{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T17:51:34Z","timestamp":1791827494629,"version":"4.3.4"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32473055"],"award-info":[{"award-number":["32473055"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2023A1515011964 and 2024A1515011658"],"award-info":[{"award-number":["2023A1515011964 and 2024A1515011658"]}]},{"name":"Specific University Discipline Construction Project","award":["2023B10564003"],"award-info":[{"award-number":["2023B10564003"]}]},{"name":"111 Center","award":["D20008"],"award-info":[{"award-number":["D20008"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Nuclear receptor NR1F regulates circadian rhythms in mammals, metamorphosis in insects, and molting in free-living\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    ; however, its role in parasitic nematode development remains elusive.\n                    <jats:italic>Angiostrongylus cantonensis,<\/jats:italic>\n                    a zoonotic pathogen that causes eosinophilic meningitis in humans, exhibits distinct host adaptation, with mice and most mammals serving as non-permissive hosts and rats as the permissive host. We combined multi-omic analyses, lentivirus-mediated RNAi, LC-MS\/MS, and dual-luciferase assays to elucidate\n                    <jats:italic>Ac<\/jats:italic>\n                    NR1F functions in development and host adaptation.\n                    <jats:italic>Acnr1f<\/jats:italic>\n                    was expressed in the intestine, uterus, and seminal vesicle by\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization.\n                    <jats:italic>Acnr1f<\/jats:italic>\n                    showed high expression in L5 larvae and adults but low expression in L1-L4 larvae.\n                    <jats:italic>Acnr1f<\/jats:italic>\n                    expression was significantly higher in worms derived from rats than from mice; approximately 25% of co-expressed genes (918) were identified as potential downstream targets that may be integrated to regulate parasitic adaptation. RNAi reduced\n                    <jats:italic>Acnr1f<\/jats:italic>\n                    transcripts by 38.11% in female adults, resulting in significantly decreased recovery of L4 and L5 larvae and adults, a 64% reduction in egg production, and abnormal eggs with low hatching rate. Histological and ultrastructural analyses revealed thinned cuticle, reduced collagen fibers, and disrupted epidermal integrity. Transcriptomic analysis showed that differentially expressed genes were enriched in structural constituent of cuticle, metalloendopeptidase activity, extracellular matrix structural constituent. Dual-luciferase assays showed that the down-regulated collagen genes\n                    <jats:italic>col-12<\/jats:italic>\n                    and\n                    <jats:italic>col-144<\/jats:italic>\n                    were direct\n                    <jats:italic>Ac<\/jats:italic>\n                    NR1F targets (&gt;300-fold and &gt;40-fold induction, respectively). Differential levels of cholesterol, corticosterone, and 7\u03b1,25-dihydroxycholesterol between worms from rats and mice were identified as\n                    <jats:italic>Ac<\/jats:italic>\n                    NR1F ligands by LC-MS\/MS and ligand-binding assays. Thus,\n                    <jats:italic>Ac<\/jats:italic>\n                    NR1F is a ligand-activated nuclear receptor that directly regulates target genes to influence development and reproduction of\n                    <jats:italic>A. cantonensis<\/jats:italic>\n                    . The distinct availability of host-derived ligands and differential\n                    <jats:italic>Ac<\/jats:italic>\n                    NR1F expressive level between worms from rodents highlights this receptor as a critical molecular switch for parasitic adaptation, providing mechanistic insights into host-pathogen interactions.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1014691","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T17:29:51Z","timestamp":1791826191000},"page":"e1014691","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["AcNR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis"],"prefix":"10.1371","volume":"22","author":[{"given":"Jinwei","family":"Lv","sequence":"first","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Ma","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Lai","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Li","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Ning","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangping","family":"Lu","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Xiao","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyu","family":"Feng","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5992-3016","authenticated-orcid":true,"given":"Dongjuan","family":"Yuan","sequence":"additional","affiliation":[{"name":"South China Agricultural University","place":["Guangzhou, China"],"department":["State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"issue":"8","key":"ppat.1014691.ref001","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1007\/s00436-017-5526-7","article-title":"Angiostrongylus cantonensis: An optimized cultivation of this parasitic nematode under laboratory conditions","volume":"116","author":"H Xie","year":"2017","journal-title":"Parasitol Res"},{"issue":"12","key":"ppat.1014691.ref002","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.3201\/eid1012.040319","article-title":"Parastrongylus cantonensis in a nonhuman primate, Florida","volume":"10","author":"MS Duffy","year":"2004","journal-title":"Emerg Infect 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