rickettsiae","volume":"158","author":"H Feng","year":"1997","journal-title":"J Immunol"},{"issue":"3","key":"ppat.1014626.ref035","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s00281-015-0480-x","article-title":"The role of CD8 T lymphocytes in rickettsial infections","volume":"37","author":"DH Walker","year":"2015","journal-title":"Semin Immunopathol"},{"issue":"6","key":"ppat.1014626.ref036","doi-asserted-by":"crossref","first-page":"102025","DOI":"10.1016\/j.ttbdis.2022.102025","article-title":"Tick species infesting humans in the United States","volume":"13","author":"L Eisen","year":"2022","journal-title":"Ticks Tick Borne Dis"},{"issue":"2","key":"ppat.1014626.ref037","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3390\/environments4020037","article-title":"Do tick attachment times vary between different tick-pathogen systems?","volume":"4","author":"S Richards","year":"2017","journal-title":"Environments"},{"issue":"2","key":"ppat.1014626.ref038","first-page":"585","article-title":"Minimal duration of tick attachment sufficient for transmission of infectious Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) by Its Primary Vector Dermacentor variabilis (Acari: Ixodidae): Duration of rickettsial reactivation in the vector revisited","volume":"57","author":"ML Levin","year":"2020","journal-title":"J Med Entomol"},{"issue":"17","key":"ppat.1014626.ref039","doi-asserted-by":"crossref","first-page":"496","DOI":"10.15585\/mmwr.mm6717e1","article-title":"Vital Signs: Trends in reported vectorborne disease cases - United States and Territories, 2004-2016","volume":"67","author":"R Rosenberg","year":"2018","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"4","key":"ppat.1014626.ref040","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1093\/infdis\/jiae512","article-title":"Rickettsia rickettsii subsp californica subsp nov, the Etiologic Agent of Pacific Coast Tick Fever","volume":"231","author":"CD Paddock","year":"2025","journal-title":"J Infect 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One"},{"issue":"2","key":"ppat.1014626.ref044","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1111\/brv.12384","article-title":"Tick attachment cement - reviewing the mysteries of a biological skin plug system","volume":"93","author":"J Suppan","year":"2018","journal-title":"Biol Rev Camb Philos Soc"},{"issue":"5","key":"ppat.1014626.ref045","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1093\/jmedent\/32.5.578","article-title":"Bacteriocidal qualities of ixodid tick (Acarina: Ixodidae) salivary cement plugs and their changes under the influence of a viral tick-borne pathogen","volume":"32","author":"AN Alekseev","year":"1995","journal-title":"J Med Entomol"},{"issue":"1","key":"ppat.1014626.ref046","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182000071845","article-title":"Observations on the movement of fluids in the vicinity of the mouthparts of naturally feeding Dermacentor andersoni Stiles","volume":"57","author":"JD Gregson","year":"1967","journal-title":"Parasitology"},{"issue":"2","key":"ppat.1014626.ref047","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/jmedent\/45.2.267","article-title":"Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction","volume":"45","author":"AS Zanettii","year":"2008","journal-title":"J Med Entomol"},{"issue":"6","key":"ppat.1014626.ref048","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.ttbdis.2014.05.009","article-title":"Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs","volume":"5","author":"N Milhano","year":"2014","journal-title":"Ticks Tick Borne Dis"},{"issue":"6","key":"ppat.1014626.ref049","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007054","article-title":"A biosafety level-2 dose-dependent lethal mouse model of spotted fever rickettsiosis: Rickettsia parkeri Atlantic Rainforest 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Physiol"},{"issue":"60","key":"ppat.1014626.ref053","doi-asserted-by":"crossref","DOI":"10.3791\/3894-v","article-title":"Saliva, salivary gland, and hemolymph collection from Ixodes scapularis ticks","author":"TG Patton","year":"2012","journal-title":"J Vis Exp"},{"issue":"11","key":"ppat.1014626.ref054","doi-asserted-by":"crossref","DOI":"10.1128\/iai.00282-23","article-title":"Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite","volume":"91","author":"X Tang","year":"2023","journal-title":"Infect Immun"},{"issue":"4","key":"ppat.1014626.ref055","doi-asserted-by":"crossref","DOI":"10.1128\/iai.00065-25","article-title":"Peromyscus leucopus, Mus musculus, and humans have distinct transcriptomic responses to larval Ixodes scapularis bites","volume":"93","author":"JS Bourgeois","year":"2025","journal-title":"Infect 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Feng","year":"1994","journal-title":"Infect Immun"},{"issue":"4","key":"ppat.1014626.ref059","article-title":"Cutaneous immunoprofiles of three spotted fever group rickettsia cases","volume":"88","author":"N Jia","year":"2020","journal-title":"Infect Immun"},{"issue":"5","key":"ppat.1014626.ref060","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1093\/infdis\/174.5.1051","article-title":"Depression of CD4 T cell subsets and alteration in cytokine profile in boutonneuse fever","volume":"174","author":"E Cillari","year":"1996","journal-title":"J Infect Dis"},{"key":"ppat.1014626.ref061","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.110283","article-title":"Correction: Interferon receptor-deficient mice are susceptible to eschar-associated rickettsiosis","volume":"14","author":"TP Burke","year":"2025","journal-title":"Elife"},{"issue":"9","key":"ppat.1014626.ref062","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.1128\/IAI.00349-09","article-title":"CD4+ CD25+ 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