{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:55:52Z","timestamp":1791568552494,"version":"4.3.4"},"reference-count":79,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&D Program of China","award":["2024YFC2309600"],"award-info":[{"award-number":["2024YFC2309600"]}]},{"name":"Major International (Regional) Joint Research Project","award":["W2411075"],"award-info":[{"award-number":["W2411075"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370078"],"award-info":[{"award-number":["32370078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20231281"],"award-info":[{"award-number":["BK20231281"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"name":"General Program of China Postdoctoral Science Foundation","award":["2024M751465"],"award-info":[{"award-number":["2024M751465"]}],"id":[{"id":"https:\/\/ror.org\/0426zh255","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Jiangsu Funding Program for Excellent Postdoctoral Talent","award":["2023ZB683"],"award-info":[{"award-number":["2023ZB683"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Invasive fungal infections pose a serious global health threat. Although core cellular processes are broadly conserved across fungi, only a small fraction of known species cause severe human disease, suggesting that pathogenicity may depend in part on pathogen-specific regulators. However, such elements remain poorly understood because they often lack recognizable homologs, limiting accurate annotation and functional characterization. Here, using the invasive mold\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    as a model, we combined transcriptome-guided re-annotation with functional screening to identify previously unrecognized long non-coding RNAs (lncRNAs) required for fungal growth and virulence. Among 423 loci previously annotated as\n                    <jats:italic>Fumigati<\/jats:italic>\n                    section-specific protein-coding genes, 176 were reclassified as non-coding, revealing widespread annotation errors that had obscured potential regulators. We identified two lncRNAs,\n                    <jats:italic>SSP7<\/jats:italic>\n                    and\n                    <jats:italic>SSP8<\/jats:italic>\n                    , that are required for hyphal growth and virulence in a murine infection model. Mechanistically,\n                    <jats:italic>SSP7<\/jats:italic>\n                    binds the coding region of\n                    <jats:italic>pprA<\/jats:italic>\n                    mRNA, promotes its association with polysomes and translation, and thereby sustains mitochondrial function, whereas\n                    <jats:italic>SSP8<\/jats:italic>\n                    contributes to amino acid homeostasis. Together, these findings demonstrate for the first time that pathogen-specific lncRNAs can fine-tune conserved cellular processes to generate distinct fungal growth and virulence traits, and reveal a novel translational regulatory mechanism in fungal pathogenicity.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014681","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:30:41Z","timestamp":1791567041000},"page":"e1014681","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Large-scale genome re-annotation uncovers pathogen-specific lncRNA regulators of fungal growth and virulence"],"prefix":"10.1371","volume":"22","author":[{"given":"Renwei","family":"Gao","sequence":"first","affiliation":[{"name":"Nanjing Normal University","place":["Nanjing, China"],"department":["Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxiu","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing Normal University","place":["Nanjing, China"],"department":["Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Tang","sequence":"additional","affiliation":[{"name":"Nanjing Normal University","place":["Nanjing, China"],"department":["Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Nanjing Normal University","place":["Nanjing, China"],"department":["Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinying","family":"Xue","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatric Diseases","place":["Beijing, China"],"department":["Department of Respiratory and Critical Care, Xuanwu Hospital of Capital Medical University"]},{"name":"Shandong Second Medical University","place":["Weifang, China"],"department":["Department of Respiratory and Critical Care"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Lu","sequence":"additional","affiliation":[{"name":"Nanjing Normal University","place":["Nanjing, China"],"department":["Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0854-6123","authenticated-orcid":true,"given":"Yuanwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing Normal University","place":["Nanjing, China"],"department":["Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"issue":"5","key":"ppat.1014681.ref001","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/s41564-022-01112-0","article-title":"Evolution of the human pathogenic lifestyle in fungi","volume":"7","author":"A Rokas","year":"2022","journal-title":"Nat 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