{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:45:48Z","timestamp":1791917148914,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.ppat.1014669","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000}}],"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570032"],"award-info":[{"award-number":["32570032"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32560046"],"award-info":[{"award-number":["32560046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Double First-class Discipline Promotion Project","award":["2021B10564001"],"award-info":[{"award-number":["2021B10564001"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["20252BAC200397"],"award-info":[{"award-number":["20252BAC200397"]}],"id":[{"id":"https:\/\/ror.org\/04aqat463","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    In\n                    <jats:italic>Xanthomonas<\/jats:italic>\n                    , RpfF is a bifunctional enzyme having both enoyl-ACP dehydratase and acyl-ACP thioesterase activities catalyzed by a single active site. While the dehydratase activity is essential for producing quorum-sensing signals of the diffusible signal factor (DSF) family, the physiological role of the thioesterase activity remained unclear. Singh and coworkers have reported that deletion of the gene encoding RpfF (a \u2206\n                    <jats:italic>rpfF<\/jats:italic>\n                    strain) of the rice pathogen\n                    <jats:italic>X<\/jats:italic>\n                    .\n                    <jats:italic>oryzae<\/jats:italic>\n                    pv.\n                    <jats:italic>oryzae<\/jats:italic>\n                    (\n                    <jats:italic>Xoo<\/jats:italic>\n                    ) resulted in loss of membrane integrity (Singh\n                    <jats:italic>et al<\/jats:italic>\n                    , 2022). We report that the loss in membrane integrity is due to lack of the RpfF thioesterase activity. This was demonstrated by restoration of membrane integrity in the \u0394\n                    <jats:italic>rpfF<\/jats:italic>\n                    strain by expression of a foreign thioesterase. Thioesterases originating in either\n                    <jats:italic>E. coli<\/jats:italic>\n                    or a plant enzyme fully restored virulence in rice, bacterial growth, and tolerance of membrane stress. The thioesterase overcame the defects in membrane lipid synthesis reported previously and restored the lipid components to wild-type levels. Radiolabeling revealed that fatty acid synthesis in the \u0394\n                    <jats:italic>rpfF<\/jats:italic>\n                    strain proceeded at only one-third of the wild-type rate, which was rescued by complementation with thioesterase encoding genes. Thus, the acyl-ACP thioesterase activity of RpfF is a key regulator of\n                    <jats:italic>de novo<\/jats:italic>\n                    fatty acid synthesis and membrane integrity. We propose a dual-function model wherein RpfF maintains membrane lipid homeostasis via its thioesterase activity while concurrently producing DSF signals via its dehydratase activity to orchestrate quorum sensing.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014669","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:47:39Z","timestamp":1791308859000},"page":"e1014669","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The RpfF Acyl-ACP thioesterase of major rice pathogen Xanthomonas oryzae pv. oryzae is essential for maintenance of membrane function and virulence"],"prefix":"10.1371","volume":"22","author":[{"given":"Lizhen","family":"Luo","sequence":"first","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, Guangdong, China"],"department":["Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences"]},{"name":"University of Illinois at Urbana-Champaign","place":["Urbana, Illinois, United States of America"],"department":["Department of Microbiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Hu","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, Guangdong, China"],"department":["Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfeng","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Jiangxi Academy of Agricultural Sciences","place":["Nanchang, Jiangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Cronan","sequence":"additional","affiliation":[{"name":"University of Illinois at Urbana-Champaign","place":["Urbana, Illinois, United States of America"],"department":["Department of Microbiology"]},{"name":"University of Illinois at Urbana-Champaign","place":["Urbana, Illinois, United States of America"],"department":["Department of Biochemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9458-4697","authenticated-orcid":true,"given":"Haihong","family":"Wang","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, Guangdong, China"],"department":["Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life 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fastidiosa","volume":"25","author":"RPP Almeida","year":"2012","journal-title":"Mol Plant Microbe Interact"},{"key":"ppat.1014669.ref030","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev-micro-090816-093754","article-title":"Outer membrane biogenesis","volume":"71","author":"A Konovalova","year":"2017","journal-title":"Annu Rev Microbiol"},{"issue":"7","key":"ppat.1014669.ref031","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nrmicro3047","article-title":"Fortifying the barrier: The impact of lipid A remodelling on bacterial pathogenesis","volume":"11","author":"BD Needham","year":"2013","journal-title":"Nat Rev Microbiol"},{"issue":"13","key":"ppat.1014669.ref032","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00849-16","article-title":"Impact of membrane phospholipid alterations in Escherichia coli on cellular function and bacterial stress adaptation","volume":"199","author":"VW Rowlett","year":"2017","journal-title":"J 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Relation to fabA and suppression of mutations in lipid A biosynthesis","volume":"269","author":"S Mohan","year":"1994","journal-title":"J Biol Chem"},{"issue":"9","key":"ppat.1014669.ref040","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.str.2010.06.011","article-title":"Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis","volume":"18","author":"Z Cheng","year":"2010","journal-title":"Structure"},{"issue":"44","key":"ppat.1014669.ref041","doi-asserted-by":"crossref","first-page":"33414","DOI":"10.1074\/jbc.M606571200","article-title":"Dual signaling functions of the hybrid sensor kinase RpfC of Xanthomonas campestris involve either phosphorelay or receiver domain-protein interaction","volume":"281","author":"Y-W He","year":"2006","journal-title":"J Biol 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Zou","year":"2012","journal-title":"J Bacteriol"},{"issue":"16","key":"ppat.1014669.ref045","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2321498121","article-title":"Engineering a nanoantibiotic system displaying dual mechanism of action","volume":"121","author":"H Xing","year":"2024","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"ppat.1014669.ref046","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1128\/JB.186.6.1869-1878.2004","article-title":"Isolation and characterization of beta-ketoacyl-acyl carrier protein reductase (fabG) mutants of Escherichia coli and Salmonella enterica serovar Typhimurium","volume":"186","author":"C-Y Lai","year":"2004","journal-title":"J Bacteriol"},{"issue":"2","key":"ppat.1014669.ref047","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1128\/AAC.01152-09","article-title":"Triclosan resistance of Pseudomonas aeruginosa PAO1 is due to FabV, a triclosan-resistant enoyl-acyl carrier protein 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