{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T06:09:54Z","timestamp":1775714994185,"version":"3.50.1"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2009,3,31]],"date-time":"2009-03-31T00:00:00Z","timestamp":1238457600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosbiology.org"],"crossmark-restriction":false},"short-container-title":["PLoS Biol"],"DOI":"10.1371\/journal.pbio.1000071","type":"journal-article","created":{"date-parts":[[2009,3,30]],"date-time":"2009-03-30T14:18:17Z","timestamp":1238422697000},"page":"e1000071","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":56,"title":["The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions"],"prefix":"10.1371","volume":"7","author":[{"given":"Cedric","family":"Montanier","sequence":"first","affiliation":[]},{"given":"Victoria A","family":"Money","sequence":"additional","affiliation":[]},{"given":"Virginia M. R","family":"Pires","sequence":"additional","affiliation":[]},{"given":"James E","family":"Flint","sequence":"additional","affiliation":[]},{"given":"Benedita A","family":"Pinheiro","sequence":"additional","affiliation":[]},{"given":"Arun","family":"Goyal","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 A. M","family":"Prates","sequence":"additional","affiliation":[]},{"given":"Atsushi","family":"Izumi","sequence":"additional","affiliation":[]},{"given":"Henrik","family":"St\u00e5lbrand","sequence":"additional","affiliation":[]},{"given":"Carl","family":"Morland","sequence":"additional","affiliation":[]},{"given":"Alan","family":"Cartmell","sequence":"additional","affiliation":[]},{"given":"Katarina","family":"Kolenova","sequence":"additional","affiliation":[]},{"given":"Evangelos","family":"Topakas","sequence":"additional","affiliation":[]},{"given":"Eleanor J","family":"Dodson","sequence":"additional","affiliation":[]},{"given":"David N","family":"Bolam","sequence":"additional","affiliation":[]},{"given":"Gideon J","family":"Davies","sequence":"additional","affiliation":[]},{"given":"Carlos M. G. A","family":"Fontes","sequence":"additional","affiliation":[]},{"given":"Harry J","family":"Gilbert","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2009,3,31]]},"reference":[{"key":"journal-pbio-1000071-b001","first-page":"484","volume":"311","author":"Ragauskas","year":"2006","journal-title":"Science","ISSN":"https:\/\/id.crossref.org\/issn\/0193-4511","issn-type":"print"},{"key":"journal-pbio-1000071-b002","first-page":"315","volume":"17","author":"Sticklen","year":"2006","journal-title":"Curr Opin Biotechnol","ISSN":"https:\/\/id.crossref.org\/issn\/0958-1669","issn-type":"print"},{"key":"journal-pbio-1000071-b003","author":"Brett","year":"1996","journal-title":"Topics in Plant Functional Biology;"},{"key":"journal-pbio-1000071-b004","first-page":"1","volume":"37","author":"Tomme","year":"1995","journal-title":"Adv Microb Physiol","ISSN":"https:\/\/id.crossref.org\/issn\/0065-2911","issn-type":"print"},{"key":"journal-pbio-1000071-b005","first-page":"183","volume":"50","author":"Warren","year":"1996","journal-title":"Annu Rev Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4227","issn-type":"print"},{"key":"journal-pbio-1000071-b006","first-page":"749","volume":"309","author":"Hall","year":"1995","journal-title":"Biochem J","ISSN":"https:\/\/id.crossref.org\/issn\/0264-6021","issn-type":"print"},{"key":"journal-pbio-1000071-b007","first-page":"3","author":"Coutinho","year":"1999","journal-title":"Recent Advances in Carbohydrate Bioengineering”."},{"key":"journal-pbio-1000071-b008","first-page":"309","volume":"280","author":"Henrissat","year":"1991","journal-title":"Biochem J","ISSN":"https:\/\/id.crossref.org\/issn\/0264-6021","issn-type":"print"},{"key":"journal-pbio-1000071-b009","first-page":"2605","volume":"143","author":"Dalrymple","year":"1997","journal-title":"Microbiology","ISSN":"https:\/\/id.crossref.org\/issn\/0026-2617","issn-type":"print"},{"key":"journal-pbio-1000071-b010","first-page":"521","volume":"58","author":"Bayer","year":"2004","journal-title":"Annu Rev Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4227","issn-type":"print"},{"key":"journal-pbio-1000071-b011","first-page":"29","volume":"69","author":"Hall","year":"1988","journal-title":"Gene","ISSN":"https:\/\/id.crossref.org\/issn\/0378-1119","issn-type":"print"},{"key":"journal-pbio-1000071-b012","first-page":"289","volume":"273","author":"Durrant","year":"1991","journal-title":"Biochem J","ISSN":"https:\/\/id.crossref.org\/issn\/0264-6021","issn-type":"print"},{"key":"journal-pbio-1000071-b013","first-page":"2074","volume":"299","author":"Xu","year":"2003","journal-title":"Science","ISSN":"https:\/\/id.crossref.org\/issn\/0193-4511","issn-type":"print"},{"key":"journal-pbio-1000071-b014","author":"DeBoy","year":"2008","journal-title":"Insights into Plant Cell-wall Degradation from the Genome Sequence of the Soil Bacterium Cellvibrio japonicus"},{"key":"journal-pbio-1000071-b015","first-page":"534","volume":"43","author":"Akoh","year":"2004","journal-title":"Prog Lipid Res","ISSN":"https:\/\/id.crossref.org\/issn\/0163-7827","issn-type":"print"},{"key":"journal-pbio-1000071-b016","first-page":"2256","volume":"D60","author":"Krissinel","year":"2004","journal-title":"Acta Crystallogr","ISSN":"https:\/\/id.crossref.org\/issn\/0365-110X","issn-type":"print"},{"key":"journal-pbio-1000071-b017","first-page":"10184","volume":"45","author":"Larsen","year":"2006","journal-title":"Biochemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0006-2960","issn-type":"print"},{"key":"journal-pbio-1000071-b018","first-page":"345","author":"Wharton","year":"1997","journal-title":"Comprehensive Biological Catalysis"},{"key":"journal-pbio-1000071-b019","first-page":"769","volume":"382","author":"Boraston","year":"2004","journal-title":"Biochem J","ISSN":"https:\/\/id.crossref.org\/issn\/0264-6021","issn-type":"print"},{"key":"journal-pbio-1000071-b020","first-page":"312","volume":"429","author":"Nagy","year":"1998","journal-title":"FEBS Lett","ISSN":"https:\/\/id.crossref.org\/issn\/0014-5793","issn-type":"print"},{"key":"journal-pbio-1000071-b021","first-page":"985","volume":"39","author":"Ponyi","year":"2000","journal-title":"Biochemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0006-2960","issn-type":"print"},{"key":"journal-pbio-1000071-b022","first-page":"23718","volume":"280","author":"Flint","year":"2005","journal-title":"J Biol Chem","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"key":"journal-pbio-1000071-b023","first-page":"R91","volume":"6","author":"O'Brien","year":"1999","journal-title":"Chem Biol","ISSN":"https:\/\/id.crossref.org\/issn\/1074-5521","issn-type":"print"},{"key":"journal-pbio-1000071-b024","first-page":"73","volume":"37","author":"Aharoni","year":"2005","journal-title":"Nat Genet","ISSN":"https:\/\/id.crossref.org\/issn\/1061-4036","issn-type":"print"},{"key":"journal-pbio-1000071-b025","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-86659-3","author":"Ohno","year":"1970","journal-title":"Evolution by gene duplication"},{"key":"journal-pbio-1000071-b026","first-page":"2848","volume":"71","author":"Kimura","year":"1974","journal-title":"Proc Natl Acad Sci U S A","ISSN":"https:\/\/id.crossref.org\/issn\/1091-6490","issn-type":"print"},{"key":"journal-pbio-1000071-b027","first-page":"3479","volume":"85","author":"Piatigorsky","year":"1988","journal-title":"Proc Natl Acad Sci U S A","ISSN":"https:\/\/id.crossref.org\/issn\/1091-6490","issn-type":"print"},{"key":"journal-pbio-1000071-b028","first-page":"17004","volume":"104","author":"Bergthorsson","year":"2007","journal-title":"Proc Natl Acad Sci U S A","ISSN":"https:\/\/id.crossref.org\/issn\/1091-6490","issn-type":"print"},{"key":"journal-pbio-1000071-b029","first-page":"2311","volume":"25","author":"Bershtein","year":"2008","journal-title":"Mol Biol Evol","ISSN":"https:\/\/id.crossref.org\/issn\/0737-4038","issn-type":"print"},{"key":"journal-pbio-1000071-b030","first-page":"85","volume":"320","author":"Wang","year":"2002","journal-title":"J Mol Biol","ISSN":"https:\/\/id.crossref.org\/issn\/0022-2836","issn-type":"print"},{"key":"journal-pbio-1000071-b031","first-page":"973","volume":"11","author":"Robert","year":"2003","journal-title":"Structure","ISSN":"https:\/\/id.crossref.org\/issn\/0969-2126","issn-type":"print"},{"key":"journal-pbio-1000071-b032","first-page":"623","volume":"12","author":"Allouch","year":"2004","journal-title":"Structure","ISSN":"https:\/\/id.crossref.org\/issn\/0969-2126","issn-type":"print"},{"key":"journal-pbio-1000071-b033","first-page":"5013","volume":"39","author":"Charnock","year":"2000","journal-title":"Biochemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0006-2960","issn-type":"print"},{"key":"journal-pbio-1000071-b034","first-page":"551","volume":"160","author":"Johnson","year":"1988","journal-title":"Methods Enzymol","ISSN":"https:\/\/id.crossref.org\/issn\/0076-6879","issn-type":"print"},{"key":"journal-pbio-1000071-b035","first-page":"29","volume":"48","author":"Lundqvist","year":"2002","journal-title":"Carbohydr Poly","ISSN":"https:\/\/id.crossref.org\/issn\/0144-8617","issn-type":"print"},{"key":"journal-pbio-1000071-b036","first-page":"22953","volume":"279","author":"Bolam","year":"2004","journal-title":"J Biol Chem","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"key":"journal-pbio-1000071-b037","first-page":"307","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol","ISSN":"https:\/\/id.crossref.org\/issn\/0076-6879","issn-type":"print"},{"key":"journal-pbio-1000071-b039","first-page":"1772","volume":"58","author":"Schneider","year":"2002","journal-title":"Acta Crystallographica Section D-Biological Crystallography","ISSN":"https:\/\/id.crossref.org\/issn\/0907-4449","issn-type":"print"},{"key":"journal-pbio-1000071-b040","first-page":"458","volume":"6","author":"Perrakis","year":"1999","journal-title":"Nature Structural Biology","ISSN":"https:\/\/id.crossref.org\/issn\/1072-8368","issn-type":"print"},{"key":"journal-pbio-1000071-b041","first-page":"240","volume":"53","author":"Murshudov","year":"1997","journal-title":"Acta Cryst D","ISSN":"https:\/\/id.crossref.org\/issn\/0907-4449","issn-type":"print"},{"key":"journal-pbio-1000071-b042","first-page":"2126","volume":"D60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr","ISSN":"https:\/\/id.crossref.org\/issn\/0365-110X","issn-type":"print"},{"key":"journal-pbio-1000071-b043","first-page":"658","volume":"40","author":"McCoy","year":"2007","journal-title":"Journal of Applied Crystallography","ISSN":"https:\/\/id.crossref.org\/issn\/0021-8898","issn-type":"print"}],"container-title":["PLoS Biology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T20:49:53Z","timestamp":1540327793000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.1000071"}},"subtitle":[],"editor":[{"given":"Daniel","family":"Herschlag","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2009,3,31]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,3,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pbio.1000071","relation":{},"ISSN":["1545-7885"],"issn-type":[{"value":"1545-7885","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3,31]]}}}